Published in Developmental cell
3D cell cultures, in particular organoids, are emerging models in the investigation of healthy or diseased tissues. Understanding the complex cellular sociology in organoids requires integration of imaging modalities across spatial and temporal scales. We present a multi-scale imaging approach that traverses millimeter-scale live-cell light microsc...
Published in Developmental Cell
Galupa et al. compared the potential of random sequences versus point mutations of existing enhancers to generate new patterns of expression during Drosophila melanogaster development. Their findings point to the activity of de novo elements as a primary source for phenotypic novelty in developmental enhancers’ evolution.
Published in Developmental Cell
Viegas et al. explore the role of cell-cycle-associated protein 1 (CAPRIN1) in early murine-differentiated ESCs, and they show that CAPRIN1 promotes the degradation of thousands of RNA transcripts and that it interacts with exoribonuclease XRN2. The authors define a mechanism whereby CAPRIN1 (via XRN2) causes RNA degradation to eliminate undesired ...
Published in Developmental Cell
Published in Developmental cell
Blood-vessel formation generates unique vascular patterns in each individual. The principles governing the apparent stochasticity of this process remain to be elucidated. Using mathematical methods, we find that the transition between two fundamental vascular morphogenetic programs-sprouting angiogenesis and vascular remodeling-is established by a ...
Published in Developmental cell
Microglia, the brain's resident macrophages, shape neural development and are key neuroimmune hubs in the pathological signatures of neurodevelopmental disorders. Despite the importance of microglia, their development has not been carefully examined in the human brain, and most of our knowledge derives from rodents. We aimed to address this gap in ...
Published in Developmental Cell
Continuous time-lapse imaging of developing mammalian organs is not yet possible. Dalmasso et al. propose a computer-based approach to recreate a continuous evolution in time and space from a set of 3D volumetric images using spherical harmonics. The result allows for a data-driven quantitative 4D description of limb and heart morphogenesis.
Published in Developmental Cell
Miguel-Escalada et al. show that pancreas agenesis mutations disrupt a lead enhancer that activates other PTF1A enhancers in early progenitors of the embryonic pancreas. This enables transient PTF1A expression in progenitors, which controls pancreas growth and creates epigenetic competence for subsequent endocrine differentiation.
Published in Developmental cell
Cells with latent stem ability can contribute to mammalian tissue regeneration after damage. Whether the central nervous system (CNS) harbors such cells remains controversial. Here, we report that DNGR-1 lineage tracing in mice identifies an ependymal cell subset, wherein resides latent regenerative potential. We demonstrate that DNGR-1-lineage-tra...
Published in Developmental Cell
Reactive oxygen species (ROS) are by-products of mitochondrial respiration. Kirova et al. find that mitochondrial ROS increase during the cell cycle and oxidize cyclin-dependent kinase 2 (CDK2) to regulate its interaction with KAP phosphatase. CDK2 oxidation promotes T-loop phosphorylation and the CDK2 activity needed for DNA replication and prolif...
