Published in Electrophoresis
Recombinant human erythropoietin (rhuEPO) has been extensively used as a pharmaceutical product for treating anemia in the clinic. Glycosylation of rhuEPO was crucial for affecting biological activity, immunogenicity and pharmacokinetics. Because of the heterogeneity of glycan, the structure of rhuEPO was complex with several isoforms. Characteriza...
Published in Electrophoresis
Electrospraying (ES) is a potential-driven process of liquid atomization, which is employed in the field of analytical chemistry, particularly as an ionization technique for mass spectrometric analyses of biomolecules. In this review, we demonstrate the extraordinary versatility of the electrospray by overviewing the specifics and advanced applicat...
Published in Electrophoresis
This study aimed to evaluate the polyphenolic composition along with the biological activity of guabiroba (Campomanesia xanthocarpa Berg.) fruits using comprehensive two-dimensional liquid chromatography (LC×LC). A simplex centroid design comprising three solvents (methanol, 2% acetic acid and acetonitrile) was used to optimize the extraction mixtu...
Published in Electrophoresis
The material properties of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its proteins are discussed. We review the viral structure, size, rigidity, lipophilicity, isoelectric point, buoyant density and centrifugation conditions, stability against pH, temperature, UV light, gamma radiation, and susceptibility to various chemic...
Published in Electrophoresis
The human proteome is composed of a diverse and heterogeneous range of gene products/proteoforms/protein species. Because of the growing amount of information about proteoforms generated by different methods we need a convenient approach to make an inventory of the data. Here, we present a database of proteoforms that is based on information obtain...
Published in Electrophoresis
Published in Electrophoresis
Multienzyme detection and monitoring enzyme activity in situ are significant for the disease to diagnose. This study aims to develop a quantum dots (QDs)-based nanoprobe Cyanine5-DDDLEVLFQFPGLVPRGSGGHHHHHH-QDs (Cy5-LEVLVP-QD), which is able to detect two enzymes inside a bent capillary using CE. Cy5-LEVLVP and QDs were allowed to bind with each oth...
Published in Electrophoresis
Raman spectral detection has emerged as a powerful analytical technique due to the advantages of fast acquisition, non-invasion and low cost. The on-site application is highly dependent on Raman automatic analysis algorithm. However, current Raman algorithm research mainly focuses on small sample Raman spectroscopy identification with defects of lo...
Published in Electrophoresis
ABO hemolytic disease of the newborn (ABO-HDN), which may cause neonatal jaundice and polycythemia, or even stillbirth or neonatal death, is widespread in China. Prenatal testing for the fetal ABO blood group can reduce unnecessary concerns or ensure prompt treatment. Herein, we presented a method to employ high-density silica microbeads (SiO2 MBs)...
Published in Electrophoresis
Plasmonic nanomaterials possessing large-volume, high-density hot spots with high field enhancement are highly desirable for ultrasensitive surface-enhanced Raman scattering (SERS) sensing. However, many as-prepared plasmonic nanomaterials are limited in available dense hot spots and in sample size, which greatly hinder their wide applications in S...
