Chung, Chyi Wei Kaminski Schierle, Gabriele S
Published in
Chembiochem : a European journal of chemical biology
Temperature is a fundamental physical parameter that influences biological processes in living cells. Hence, intracellular temperature mapping can be used to derive useful information reflective of thermodynamic properties and cellular behaviour. Herein, existing publications on different thermometry systems, focusing on those that employ fluoresce...
Freund, Christian Schwarzer, Dirk
Published in
Chembiochem : a European journal of chemical biology
The transpeptidase sortase A of Staphylococcus aureus (Sa-SrtA) is a valuable tool in protein chemistry. The native enzyme anchors surface proteins containing a highly conserved LPxTG sorting motif to a terminal glycine residue of the peptidoglycan layer in gram-positive bacteria. This reaction is exploited for sortase-mediated ligation (SML), allo...
Dal Corso, Alberto
Published in
Chembiochem : a European journal of chemical biology
In recent years, novel classes of small hetero-bifunctional compounds have been developed as tools for the treatment of cancer and other diseases through different approaches, from radio/chemotherapy and imaging to immunotherapy and protein degradation. Within this growing research area, progresses in ligand-screening procedures and hit-to-lead opt...
Yin, Xinjian Li, Jiaxin Chen, Senhua Wu, Yuping She, Zhigang Liu, Lan Wang, Yue Gao, Zhizeng
Published in
Chembiochem : a European journal of chemical biology
O-GlcNAc transferase (OGT) involves in many cellular processes and selective OGT inhibitors are valuable tools to investigate O-GlcNAcylation functions, and could potentially lead to therapeutics. However, high-throughput OGT assays that are suitable for large-scale HTS, and can identify inhibitors targeting both acceptor, donor sites, and alloster...
Chen, Nan Wang, Chu
Published in
Chembiochem : a European journal of chemical biology
Nature utilizes a diverse array of protein post-translational modifications (PTMs) to regulate protein structure, activity, localization, and function. Among them, protein 4'-phosphopantetheinylation derived from Coenzyme A (CoA) is an essential PTM for the biosynthesis of fatty acids, polyketides, and non-ribosomal peptides in prokaryotes and euka...
Hua, Xiao Chu, Guo-Chao Li, Yi-Ming
Published in
Chembiochem : a European journal of chemical biology
Ubiquitin chains with distinct topologies play essential roles in eukaryotic cells. Recently, it was discovered that multiple ubiquitin units can be ligated to more than one lysine residue in the same ubiquitin to form diverse branched ubiquitin chains. Although there is increasing evidence implicating these branched chains in a plethora of biologi...
Kaspar, Felix Neubauer, Peter Kurreck, Anke
Published in
Chembiochem : a European journal of chemical biology
The poor solubility of many nucleosides and nucleobases in aqueous solution demands harsh reaction conditions (base, heat, cosolvent) in nucleoside phosphorylase-catalyzed processes to facilitate substrate loading beyond the low millimolar range. This, in turn, requires enzymes which withstand these conditions. Herein we report that the pyrimidine ...
Jiang, Chenyu Meng, Fanyu Mao, Dongsheng Tang, Yuguo Miao, Peng
Published in
Chembiochem : a European journal of chemical biology
In this study, a tetrahedral DNA nanostructure was first self-assembled; this was then conjugated with gold nanoparticles (AuNPs) and carbon nanodots (CDs). The fabricated nanocomposites allow simultaneous analysis of telomerase activity and miRNA with dual fluorescence channels. By further introducing an iRGD peptide sequence, the nanoconjuates ca...
Telzerow, Aline Paris, Juraj Håkansson, Maria González-Sabín, Javier Ríos-Lombardía, Nicolas Gröger, Harald Morís, Francisco Schürmann, Martin Schwab, Helmut Steiner, Kerstin
...
Published in
Chembiochem : a European journal of chemical biology
Amine transaminases (ATAs) are used to synthesize enantiomerically pure amines, which are building blocks for pharmaceuticals and agrochemicals. R-selective ATAs belong to the fold type IV PLP-dependent enzymes, and different sequence-, structure- and substrate scope-based features have been identified in the past decade. However, our knowledge is ...
Chen, Chun-Chi Min, Jian Zhang, Lilan Yang, Yu Yu, Xuejing Guo, Rey-Ting
Published in
Chembiochem : a European journal of chemical biology
Cytochrome P450s are heme-thiolate enzymes that participate in carbon source assimilation, natural compound biosynthesis and xenobiotic metabolism in all kingdoms of life. P450s can catalyze various reactions by using a wide range of organic compounds, thus exhibiting great potential in biotechnological applications. The catalytic reactions of P450...
