Gaihre, Yuba Raj Iwamoto, Akira Oogai, Shigeki Hamajima, Hiroshi Tsuge, Keisuke Nagata, Yasuo Yanagita, Teruyoshi
Published in
Cytotechnology
Perilla frutescens (L.) Britton var. frutescens (egoma in Japan) is a traditional oilseed that has several varieties with different photoperiod responses. Although egoma pomace, industrial waste produced during oil extraction, is a rich source of macro- and micro-nutrients such as protein, fiber, minerals, and polyphenols, it has not yet been used ...
Baba, Akira Suwada, Takuya Muta, Shigeru Kuhara, Satoru Tashiro, Kosuke
Published in
Cytotechnology
The epigenetic regulatory system significant influences the fate determination of cells during developmental processes. Prdm12 is a transcriptional regulator that modulates gene expression epigenetically. The Prdm12 gene has been shown to be expressed in neural tissues, specifically during development, but its detailed function is not fully underst...
Iwamoto, Akira Yamauchi, Ryoko Oogai, Shigeki Tsuruta, Yumi Keisuke, Tsuge Nagata, Yasuo Yanagita, Teruyoshi
Published in
Cytotechnology
Lotus root is a traditional food ingredient used primarily in Asia and is rich in polyphenols. To determine its potential use in antiphotoaging, polyphenols were extracted from lotus root with 50% ethanol, and the activity of matrix metalloproteinase (MMP) was measured in dermal cells treated with ultraviolet A (UVA). UVA exposure increased the gen...
Li, Binglong An, Di Zhu, Shasha
Published in
Cytotechnology
PBX1 expression has been found to be significantly reduced in nigrostriatal neurons of PD patients, but the effect of PBX1 on ROS and apoptosis in nigrostriatal dopamine neurons is not yet known. This paper aimed to explore whether PBX1 could be involved in the development of PD. The construction of the in vitro PD model was followed by the determi...
Xiao, Jingwen Jiang, Rongrong Yin, Weiwei Zhang, Ye Cao, Peipei Li, Jianxin Gong, Yurong Ding, Xiaolin Shi, Suping Hao, Jie
...
Published in
Cytotechnology
Dental pulp stem cells (DPSCs) are well known for their capable of both self-renewal and multilineage differentiation. Dental tissue diseases, include caries, are often accompanied by inflammatory microenvironment, and muramyl dipeptide (MDP) is involved in the inflammatory stimuli to influence the differentiation of DPSCs. Nucleotide-binding oligo...
Kardani, Kimia Milani, Alireza Bolhassani, Azam
Published in
Cytotechnology
Physical methods are widely utilized to deliver nucleic acids into cells such as electro-transfection or heat shock. An efficient gene electro-transfection requires the best conditions including voltage, the pulse length or number, buffer, incubation time and DNA form. In this study, the delivery of pEGFP-N1 vector into two adherent cell lines (HEK...
Ersöz, Nur Şebnem Adan, Aysun
Published in
Cytotechnology
Treatment of FMS-like tyrosine kinase 3 (FLT3)-internal tandem duplication (ITD) AML is restricted due to toxicity, drug resistance and relapse eventhough targeted therapies are clinically available. Resveratrol with its multi-targeted nature is a promising chemopreventive remaining limitedly studied in FLT3-ITD AML regarding to ceramide metabolism...
Yang, Zhengyuan Ye, Xiajun Zhang, Yujie Huang, Yiteng Chen, Jian Zeng, Yunzhu Chen, Jiongyu
Published in
Cytotechnology
Nasopharyngeal carcinoma (NPC) is a malignant tumor with a poor prognosis. Studies have shown that esophageal carcinoma related gene 4 (ECRG4) is hypermethylated and significantly downregulated in NPC tissues. However, the role of ECRG4 in NPC, and in particular the underlying molecular mechanism, is largely unclear. In this study, using immunohist...
Zhang, Jia Liu, Zhou Liu, Lingpeng Huang, Mingwen Huang, Yong
Published in
Cytotechnology
T helper cell 22 are abundant in Hepatitis B Virus-related hepatocellular carcinoma tissue, and the main cytokine interleukin 22 produced by Th22 cells is closely related to the initiation and development of HCC. Understanding the role of Th22/IL-22 in the progression of HBV-related HCC will facilitate new therapeutic development. Th22 cells were i...
Fujiki, Tsukasa Matsumoto, Shin-Ei Kishihara, Kenji Katakura, Yoshinori
Published in
Cytotechnology
This study aimed to investigate the changes in B cell functional decline and antigen sensitization with aging using two Epstein Barr virus (EBV)-immortalized human B cell lines, one from a 22-year-old man (EBV-B young) and the other from a 65-year-old man (EBV-B old). The activity of senescence-associated β-galactosidase, a marker of cellular senes...
