Correction to: Characteristics of apoptosis induction in human breast cancer cells treated with a ceramidase inhibitor
Published in Cytotechnology
Characteristics of apoptosis induction in human breast cancer cells treated with a ceramidase inhibitor
Published in Cytotechnology
Cancer is a complex disease with high mortality rates. Breast cancer is one of the most fatal diseases both for men and woman. Despite the positive developments on cancer treatment, a successful treatment agent/method has not been developed, yet. Recently, cancer research has been involved in sphingolipid metabolism. The key molecule here is cerami...
Production of interspecies somatic/pluripotent heterokaryons using polyethylene glycol (PEG) and selection by imaging fl...
Published in Cytotechnology
Fusion of somatic cells to embryonic stem cells induces reprogramming of the somatic nucleus and can be used to study the effect of trans-acting factors from the pluripotent cell over the differentiated nucleus. However, fusion only occurs in a small fraction of the cells exposed to fusogenic conditions, hence the need for a protocol that produces ...
Companion animals get close to the toxic aspects of antropogenic world: cytotoxicity of phthalates and bisphenol A on do...
Published in Cytotechnology
Phthalates, which are among the most abundant plasticizers, have detrimental effects on the reproductive system. Similar to human, dogs are prominently exposed to phthalates in daily routines at low concentrations; while toys, training devices and commercial dog foods are considered as the primary sources of exposure. This study aimed to reveal and...
Development of novel human in vitro vascularized adipose tissue model with functional macrophages.
Published in Cytotechnology
Inflammation has been proven significant factor in development of type 2 diabetes. So far, most of the adipose tissue related research has been performed in animals, mainly rodent models. The relevance of translation of animal results to humans is questionable. However, in vitro model with relevant human cell source, such as human adipose tissue st...
Vimentin plays an important role in the promotion of breast cancer cell migration and invasion by leucine aminopeptidase...
...Published in Cytotechnology
Breast cancer is a common type of cancer in females. Our previous studies indicated that leucine aminopeptidase 3 (LAP3) promotes migration and invasion of breast cancer cells. Vimentin is a mesenchymal marker, and its upregulation represents the promotion of epithelial-mesenchymal transition. In this study, we found that LAP3 and vimentin were hig...
Expansion of mouse primitive hematopoietic cells in three-dimensional cultures on chemically fixed stromal cell layers.
Published in Cytotechnology
To establish a practical and convenient method to expand hematopoietic cells (HCs), we applied chemically-fixed stromal cell layers formed within three-dimensional (3D) scaffolds to feeder of HC cultures. The HCs were expanded using two successive cultures. First, stromal cells were cultured within porous polymer scaffolds and formed tissue-enginee...
Generation of GABAergic striatal neurons by a novel iPSC differentiation protocol enabling scalability and cryopreservat...
...Published in Cytotechnology
Cell models are promising tools for studying hereditary human neurodegenerative diseases. Neuronal derivatives of pluripotent stem cells provide the opportunity to investigate different stages of the neurodegeneration process. Therefore, easy and large-scale production of relevant cell types is a crucial barrier to overcome. In this work, we presen...
Automated in-process characterization and selection of cell-clones for quality and efficient cell manufacturing.
Published in Cytotechnology
Delivery of safe, effective and reliable cellular therapies, whether based on mesenchymal stromal cells (MSCs) or induced pluripotent stem cells (iPSCs), demand standardization of cell culture protocols. There is a need to develop automation platform that enables the users to generate culture expanded human cell populations that improves the qualit...
Establishment and characterization of a PCOS and a normal human granulosa cell line.
Published in Cytotechnology
Oocyte maturation is an important phase in fertility and any disorder in this process could lead to infertility. The most common disorder during folliculogenesis is polycystic ovary syndrome (PCOS). Due to the secretive activity of granulosa cells (GCs), they play a vital role in folliculogenesis. Although scientists use various cellular and molecu...
