Kowarsch, Florian Maurer-Granofszky, Margarita Weijler, Lisa Wödlinger, Matthias Reiter, Michael Schumich, Angela Feuerstein, Tamar Sala, Simona Nováková, Michaela Faggin, Giovanni
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Published in
Cytometry. Part A : the journal of the International Society for Analytical Cytology
T-lineage acute lymphoblastic leukemia (T-ALL) accounts for about 15% of pediatric and about 25% of adult ALL cases. Minimal/measurable residual disease (MRD) assessed by flow cytometry (FCM) is an important prognostic indicator for risk stratification. In order to assess the MRD a limited number of antibodies directed against the most discriminati...
Rodríguez-Martínez, Eder Alejandro Rivas, Cindy U Ayala, María E Blanco-Rodríguez, Rodolfo Juarez, Nancy Hernandez-Vargas, Esteban A Aragón, Andrés
Published in
Cytometry. Part A : the journal of the International Society for Analytical Cytology
The identification of kinematic subpopulations is of paramount importance to understanding the biological nature of the sperm heterogeneity. Nowadays, the data of motility parameters obtained by a computer-assisted sperm analysis (CASA) system has been used as input to distinct algorithms to identify kinematic subpopulations. In contrast, the image...
Yao, Yifan He, Li Mei, Liye Weng, Yueyun Huang, Jin Wei, Shubin Li, Rubing Tian, Sheng Liu, Pan Ruan, Xiaolan
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Published in
Cytometry. Part A : the journal of the International Society for Analytical Cytology
Essential thrombocythemia (ET) is an uncommon situation in which the body produces too many platelets. This can cause blood clots anywhere in the body and results in various symptoms and even strokes or heart attacks. Removing excessive platelets using acoustofluidic methods receives extensive attention due to their high efficiency and high yield. ...
Stevens, Chad R Atkuri, Kondala Menard, Daniele L King, Lindsay E Neubert, Hendrik Goihberg, Polina
Published in
Cytometry. Part A : the journal of the International Society for Analytical Cytology
Characterization of target abundance on cells has broad translational applications. Among the approaches for assessing membrane target expression is quantification of the number of target-specific antibody (Ab) bound per cell (ABC). ABC determination on relevant cell subsets in complex and limited biological samples necessitates multidimensional im...
van Wolfswinkel, Marjolein van Meijgaarden, Krista E Ottenhoff, Tom H M Niewold, Paula Joosten, Simone A
Published in
Cytometry. Part A : the journal of the International Society for Analytical Cytology
Characterization of immune cells is essential to advance our understanding of immunology and flow cytometry is an important tool in this context. Addressing both cellular phenotype and antigen-specific functional responses of the same cells is valuable to achieve a more integrated understanding of immune cell behavior and maximizes information obta...
Batta, Ágnes Hajdu, Tímea Nagy, Peter
Published in
Cytometry. Part A : the journal of the International Society for Analytical Cytology
Förster resonance energy transfer (FRET) is a radiationless interaction between a donor and an acceptor whose distance dependence makes it a sensitive tool for studying the oligomerization and the structure of proteins. When FRET is determined by measuring the sensitized emission of the acceptor, a parameter characterizing the ratio of detection ef...
Patrick, Ellis Canete, Nicolas P Iyengar, Sourish S Harman, Andrew N Sutherland, Greg T Yang, Pengyi
Published in
Cytometry. Part A : the journal of the International Society for Analytical Cytology
Highly multiplexed in situ imaging cytometry assays have made it possible to study the spatial organization of numerous cell types simultaneously. We have addressed the challenge of quantifying complex multi-cellular relationships by proposing a statistical method which clusters local indicators of spatial association. Our approach successfully ide...
Bacon, Bezalel Repin, Mikhail Shuryak, Igor Wu, Hui-Chen Santella, Regina M Terry, Mary Beth Brenner, David J Turner, Helen C
Published in
Cytometry. Part A : the journal of the International Society for Analytical Cytology
Peripheral blood mononuclear cells (PBMCs) are a useful model for biochemical assays, particularly for etiological studies. We describe here a method for measuring DNA repair capacity (DRC) in archival cryogenically preserved PBMCs. To model DRC, we measured γ-H2AX repair kinetics in thawed PBMCs after irradiation with 3 Gy gamma rays. Time-depende...
Kinegawa, Ryo Gala de Pablo, Julia Wang, Yi Hiramatsu, Kotaro Goda, Keisuke
Published in
Cytometry. Part A : the journal of the International Society for Analytical Cytology
Label-free imaging flow cytometry is a powerful tool for biological and medical research as it overcomes technical challenges in conventional fluorescence-based imaging flow cytometry that predominantly relies on fluorescent labeling. To date, two distinct types of label-free imaging flow cytometry have been developed, namely optofluidic time-stret...
Maag, Abdul-Habib Swanton, Helen Kull, Miriam Vegi, Naidu M Feuring, Michaela
Published in
Cytometry. Part A : the journal of the International Society for Analytical Cytology
Acute erythroid leukemia (AEL) is a disease continuum between Myelodysplastic syndrome (MDS) and Acute myeloid leukemia (AML) with the cellular hallmark of uncontrolled proliferation and impaired differentiation of erythroid progenitor cells. First described by Giovanni di Guglielmo in 1917 AEL accounts for less than 5% of all de novo AML cases. Th...
