Faghih, Hossein Javeri, Arash Taha, Masoumeh Fakhr
Published in
Cytotechnology
Adipose tissue-derived stem cells (ADSCs) are capable of multipotential differentiation and express several angiogenic, anti-apoptotic and immunomodulatory markers. These features make adipose tissue as a promising source of stem cells for regenerative medicine. However, for efficient translational use, culture-induced changes in the gene expressio...
Tonello, Jane Marie Kawashima, Saori Sato, Kazuki Kawabe, Yoshinori Ito, Akira Kamihira, Masamichi
Published in
Cytotechnology
Hepatoma cells are a candidate cell source for bio-artificial livers. However, they exhibit reduced liver functions compared with primary hepatocytes. In our previous study, genetically engineered mouse hepatoma cells were created by transduction with vectors mediating inducible overexpression of eight liver-enriched transcription factors. Upon the...
Drwal, Eliza Rak, Agnieszka Gregoraszczuk, Ewa
Published in
Cytotechnology
Monocultures of different placental cells are used for many physiological and toxicological studies; however, they are not a true reflection of the interaction between placenta and fetus. To develop the most appropriate model to study endocrine and metabolic properties of fetoplacental unit we used three co-culture models of placental cells nonfuso...
Wang, Min Gui, Chunyi Qiu, Shenglong Tang, Jingdong Peng, Zhihai
Published in
Cytotechnology
Clinical evidence indicates that high periostin expression correlates with aggressive phenotype in thyroid carcinoma. However, the biological roles of periostin in thyroid carcinoma development and progression are still unclear. In this study, we explored the effects of periostin silencing on thyroid carcinoma cell growth, invasion, and tumorigenes...
Xiang, Yang Zhao, Ming-Ming Sun, Sujiao Guo, Xiao-Long Wang, Qiquan Li, Sheng-An Lee, Wen-Hui Zhang, Yun
Published in
Cytotechnology
Dimethyl sulfoxide (DMSO) is widely used in the laboratory and in clinical situations because it is soluble in both aqueous and organic media and can be used to treat many types of diseases. Thus, it is meaningful to assess the comprehensive and in-depth biological activities of DMSO. Here, we showed that a high concentration of DMSO induced pro-in...
Zhang, Heng Jiao, Yunping Shi, Chunyang Song, Xiao Chang, Ying Ren, Yong Shi, Xiaolin
Published in
Cytotechnology
Berbamine has been shown to exhibit anti-cancer activities in various types of cancers. The effects of berbamine on colorectal colon cancer (CRC) have not been examined, and the present study aimed to investigate the anti-cancer effects of berbamine in CRC and explore its underlying molecular mechanisms. The effect of berbamine on the CRC cells was...
Eitoku, Masamitsu Kato, Hidemasa Suganuma, Narufumi Kiyosawa, Hidenori
Published in
Cytotechnology
Understanding gene expression in the brain requires allele-specific transcriptome analysis because of the presence of neuron-specific imprinted genes, which are expressed in a neuron-specific and parent-of-origin-specific manner. Ube3a is a neuron-specific imprinted gene with an expression pattern that changes from biallelic to maternal only (Ube3a...
Forcato, D O Fili, A E Alustiza, F E Lázaro Martínez, J M Bongiovanni Abel, S Olmos Nicotra, M F Alessio, A P Rodríguez, N Barbero, C Bosch, P
...
Published in
Cytotechnology
The development of efficient transfection protocols for livestock cells is crucial for implementation of cell-based transgenic methods to produce genetically modified animals. We synthetized fully deacylated linear 22, 87 and 217 kDa polyethylenimine (PEI) nanoparticles and compared their transfection efficiency and cytotoxicity to commercial branc...
Borges, Alana Azevedo Lima, Gabriela Liberalino de Queiroz Neta, Luiza Bento Santos, Maria Valéria de Oliveira de Oliveira, Moacir Franco Silva, Alexandre Rodrigues Pereira, Alexsandra Fernandes
Published in
Cytotechnology
Cryopreservation of somatic tissue can be applied in biodiversity conservation, especially for wild species as collared peccary. We aimed to evaluate the effect of vitrification techniques of ear tissue of collared peccary [direct vitrification in cryovials (DVC) or solid-surface vitrification (SSV)] on the layers of epidermis and dermis by convent...
Sharath Babu, G R Ilaiyaraja, N Khanum, Farhath Anand, T
Published in
Cytotechnology
The mycotoxin citrinin, is produced by several species of Penicillium, Aspergillus and Monascus, and is capable of inducing cytotoxicity, oxidative stress and apoptosis. The aim of the present study was to investigate the effect of citrinin in mouse skeletal muscle cells (C2C12) and to overcome the cellular adverse effects by supplementing green te...
