Published in Cryo letters
Worldwide women are increasingly facing the issue of delayed child-bearing and fertility decline. Oocyte cryopreservation provides an option for fertility preservation, especially for women with diseases and other special needs to conceive babies later. In this review we examine the effect of oocyte cryopreservation on early development of human em...
Published in Cryo letters
Cryopreservation is an effective tool for the preservation of live biological materials. This study examined the suitability of cryopreservation protocols and the effectiveness of ultrasound for silver carp embryos. Embryos at three developmental stages were exposed to 10, 15, 20, and 25% of five cryoprotectants (CPAs), namely propylene glycol (PG)...
Published in Cryo letters
Cryopreservation can induce cellular, genomic, and epigenetic abnormalities. To analyse the impact of ovarian vitrification on follicular development and its epigenetic effect on promoter methylation of Inhba and Inhbb in granulosa cells. Mouse ovaries were divided into control, toxicity, and vitrified groups. The growth and development of follicle...
Published in Cryo letters
The replacement of egg yolk with alternative plant-derived soybean lecithin is gaining interest in both animal and human sperm cryopreservation owing to biosecurity issues with egg yolk based extenders. To evaluate the comparative effect of egg yolk and soyabean lecithin based extenders on the quality of cryopreserved crossbred ram semen. Pooled ej...
Published in Cryo letters
Teramnus labialis (L.f.) Spreng is a potentially important legume species, and can be used as an animal feed and to enhance soil physicochemical characteristics. Despite the biological and agricultural importance, the low availability of seeds, their small size and the low percentage germination limit their large-scale use by farmers. We previously...
Published in Cryo letters
Cryopreservation is a reliable and economical method for the long-term ex situ conservation of valuable genetic resources. The present study focuses on establishing novel regeneration strategies and on assessing various cryogenic methods using nodal explants/shoot apices and on developing in vitro technologies for germplasm conservation of Dioscore...
Published in Cryo letters
Cryopreservation of sturgeon sperm can be successful, but there can be a decrease in sperm viability and the reasons are not clear. To investigate variations in the acrosin activity and the DNA integrity of Acipenser gueldenstaedtii semen during cryopreservation at -196ºC. Fish semen samples were randomly divided into three groups: [1] fresh contro...
Published in Cryo letters
The role of oxidative stress during cryoprotectant treatment has received little attention. OBJECTİVE: To assess the effects of different cryoprotectants and discover relationships between cryodamage and oxidative stress parameters on Awassi ram sperm. The sperm samples diluted with Salamon's tris-citrate (TRIS) containing 20% centrifuged egg yolk ...
Published in Cryo letters
The cryopreservation and recovery of epididymis tail sperm is an important biotechnology dependent on the composition of the freezing medium. To evaluate the effect of melatonin, added to commercial freezing medium extender, on the kinetics and viability of bovine epididymis tail sperm. Five routines were performed, each consisting of eight epididy...
Published in Cryo letters
Local fat accumulation is a health risk and this has raised interest in the development of aesthetic treatments, such as cryo-radiofrequency (CRF). To evaluate the consequences of CRF in adipose tissue remodeling in a model system. Lean and high-fat diet-induced obese mice were assessed 7 days after one CRF application; and lean mice were assessed ...
