Bikos, Dimitri A Mason, Thomas G
Published in
Electrophoresis
When determining the electric field E acting on charged objects in gel electrophoresis, the electrical conductivity of the buffer solution is often overlooked; E is typically calculated by dividing the applied voltage by a separation distance between electrodes. However, as a consequence of electrolytic reactions, which occur at the electrodes, gra...
Ren, Yukun Liu, Xianyu Liu, Weiyu Tao, Ye Jia, Yankai Hou, Likai Li, Wenying Jiang, Hongyuan
Published in
Electrophoresis
We report herein a novel microfluidic particle concentrator that utilizes constriction microchannels to enhance the flow-focusing performance of induced-charge electroosmosis (ICEO), where viscous hemi-spherical oil droplets are embedded within the mainchannel to form deformable converging-diverging constriction structures. The constriction region ...
Dutta, Debashis
Published in
Electrophoresis
The use of an electric field in free-flow zone electrophoresis (FFZE) automatically leads to Joule heating yielding a higher temperature at the center of the separation chamber relative to that around the channel walls. For small amounts of heat generated, this thermal effect introduces a variation in the equilibrium position of the analyte molecul...
Gong, Jun-Hua Gong, Jian-Ping Zheng, Kai-Wen
Published in
Electrophoresis
The ability to determine that successful transfer and equal loading occur prior to using primary antibodies is important. And total protein staining is commonly used to check transfer efficiency and normalization, which play a crucial role in western blotting. Ponceau S and coomassie blue are commonly used, but there are disadvantages reported in r...
Zaffino, Rosa Letizia Mir, Mònica Samitier, Josep
Published in
Electrophoresis
Nanogap electrodes have attracted a lot of consideration as promising platform for molecular electronic and biomolecules detection. This is mainly for their higher aspect ratio, and because their electrical properties are easily accessed by current-voltage measurements. Nevertheless, application of standard current-voltages measurements used to cha...
Wang, Mengmeng Fang, Meng Zhu, Jianhui Feng, Huijuan Warner, Elisa Yi, Changhong Ji, Jun Gu, Xing Gao, Chunfang
Published in
Electrophoresis
Extensive efforts have been devoted to improve the diagnosis of extrahepatic cholangiocarcinoma (ECCA) due to its silent clinical character and lack of effective diagnostic biomarkers. Specific alterations in N-glycosylation of glycoproteins are considered a key component in cancer progression, which can serve as a distinct molecular signature for ...
Penny, William M Palmer, Christopher P
Published in
Electrophoresis
Styrene-maleic acid polymer-bound lipid bilayer nanodiscs have been investigated and characterized by electrokinetic chromatography. Linear solvation energy relationship analysis was employed to characterize the changes in solvation environment of nanodiscs of varied belt to lipid ratio, belt polymer chemistry and molecular weight, and lipid compos...
Sung, EunJi Kwon, Oh Kwang Lee, Jae-Mok Lee, Sangkyu
Published in
Electrophoresis
Prostate cancer is the leading type of cancer diagnosed, and the most frequent cause of worldwide male cancer-related deaths annually. The limitations of current prostate cancer screening tests demand the identification of novel biomarkers for the early diagnosis of prostate cancer bone metastasis. In the present study, we performed a proteomic ana...
Liu, Yue Hu, Jia Li, Yan Shang, Yun-Tao Wang, Jia-Qi Zhang, Ye Wang, Zhong-Liang
Published in
Electrophoresis
In this work, a CE method was developed to separate five anthraquinones: aloe-emodin, rhein, emodin, chrysophanol, and physcion. The CE method used a nano-sized metal organic framework MIL-101 (nMIL-101) as pseudostationary phase (PSP) and sorbent for dispersed particle extraction (DPE). The nMIL-101 was synthesized by microwave technique and was c...
Wang, Zhichao Ivory, Cornelius Minerick, Adrienne R
Published in
Electrophoresis
Isoelectric focusing (IEF) is a powerful tool for amphoteric protein separations because of high sensitivity, bio-compatibility, and reduced complexity compared to chromatography or mechanical separation techniques. IEF miniaturization is attractive because it enables rapid analysis, easier adaptation to point of care applications, and smaller samp...
