van der Burg, Debbie Josefsson, Leila Mikkonen, Saara Chotteau, Véronique Emmer, Åsa Wätzig, Hermann Sänger-van de Griend, Cari E
Published in
Electrophoresis
The rapidly growing, competitive biopharmaceutical market requires tight bioprocess monitoring. An integrated, automated platform for the routine online/at-line monitoring of key factors in the cell culture medium could greatly improve process monitoring. Mono- and disaccharides, as the main energy and carbon source, are one of these key factors. A...
Villemet, Loïc Cuchet, Aurélien Desvignes, Christophe Sänger-van de Griend, Cari E
Published in
Electrophoresis
Protein separation can be achieved with different modes of capillary electrophoresis, such as with capillary gel electroporesis (CGE) or with capillary zone electrophoresis (CZE). CZE protein mapping of peanut extract was approached in four different ways, combining neutral-coated or multilayer-coated capillaries with pHs well over or under the iso...
Pauter, Katarzyna Szultka-Młyńska, Małgorzata Szumski, Michał Król-Górniak, Anna Pomastowski, Paweł Buszewski, Bogusław
Published in
Electrophoresis
In this study, a new analytical method was developed and validated for the simultaneous analysis of antibiotic drugs (amoxicillin, cefotaxime, ciprofloxacin, clindamycin, linezolid, metronidazole) and their metabolites (amoxycilloic acid, amoxicillin diketopiperazine, 3-desacetyl cefotaxime lactone, clindamycin sulfoxide, ciprofloxacin piperazinyl-...
Pieckowski, Michał Kowalski, Piotr Olędzka, Ilona Miękus-Purwin, Natalia Plenis, Alina Roszkowska, Anna Bączek, Tomasz
Published in
Electrophoresis
Mitotane is a cytotoxic drug used in the treatment of inoperable adrenocortical carcinoma, it inhibits steroidogenesis as well, and therefore monitoring the level of steroid hormones in patients treated with mitotane is a crucial point of therapy. Hence, we have developed a simple, fast, and efficient electrophoretic method combined with reverse po...
Kaya, S Irem Cetinkaya, Ahmet Caglayan, Mehmet G Ozkan, Sibel A
Published in
Electrophoresis
Biopharmaceuticals (recombinant technology-based products, vaccines, whole blood and blood components, gene therapy, cells, tissues, etc.,) are described as biological medical products produced from various living sources such as human, microbial, animal, and so on by manufacturing, extraction, or semi-synthesis. They are complex molecules having h...
Piestansky, Juraj Cizmarova, Ivana Matuskova, Michaela Mikus, Peter
Published in
Electrophoresis
The possibility to investigate analytes at ultra-low concentration levels still remains a hot topic in bioanalysis. In this area, various preconcentration techniques are an integral part of analytical procedures. When applying electromigration separation techniques, an isotachophoresis has been advantageously employed many times for this purpose. T...
Mahapatra, Bimalendu Bandopadhyay, Aditya
Published in
Electrophoresis
We report a numerical study on the effect of the skimming layer in an EOF of Oldroyd-B fluid over charge modulated walls. Three types of flow conditions were identified on the basis of the relative thickness of the skimming layer and the electrical double layer. We observe maximum slip velocity magnitude when the skimming layer thickness is very le...
Ohshima, Hiroyuki
Published in
Electrophoresis
The general expression is derived for the diffusiophoretic velocity of a spherical colloidal particle of radius a in a concentration gradient of symmetrical electrolyte. On the basis of this expression, simple approximate analytic expressions for the diffusiophoretic velocity correct up to the order of 1/κa is derived, where κ is the Debye-Hückel p...
Saha, Kalyan Murthy, P V S N Chakraborty, Suman
Published in
Electrophoresis
We investigate electrokinetic transport of power-law fluids in a narrow cylindrical capillary in the presence of a transverse magnetic field. The governing equations including the full Poisson-Boltzmann equation and the Cauchy momentum equation with power-law constitutive behavior are solved numerically, without being restrictive to low surface pot...
Maurya, Saurabh Kumar Sarkar, Sankar Mondal, Hemanta Kumar Ohshima, Hiroyuki Gopmandal, Partha P
Published in
Electrophoresis
Electrophoresis of core-shell composite soft particles possessing hydrophobic inner core grafted with highly charged polyelectrolyte layer (PEL) has been studied analytically. The PEL bears pH-dependent charge properties due to the presence of zwitterionic functional groups. The dielectric permittivity of the PEL and bulk aqueous medium were taken ...
