The importance of the methodology and sample matrix when interpreting chromogranin A results
Published in Clinical Chemistry and Laboratory Medicine (CCLM)
Collection, transport and storage procedures for blood culture specimens in adult patients: recommendations from a board...
Published in Clinical Chemistry and Laboratory Medicine (CCLM)
Bloodstream infections (BSIs) remain a potentially life-threatening condition. The gold standard for the diagnosis of BSI is still blood cultures (BCs), and the diagnostic yield depends on clinical and technical factors that have an impact on collection and transportation. Hence, monitoring of the entire pre-analytical process from blood collection...
Blood biomarkers in neurology: “a call to arms” for laboratory professionals
Published in Clinical Chemistry and Laboratory Medicine (CCLM)
Analytical and clinical validation of a novel amplicon-based NGS assay for the evaluation of circulating tumor DNA in me...
...Published in Clinical Chemistry and Laboratory Medicine (CCLM)
Background Evaluating the tumor RAS/BRAF status is important for treatment selection and prognosis assessment in metastatic colorectal cancer (mCRC) patients. Correction of artifacts from library preparation and sequencing is essential for accurately analyzing circulating tumor DNA (ctDNA) mutations. Here, we assessed the analytical and clinical pe...
Diagnostic and prognostic value of the D-dimer test in emergency department patients: secondary analysis of an observati...
Published in Clinical Chemistry and Laboratory Medicine (CCLM)
Background D-dimer measurement improves the rule-out of thromboembolic disease. However, little is known about the risk of false positive results for the diagnosis of thromboembolic disease and its prognostic value. Herein, we investigated factors influencing the accuracy of D-dimer and its prognostic value in a large cohort of emergency department...
Elecsys® and Kryptor immunoassays for the measurement of sFlt-1 and PlGF to aid preeclampsia diagnosis: are they compara...
Published in Clinical Chemistry and Laboratory Medicine (CCLM)
Background For pregnant women with suspected preeclampsia, the soluble fms-like tyrosine-kinase 1 (sFlt-1)/placental growth factor (PlGF) ratio is a biomarker to aid diagnosis. We performed method comparisons between Elecsys® and Kryptor sFlt-1 and PlGF immunoassays and assessed the diagnostic performance for preeclampsia. Methods Serum samples fro...
Only monospecific anti-DFS70 antibodies aid in the exclusion of antinuclear antibody associated rheumatic diseases: an I...
...Published in Clinical Chemistry and Laboratory Medicine (CCLM)
Background The dense fine speckled (DFS) is one of the most common patterns that can be observed as a result of the anti-nuclear antibodies (ANA) test on HEp-2 cells and is mostly caused by antibodies to DFS70 as the main antigenic target. As was recently demonstrated, isolated anti-DFS70 positivity can be used as an aid in the exclusion of ANA ass...
Preliminary evaluation of a new flow cytometry method for the routine hematology workflow
Published in Clinical Chemistry and Laboratory Medicine (CCLM)
Background In a generalist laboratory, the integration of the data obtained from hematology analyzers (HAs) with those from multiparametric flow cytometry (FMC) could increase the specificity and sensitivity of first level screening to identify the pathological samples. The aim of this study was to perform a preliminary evaluation of a new simple h...
Handling of hemolyzed serum samples in clinical chemistry laboratories: the Nordic hemolysis project
Published in Clinical Chemistry and Laboratory Medicine (CCLM)
Background Some clinical chemistry measurement methods are vulnerable to interference if hemolyzed serum samples are used. The aims of this study were: (1) to obtain updated information about how hemolysis affects clinical chemistry test results on different instrument platforms used in Nordic laboratories, and (2) to obtain data on how test result...
Clinical biomarker innovation: when is it worthwhile?
Published in Clinical Chemistry and Laboratory Medicine (CCLM)
Background Choosing which biomarker tests to select for further research and development is not only a matter of diagnostic accuracy, but also of the clinical and monetary benefits downstream. Early health economic modeling provides tools to assess the potential effects of biomarker innovation and support decision-making. Methods We applied early h...
