Pathak, Rachana Bhangu, Sukhvir Kaur Martin, Gregory J O Separovic, Frances Ashokkumar, Muthupandian
Published in
European biophysics journal : EBJ
Amyloid crystals, a form of ordered protein aggregates documented relatively recently, have not been studied as extensively as amyloid fibres. This study investigates the formation of amyloid crystals with low frequency ultrasound (20 kHz) using β-lactoglobulin, as a model protein for amyloid synthesis. Acoustic cavitation generates localised zones...
Lee, Anthony G.
Published in
European Biophysics Journal
Scap and Insig, two proteins embedded in the membrane of the endoplasmic reticulum (ER), regulate the synthesis of cholesterol in animal cells by forming a dimer in the presence of high concentrations of cholesterol. Cryo-electron microscopic structures for the Scap–Insig dimer show a sterol-binding site at the dimer interface, but none of the stru...
Piselli, Claudio Benier, Lorraine Koy, Cornelia Glocker, Michael O. Benz, Roland
Published in
European Biophysics Journal
The cell wall of Rhodococcus corynebacteroides formerly known as Nocardia corynebacteroides contains cell wall channels that are responsible for the cell wall permeability of this bacterium. Based on partial sequencing of the polypeptide subunits and a BLAST search, we identified one polypeptide of R. corynebacteroides (PorARc) and two polypeptides...
Yao, Shenggen Keizer, David W. Babon, Jeffrey J. Separovic, Frances
Published in
European Biophysics Journal
Defining protein oligomeric state and/or its changes in solution is of significant interest for many biophysical studies carried out in vitro, especially when the nature of the oligomeric state is crucial in the subsequent interpretation of experimental results and their biological relevance. Nuclear magnetic resonance (NMR) is a well-established m...
Pechlaner, Maria van Gunsteren, Wilfred F Hansen, Niels Smith, Lorna J
Published in
European biophysics journal : EBJ
In protein simulation or structure refinement based on values of observable quantities measured in (aqueous) solution, solvent (water) molecules may be explicitly treated, omitted, or represented by a potential of mean-solvation-force term, depending on protein coordinates only, in the force field used. These three approaches are compared for hen e...
Wang, Haoqing Obeidy, Peyman Wang, Zihao Zhao, Yunduo Wang, Yao Su, Qian Peter Cox, Charles D. Ju, Lining Arnold
Published in
European Biophysics Journal
Mechanical stimuli such as tension, compression, and shear stress play critical roles in the physiological functions of red blood cells (RBCs) and their homeostasis, ATP release, and rheological properties. Intracellular calcium (Ca2+) mobilization reflects RBC mechanosensing as they transverse the complex vasculature. Emerging studies have demonst...
Tortorella, Ilaria Argentati, Chiara Emiliani, Carla Martino, Sabata Morena, Francesco
Published in
European biophysics journal : EBJ
Organoids are a novel three-dimensional stem cells' culture system that allows the in vitro recapitulation of organs/tissues structure complexity. Pluripotent and adult stem cells are included in a peculiar microenvironment consisting of a supporting structure (an extracellular matrix (ECM)-like component) and a cocktail of soluble bioactive molecu...
Wang, Haoqing Zhou, Fang Guo, Yuze Ju, Lining Arnold
Published in
European Biophysics Journal
Mechanobiology is an emerging field at the interface of biology and mechanics, investigating the roles of mechanical forces within biomolecules, organelles, cells, and tissues. As a highlight, the recent advances of micropipette-based aspiration assays and dynamic force spectroscopies such as biomembrane force probe (BFP) provide unprecedented mech...
Burn, Andreas Schneiter, Martin Ryser, Manuel Gehr, Peter Rička, Jaroslav Frenz, Martin
Published in
European Biophysics Journal
Collectively coordinated ciliary activity propels the airway mucus, which lines the luminal surface of the vertebrate respiratory system, in cranial direction. Our contemporary understanding on how the quantitative characteristics of the metachronal wave field determines the resulting mucociliary transport is still limited, partly due to the sparse...
Benz, Roland Piselli, Claudio Hoxha, Cezarela Koy, Cornelia Glocker, Michael O Popoff, Michel R
Published in
European biophysics journal : EBJ
Clostridium perfringens is a potent producer of a variety of toxins. Well studied from these are five toxins (alpha, Beta (CPB), epsilon, iota and CPE) that are produced by seven toxinotype strains (A-G) of C. perfringens. Besides these toxins, C. perfringens produces also another toxin that causes necrotizing enterocolitis in piglets. This toxin t...
