Drouin, Nicolas Mielcarek, Andreas Wenz, Christian Rudaz, Serge
Published in
Electrophoresis
Currently, feature annotation remains one of the main challenges in untargeted metabolomics. In this context, the information provided by high-resolution mass spectrometry (HRMS) in addition to accurate mass can improve the quality of metabolite annotation, and MS/MS fragmentation patterns are widely used. Accurate mass and a separation index, such...
Römer, Jennifer Kiessig, Steffen Moritz, Bernd Neusüß, Christian
Published in
Electrophoresis
Capillary sieving electrophoresis utilizing SDS (CE(SDS)) is one of the most applied methods for the analysis of antibody (mAb) size heterogeneity in the biopharmaceutical industry. Inadequate peak identification of observed protein fragments is still a major issue. In a recent publication, we introduced an electrophoretic 2D system, enabling onlin...
Höcker, Oliver Knierman, Mike Meixner, Jens Neusüß, Christian
Published in
Electrophoresis
CE hyphenated to ESI-MS (CE-ESI-MS) is a well-established technique to analyze charged analytes in complex samples. Although various interfaces for CE-MS coupling are commercially available, the development of alternatives which combine sensitivity, simplicity, and robustness remains a topic of research. In this work, a nanoflow sheath liquid CE-MS...
Kutlucinar, Kaan Georg Hann, Stephan
Published in
Electrophoresis
Nontargeted analysis of water samples using liquid chromatography combined with high‐resolution mass spectrometers is an emerging approach for surface water monitoring and evaluation of water treatment processes. In this study, sample preconcentration via direct, large volume injection with 500 μL and 1000 μL injection volumes was compared to SPE r...
Mlynek, Franz Himmelsbach, Markus Buchberger, Wolfgang Klampfl, Christian W.
Published in
Electrophoresis
The (tentative) identification of unknown drug‐related phase II metabolites in plants upon drug uptake remains a challenging task despite improved analytical instrument performance. To broaden the knowledge of possible drug metabolization, a fast‐screening approach for the tentative identification of drug‐related phase II metabolites is presented i...
Romero-Soto, Fabian O Polanco-Oliva, Maria I Gallo-Villanueva, Roberto C Martinez-Chapa, Sergio O Perez-Gonzalez, Victor H
Published in
Electrophoresis
Cancer is one of the leading causes of annual deaths worldwide, accounting for nearly 10 million deaths each year. Metastasis, the process by which cancer spreads across the patient's body, is the main cause of death in cancer patients. Because the rising trend observed in statistics of new cancer cases and cancer-related deaths does not allow for ...
Amorim, Tatiane Lima Duarte, Lucas Mattos da Silva, Erick Mendes de Oliveira, Marcone Augusto Leal
Published in
Electrophoresis
Fatty acids determination is of paramount importance for quality control and suitable labeling of edible oils, required by regulatory agencies in several countries, and fast methods for this determination are worldly desired. This review article aimed to explore the available analytical methods for vegetable and marine oils analyses employing CE, w...
Galpayage Dona, Kalpani N U Du, E Wei, Jianning
Published in
Electrophoresis
Mitochondrial dynamics (fission and fusion) plays an important role in cell functions. Disruption in mitochondrial dynamics has been associated with diseases such as neurobiological disorders and cardiovascular diseases. Analysis of mitochondrial fission/fusion has been mostly achieved through direct visualization of the fission/fusion events in li...
Ramirez-Murillo, Cinthia J de Los Santos-Ramirez, J Martin Perez-Gonzalez, Victor H
Published in
Electrophoresis
Dielectrophoretically driven microfluidic devices have demonstrated great applicability in biomedical engineering, diagnostic medicine, and biological research. One of the potential fields of application for this technology is in point-of-care (POC) devices, ideally allowing for portable, fully integrated, easy to use, low-cost diagnostic platforms...
Ji, Xiaohui Huang, Sisi Zhang, Jie Bruce, Terri F Tan, Zhijing Wang, Donglin Zhu, Jianhui Marcus, R Kenneth Lubman, David M
Published in
Electrophoresis
We have developed a rapid, low-cost, and simple separation strategy to separate extracellular vesicles (EVs) from a small amount of serum (i.e.,
