Ordeñana Manso, Julen Nielsen, Martin B Balaguer Moya, Eva Sandri, Juliana P Yamakawa, Celina K Mussatto, Solange I
Published in
Enzyme and microbial technology
The transition from an economic model based on resource extraction to a more sustainable and circular economy requires the development of innovative methods to unlock the potential of raw materials such as lignocellulosic biomasses. Corn fiber differs from more traditional lignocellulosic biomasses due to its high starch content, which provides add...
Xu, Xinqi Chen, Tianheng Xu, Lian Lin, Juan
Published in
Enzyme and microbial technology
Laccase is an efficient biocatalyst for oxidative polymerization of organic substrates. However, cost of enzyme preparation, low stability and residual protein diminish the efficiency of laccase mediated polymerization. In this work, a series of silicon dioxide coated ferroferric oxide magnetic nanoparticles were modified by different functional gr...
Zhang, Lingzhi Zhou, Rui Fu, Xiaoli Zhang, Guangya Zhang, Lijuan Zhou, Shu-Feng Jiang, Wei
Published in
Enzyme and microbial technology
The synthesis of chiral intermediates for the traditional antidepressant duloxetine has gained significant attention as the number of depressed patients continues to grow. S-N, N-Dimethyl-3-hydroxy-3-(2-thienyl)-1-propanamide (S-DHTP) is a critical intermediate in the synthesis of duloxetine, and the chemical synthesis process is complex and enviro...
Silva, Erica C Rodrigues, Lucas M R Vila, Marta M D C Balcão, Victor M
Published in
Enzyme and microbial technology
Coffee canker, or bacterial halo blight (BHB) of coffee, is a disease caused by the phytopathogenic bacterium Pseudomonas syringae pv. garcae (Psg), having been found for the first time in 1955, in the Garça region (State of São Paulo), and which has stood out in the Brazilian coffee plantations in recent years, leading to severe economic losses th...
Üstüntanır Dede, Ayça Fulya Arslanyolu, Muhittin
Published in
Enzyme and microbial technology
Alternative cell factories, such as the unicellular ciliate eukaryotic Tetrahymena thermophila, may be required for the production of protein therapeutics that are challenging to produce in conventional expression systems. T. thermophila (Tt) can secrete proteins with the post-translational modifications necessary for their function in humans. In t...
Kim, Byungchan Oh, Suk Jin Hwang, Jeong Hyeon Kim, Hyun Jin Shin, Nara Joo, Jeong Chan Choi, Kwon-Young Park, See-Hyoung Park, Kyungmoon Bhatia, Shashi Kant
...
Published in
Enzyme and microbial technology
One of the key intermediates, 5-hydroxyvaleric acid (5-HV), is used in the synthesis of polyhydroxyalkanoate monomer, δ-valerolactone, 1,5-pentanediol (1,5-PDO), and many other substances. Due to global environmental problems, eco-friendly bio-based synthesis of various platform chemicals and key intermediates are socially required, but few previou...
Gupta, Pragya Sahoo, P C Sandipam, Srikanth Gupta, Ravi Prakash Kumar, Manoj
Published in
Enzyme and microbial technology
Biodiesel production from used cooking oil is sustainable alternative, for bio-energy production. The process generates residual crude glycerol (RCG) as the major energy-rich waste which can be used to produce various bio-based chemicals like 1,3-propanediol (1,3-PDO) through biotechnological interventions. This RCG contains several impurities like...
Yan, Yi Bai, Yajun Zheng, Xiaohui Cai, Yujie
Published in
Enzyme and microbial technology
Hydroxytyrosol (HT), a polyphenolic molecule of high value, is used in the nutraceutical, cosmetic, food, and livestock nutrition industries. As a natural product, HT is chemically manufactured or extracted from olives; nevertheless, the increasing demand mandates the exploration and development of alternative sources, such as heterologous producti...
Liu, Shuaifeng Zhang, Meng Hong, Dengwang Fang, Zemin Xiao, Yazhong Fang, Wei Zhang, Xuecheng
Published in
Enzyme and microbial technology
β-Glucosidases with high catalytic activity and glucose tolerant properties possess promising applications in lignocellulose-based industries. To obtain enzymes possessing these properties, a semi-rational strategy was employed to engineer the glucose-stimulating β-glucosidase Bgl2A for high cellobiose hydrolysis activity. A total of 18 mutants wer...
Gan, Tian Fang, Jingyi Wang, Yuxin Liu, Kaiqiang Sang, Yumin Chen, Hanchi Lu, Yuele Zhu, Linjiang Chen, Xiaolong
Published in
Enzyme and microbial technology
2-O-α-D-glucopyranosyl-L-ascorbic acid (AA-2G), a stable glucoside derivative of L-ascorbic acid (L-AA), can be one-step synthesized by sucrose phosphorylase (SPase). In this study, we attempted to produce extracellular SPase in Bacillus subtilis WB800 for the food-grade production of AA-2G. The results showed that the secretion of SPases did not r...
