Lin, Chao-Chieh Yan, Jin Kapur, Meghan D Norris, Kristi L Hsieh, Cheng-Wei Huang, De Vitale, Nicolas Lim, Kah-Leong Guan, Ziqiang Wang, Xiao-Fan
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Published in
EMBO reports
Autophagy has emerged as the prime machinery for implementing organelle quality control. In the context of mitophagy, the ubiquitin E3 ligase Parkin tags impaired mitochondria with ubiquitin to activate autophagic degradation. Although ubiquitination is essential for mitophagy, it is unclear how ubiquitinated mitochondria activate autophagosome ass...
Hu, Liang Brichalli, Wyatt Li, Naren Chen, Shifan Cheng, Yaqing Liu, Qinfang Xiong, Yulan Yu, Jianzhong
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EMBO reports
The Hippo pathway is an evolutionarily conserved developmental pathway that controls organ size by integrating diverse regulatory inputs, including actomyosin-mediated cytoskeletal tension. Despite established connections between the actomyosin cytoskeleton and the Hippo pathway, the upstream regulation of actomyosin in the Hippo pathway is less de...
Baldwin, Tanya A Li, Yong Marsden, Autumn N Rinné, Susanne Garza-Carbajal, Anibal Schindler, Roland F R Zhang, Musi Garcia, Mia A Venna, Venugopal Reddy Decher, Niels
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EMBO reports
The establishment of macromolecular complexes by scaffolding proteins is key to the local production of cAMP by anchored adenylyl cyclase (AC) and the subsequent cAMP signaling necessary for cardiac functions. We identify a novel AC scaffold, the Popeye domain-containing (POPDC) protein. The POPDC family of proteins is important for cardiac pacemak...
Sadic, Mohammad Schneider, William M Katsara, Olga Medina, Gisselle N Fisher, Ashley Mogulothu, Aishwarya Yu, Yingpu Gu, Meigang de Los Santos, Teresa Schneider, Robert J
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EMBO reports
Co-opting host cell protein synthesis is a hallmark of many virus infections. In response, certain host defense proteins limit mRNA translation globally, albeit at the cost of the host cell's own protein synthesis. Here, we describe an interferon-stimulated helicase, DDX60, that decreases translation from viral internal ribosome entry sites (IRESs)...
Russ, Alina Wittmann, Sabine Tsukamoto, Yuta Herrmann, Alexandra Deutschmann, Janina Lagisquet, Justine Ensser, Armin Kato, Hiroki Gramberg, Thomas
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EMBO reports
Methylation of the mRNA 5' cap by cellular methyltransferases enables efficient translation and avoids recognition by innate immune factors. Coronaviruses encode viral 2'-O-methyltransferases to shield their RNA from host factors. Here, we generate recombinant SARS-CoV-2 harboring a catalytically inactive 2'-O-methyltransferase Nsp16, Nsp16mut, and...
Martens, Arne Hertens, Pieter Priem, Dario Rinotas, Vagelis Meletakos, Theodore Gennadi, Meropi Van Hove, Lisette Louagie, Els Coudenys, Julie De Muynck, Amélie
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EMBO reports
The anti-inflammatory protein A20 serves as a critical brake on NF-κB signaling and NF-κB-dependent inflammation. In humans, polymorphisms in or near the TNFAIP3/A20 gene have been associated with several inflammatory disorders, including rheumatoid arthritis (RA), and experimental studies in mice have demonstrated that myeloid-specific A20 deficie...
Kaufmann, Benedikt Leszczynska, Aleksandra Reca, Agustina Booshehri, Laela M Onyuru, Janset Tan, ZheHao Wree, Alexander Friess, Helmut Hartmann, Daniel Papouchado, Bettina
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EMBO reports
Sterile inflammation is a central element in liver diseases. The immune response following injurious stimuli involves hepatic infiltration of neutrophils and monocytes. Neutrophils are major effectors of liver inflammation, rapidly recruited to sites of inflammation, and can augment the recruitment of other leukocytes. The NLRP3 inflammasome has be...
Huang, Ting Jiang, Gaoyue Zhang, Yabin Lei, Yuqing Liu, Shiyan Li, Huihui Lu, Kefeng
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EMBO reports
Macroautophagy/autophagy is a conserved process in eukaryotic cells that mediates the degradation and recycling of intracellular substrates. Proteins encoded by autophagy-related (ATG) genes are essentially involved in the autophagy process and must be tightly regulated in response to various circumstances, such as nutrient-rich and starvation cond...
Moore, Rachel E Pop, Sînziana Alleyne, Caché Clarke, Jonathan D W
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EMBO reports
Our understanding of the cell behaviours and cytoskeletal requirements of axon formation is largely derived from in vitro models but how these relate to axon formation in vivo is not clear. In vitro, neurons progress through a well-defined multineurite stage to form an axon and both actin and microtubules cooperate to drive the first steps in neuri...
Chen, Lu Zhang, Xianwen Guo, Xuan Peng, Wenyu Zhu, Yibin Wang, Zhaoyang Yu, Xi Shi, Huicheng Li, Yuhan Zhang, Liming
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EMBO reports
Frequent turnover of dengue virus (DENV) clades is one of the major forces driving DENV persistence and prevalence. In this study, we assess the fitness advantage of nine stable substitutions within the envelope (E) protein of DENV serotypes. Two tandem neighboring substitutions, threonine to lysine at the 226th (T226K) and glycine to glutamic acid...