Eidhof, Harrie H M Gratama, Jan W Mulder, A H Leontine
Published in
Cytometry. Part B, Clinical cytometry
Flowcytometric analysis of lymphocytes and their subpopulations in bronchoalveolar lavages (BAL) can support the diagnosis of interstitial lung diseases. This analysis should be done within 4 hr after lavage due to rapid cell deterioration. We tested three methods in order to stabilize for at least 28 days the BAL cell populations to allow delayed ...
Hu, Zhihong Wang, Wei Thakral, Beenu Chen, Zhining Estrov, Zeev Bueso-Ramos, Carlos E Verstovsek, Srdan Medeiros, L Jeffrey Wang, Sa A
Published in
Cytometry. Part B, Clinical cytometry
Lymphocytic variant of hypereosinophilic syndrome (L-HES) is a subtype of HES driven by cytokines produced by clonal T-cells. Due to the rarity of its occurrence and challenges in diagnosis, this subtype of HES is under recognized. We report seven patients with L-HES, diagnosed from a group of 136 patients who were referred to our institution for t...
Bagwell, Charles Bruce
Published in
Cytometry. Part B, Clinical cytometry
Berg, Holly Otteson, Gregory E Corley, Heidi Shi, Min Horna, Pedro Jevremovic, Dragan Olteanu, Horatiu
Published in
Cytometry. Part B, Clinical cytometry
Flow cytometric detection of T-cell clonality is challenging. The current available methodology for T-cell receptor (TCR) Vβ repertoire evaluation is a complex assay and has limited sensitivity especially for detecting low levels of disease. Therefore, there is an unmet need for a reliable, simple, and rapid assay to identify T-cell clonality. The ...
Beucke, Nicola Wistuba-Hamprecht, Kilian
Published in
Cytometry. Part B, Clinical cytometry
Yu, Jianlin Tan, Liming Wu, Qiong Rao, Yanfei Ao, Jianyun Yang, Weiming Zou, Bei Chen, Juanjuan
Published in
Cytometry. Part B, Clinical cytometry
Multiple myeloma (MM) is a common malignant disease of the blood system, caused by the neoplastic proliferation of plasma cells that accumulate in bone marrow (BM). Here, we report a case of MM patient with CD138 marker changed from positive to negative. BM and peripheral blood samples from a 48-year-old patient with MM were examined and analyzed b...
Hill, Ainsleigh J Zhang, Chaoran Kusakabe, Manabu Gowing, Kevin Wang, Xuehai Brinkman, Ryan R Weng, Andrew P Craig, Jeffrey W
Published in
Cytometry. Part B, Clinical cytometry
Collins, Katrina Cardinali, Jolene L Mnayer, Laila O DiGiuseppe, Joseph A
Published in
Cytometry. Part B, Clinical cytometry
CD49f (integrin α6) is a useful marker for minimal residual disease (MRD) detection in B lymphoblastic leukemia and has recently been suggested to mediate infiltration of the central nervous system by leukemic B lymphoblasts. However, data regarding expression of CD49f protein in B lymphoblastic leukemia are limited, and whether CD49f protein expre...
Craig, Fiona E
Published in
Cytometry. Part B, Clinical cytometry
Horna, Pedro Wang, Sa A Wolniak, Kristy L Psarra, Katherina Almeida, Julia Illingworth, Andrea J Johansson, Ulrika Craig, Fiona E Torres, Richard
Published in
Cytometry. Part B, Clinical cytometry
A peripheral blood flow cytometric assay for Sézary syndrome (SS) or circulating mycosis fungoides (MF) cells must be able to reliably identify, characterize, and enumerate T-cells with an immunophenotype that differs from non-neoplastic T-cells. Although it is also important to distinguish SS and MF from other subtypes of T-cell neoplasm, this usu...
