Bandyopadhyay, Shovik Fowles, Jared S Yu, Liyang Fisher, Daniel A C Oh, Stephen T
Published in
Cytometry. Part B, Clinical cytometry
Background: Mass cytometry (CyTOF) is a powerful tool for analyzing cellular networks at the single cell level. Due to the high-dimensional nature of this approach, analysis algorithms have been developed to visualize and interpret mass cytometry data. In this study, we applied these approaches to a cohort of patients with secondary acute myeloid l...
Wheeler, M White, G Brockie, S Dickson, M Weinkove, R
Published in
Cytometry. Part B, Clinical cytometry
Bone marrow (BM) aspirate analysis by flow cytometry (FC) is a key hematopathologic technique but dry taps hinder diagnosis. We describe the utility and limitations of a method of mechanically disaggregating BM trephine biopsies for FC. Trephine biopsies mechanically disaggregated for FC between 2010 and 2016 were reviewed. We recorded cell yield, ...
Simões, Catarina Silva, Isabel Carvalho, Anabela Silva, Sandra Santos, Susana Marques, Gilberto Ribeiro, André Roque, Adriana Carda, José Sarmento-Ribeiro, A B
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Published in
Cytometry. Part B, Clinical cytometry
Vδ1+ T cells, a subset of γδ T cells, are responsible for innate-like immune responses. Recently, an anti-tumor function mediated by MHC-unrestricted recognition of lipid and stress molecules, has also been described in these cells. This study aimed to quantify and phenotypically characterize circulating Vδ1+ T cells in B cell Chronic Lymphocytic L...
Gardikas, Nikolaos Vikentiou, Myrofora Konsta, Evgenia Kontos, Christos K Papageorgiou, Sotirios G Spathis, Aris Bazani, Efthimia Bouchla, Anthi Kapsimali, Violetta Psarra, Katherina
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Published in
Cytometry. Part B, Clinical cytometry
Myelodysplastic syndromes (MDS) are clonal hematopoietic stem cell disorders with unknown aetiology. Multiparameter flow cytometry (MFC) is being evaluated for the diagnosis and prognosis of MDS. In the present study, five-color MFC was performed on bone marrow aspirates of 50 untreated patients, newly diagnosed with MDS and 27 age matched control ...
Feng, Ru Bhatt, Vijaya Raj Fu, Kai Pirruccello, Samuel Yuan, Ji
Published in
Cytometry. Part B, Clinical cytometry
The purpose of this study was to determine whether immunophenotypic profiles detected by flow cytometry are useful in differentiating chronic myelomonocytic leukemia (CMML) from reactive monocytosis, and between CMML subtypes. Eight-color flow cytometry was used to immunophenotype blasts, monocytes, and granulocytes in the bone marrow of 34 patient...
Marcelletti, John F Sikic, Branimir I Cripe, Larry D Paietta, Elisabeth
Published in
Cytometry. Part B, Clinical cytometry
Multidrug resistance (MDR) transporter proteins such as P-glycoprotein (P-gp) efflux a variety of chemotherapeutic drugs from acute myeloid leukemia (AML) blasts leading to clinical drug resistance. This study examined heterogeneity of MDR functional efflux by AML blasts using two flow cytometry bioassays. Bone marrow specimens (N = 50) from elderl...
Garcia-Prat, Marina Álvarez-Sierra, Daniel Aguiló-Cucurull, Aina Salgado-Perandrés, Sandra Briongos-Sebastian, Sara Franco-Jarava, Clara Martin-Nalda, Andrea Colobran, Roger Montserrat, Isabel Hernández-González, Manuel
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Published in
Cytometry. Part B, Clinical cytometry
For the accurate diagnosis of immunodeficiencies is crucial to compare patients' immunology laboratory values with age-sex matched controls, yet there is a paucity of normal values for most populations. To define appropriate reference values of extended lymphocyte subpopulations and T-cell receptor excision circle (TRECs) levels in healthy pediatri...
Zeng, Yang Hiti, Alan Moranville, Sherry Vicent, Gloria Chavira, Sylvia de Arruda Indig, Monika Graminske, Sharon Boerner, Amanda Schmidt, Anna Oreizy, Farzad
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Published in
Cytometry. Part B, Clinical cytometry
The BD FACSVia™ system is a novel flow cytometer with improved workflow efficiencies. To evaluate the HLA-B27 application developed on the BD FACSVia system utilizing the BD™ HLA-B27 kit, we conducted a concordance study at three centers to compare with the BD FACSCalibur™ system. Prepared donor samples (n = 594) were analyzed on both the BD FACSVi...
Vazquez, Romain Roussel, Mikaël Badaoui, Bouchra Freynet, Nicolas Tarfi, Sihem Solary, Eric Selimoglu-Buet, Dorothée Wagner-Ballon, Orianne
Background: Accumulation of classical monocytes CD14++CD16– (also called MO1) ≥ 94% can accurately distinguish chronic myelomonocytic leukemia (CMML) from reactive monocytosis. The HematoFlow™ solution, able to quantify CD16 negative monocytes, could be a useful tool to manage monocytosis which remains a common issue in routine laboratories.Methods...
Carulli, Giovanni Caracciolo, Francesco Sammuri, Paola Domenichini, Cristiana Tarasco, Angela Rousseau, Martina Ottaviano, Virginia Petrini, Mario
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Cytometry. Part B, Clinical cytometry
