Corrigendum: Niche Cells and Signals that Regulate Lung Alveolar Stem Cells In Vivo.
Published in Cold Spring Harbor perspectives in biology
Recognition and Degradation of Mislocalized Proteins in Health and Disease.
Published in Cold Spring Harbor perspectives in biology
A defining feature of eukaryotic cells is the segregation of complex biochemical processes among different intracellular compartments. The protein targeting, translocation, and trafficking pathways that sustain compartmentalization must recognize a diverse range of clients via degenerate signals. This recognition is imperfect, resulting in polypept...
Engineering Disease-Resistant Cassava.
Published in Cold Spring Harbor perspectives in biology
Manihot esculenta Crantz (cassava) is a food crop originating from South America grown primarily for its starchy storage roots. Today, cassava is grown in the tropics of South America, Africa, and Asia with an estimated 800 million people relying on it as a staple source of calories. In parts of sub-Saharan Africa, cassava is particularly crucial f...
Chaperone Interactions at the Ribosome.
Published in Cold Spring Harbor perspectives in biology
The continuous refreshment of the proteome is critical to maintain protein homeostasis and to adapt cells to changing conditions. Thus, de novo protein biogenesis by ribosomes is vitally important to every cellular system. This process is delicate and error-prone and requires, besides cytosolic chaperones, the guidance by a specialized set of molec...
The Role of Dwarfing Traits in Historical and Modern Agriculture with a Focus on Rice.
Published in Cold Spring Harbor perspectives in biology
Semidwarf stature is a valuable agronomic trait in grain crops that reduces lodging and increases harvest index. A fundamental advance during the 1960s Green Revolution was the introduction of semidwarf cultivars of rice and wheat. Essentially, all semidwarf varieties of rice under cultivation today owe their diminished stature to a specific null m...
Structural Insights into Nuclear pre-mRNA Splicing in Higher Eukaryotes.
Published in Cold Spring Harbor perspectives in biology
The spliceosome is a highly complex, dynamic ribonucleoprotein molecular machine that undergoes numerous structural and compositional rearrangements that lead to the formation of its active site. Recent advances in cyroelectron microscopy (cryo-EM) have provided a plethora of near-atomic structural information about the inner workings of the splice...
Principles and Practices of Hybridization Capture Experiments to Study Long Noncoding RNAs That Act on Chromatin.
Published in Cold Spring Harbor perspectives in biology
The diverse roles of cellular RNAs can be studied by purifying RNAs of interest together with the biomolecules they bind. Biotinylated antisense oligonucleotides that hybridize specifically to the RNA of interest provide a general approach to develop affinity reagents for these experiments. Such oligonucleotides can be used to enrich endogenous RNA...
Ca2+ Signaling and Regeneration.
Published in Cold Spring Harbor perspectives in biology
Regeneration is the process by which lost or damaged tissue is replaced in adult organisms. Some organisms exhibit robust regenerative capabilities, while others, including humans, do not. Understanding the molecular principles governing the regenerative malleability of different organisms is of fundamental biological interest. Further, this proble...
The Nuclear and DNA-Associated Molecular Chaperone Network.
Published in Cold Spring Harbor perspectives in biology
Maintenance of a healthy and functional proteome in all cellular compartments is critical to cell and organismal homeostasis. Yet, our understanding of the proteostasis process within the nucleus is limited. Here, we discuss the identified roles of the major molecular chaperones Hsp90, Hsp70, and Hsp60 with client proteins working in diverse DNA-as...
Many Facets of Dynamic Plasticity in Plants.
Published in Cold Spring Harbor perspectives in biology
The evolutionary processes that transitioned plants to land-based habitats also incorporated a multiplicity of strategies to enhance resilience to the greater environmental variation encountered on land. The sensing of light, its quality, quantity, and duration, is central to plant survival and, as such, serves as a central network hub. Similarly, ...
