Carlier, F. Li, M. Maroc, L. Debuchy, R. Souaid, C. Noordermeer, D. Grognet, P. Malagnac, F.
Published in
Epigenetics & Chromatin
BackgroundSelective gene silencing is key to development. It is generally accepted that H3K27me3-enriched heterochromatin maintains transcriptional repression established during early development and regulates cell fate. Conversely, H3K9me3-enriched heterochromatin prevents differentiation but constitutes protection against transposable elements. W...
Golov, Arkadiy K. Golova, Anastasia V. Gavrilov, Alexey A. Razin, Sergey V.
Published in
Epigenetics & Chromatin
Cohesin is a key organizer of chromatin folding in eukaryotic cells. The two main activities of this ring-shaped protein complex are the maintenance of sister chromatid cohesion and the establishment of long-range DNA–DNA interactions through the process of loop extrusion. Although the basic principles of both cohesion and loop extrusion have been ...
Decombe, Sheldon Loll, François Caccianini, Laura Affannoukoué, Kévin Izeddin, Ignacio Mozziconacci, Julien Escudé, Christophe Lopes, Judith
Published in
Epigenetics & Chromatin
BackgroundCentromeric regions of human chromosomes contain large numbers of tandemly repeated α-satellite sequences. These sequences are covered with constitutive heterochromatin which is enriched in trimethylation of histone H3 on lysine 9 (H3K9me3). Although well studied using artificial chromosomes and global perturbations, the contribution of t...
Lian, Yinjie Hao, Huijuan Xu, Jing Bo, Tao Liang, Aihua Wang, Wei
Published in
Epigenetics & Chromatin
Histone chaperones facilitate DNA replication and repair by promoting chromatin assembly, disassembly and histone exchange. Following histones synthesis and nucleosome assembly, the histones undergo posttranslational modification by different enzymes and are deposited onto chromatins by various histone chaperones. In Tetrahymena thermophila, histon...
Kananen, Laura Marttila, Saara
Published in
Epigenetics & Chromatin
BackgroundAgeing displays clear sexual dimorphism, evident in both morbidity and mortality. Ageing is also associated with changes in DNA methylation, but very little focus has been on the sex chromosomes, potential biological contributors to the observed sexual dimorphism. Here, we sought to identify DNA methylation changes associated with ageing ...
Uğurlu-Çimen, Deniz Odluyurt, Deniz Sevinç, Kenan Özkan-Küçük, Nazlı Ezgi Özçimen, Burcu Demirtaş, Deniz Enüstün, Eray Aztekin, Can Philpott, Martin Oppermann, Udo
...
Published in
Epigenetics & Chromatin
BackgroundThe histone H3 lysine 79 (H3K79) methyltransferase DOT1L is a key chromatin-based barrier to somatic cell reprogramming. However, the mechanisms by which DOT1L safeguards cell identity and somatic-specific transcriptional programs remain unknown.ResultsWe employed a proteomic approach using proximity-based labeling to identify DOT1L-inter...
Fitzgerald, Eamon Sinton, Matthew C. Wernig-Zorc, Sara Morton, Nicholas M. Holmes, Megan C. Boardman, James P. Drake, Amanda J.
Published in
Epigenetics & Chromatin
Exposure to early life stress (ELS) during childhood or prenatally increases the risk of future psychiatric disorders. The effect of stress exposure during the neonatal period is less well understood. In preterm infants, exposure to invasive procedures is associated with altered brain development and future stress responses suggesting that the neon...
Balaton, Bradley P. Brown, Carolyn J.
Published in
Epigenetics & Chromatin
BackgroundX-chromosome inactivation (XCI) is the epigenetic inactivation of one of two X chromosomes in XX eutherian mammals. The inactive X chromosome is the result of multiple silencing pathways that act in concert to deposit chromatin changes, including DNA methylation and histone modifications. Yet over 15% of genes escape or variably escape fr...
Jordan, William III Larschan, Erica
Published in
Epigenetics & Chromatin
BackgroundDrosophila dosage compensation is an important model system for defining how active chromatin domains are formed. The male-specific lethal dosage compensation complex (MSLc) increases transcript levels of genes along the length of the single male X-chromosome to equalize with that expressed from the two female X-chromosomes. The strongest...
Morrison, Jacob Koeman, Julie M. Johnson, Benjamin K. Foy, Kelly K. Beddows, Ian Zhou, Wanding Chesla, David W. Rossell, Larissa L. Siegwald, Emily J. Adams, Marie
...
Published in
Epigenetics & Chromatin
BackgroundWith rapidly dropping sequencing cost, the popularity of whole-genome DNA methylation sequencing has been on the rise. Multiple library preparation protocols currently exist. We have performed 22 whole-genome DNA methylation sequencing experiments on snap frozen human samples, and extensively benchmarked common library preparation protoco...
