Del Arco, Jon Mills, Alberto Gago, Federico Fernández-Lucas, Jesús
Published in
Chembiochem : a European journal of chemical biology
The use of nucleoside 2'-deoxyribosyltransferases (NDTs) as biocatalysts for the industrial synthesis of nucleoside analogues is often hindered by their strict preference for 2'-deoxyribonucleosides. It is shown herein that a highly versatile purine NDT from Trypanosoma brucei (TbPDT) can also accept ribonucleosides as substrates; this is most like...
Pongpamorn, Pornkanok Watthaisong, Pratchaya Pimviriyakul, Panu Jaruwat, Aritsara Lawan, Narin Chitnumsub, Penchit Chaiyen, Pimchai
Published in
Chembiochem : a European journal of chemical biology
HadA is a flavin-dependent monooxygenase that can catalyze the denitration and dehalogenation of a wide variety of toxicants such as pesticides. Although these enzymatic reactions are useful for bioremediation or biocatalysis, the application of HadA for these purposes is not yet possible because of its low thermostability. In this work we have eng...
Olagnon, Charlotte Monjaras Feria, Julia Grünwald-Gruber, Clemens Blaukopf, Markus Valvano, Miguel A Kosma, Paul
Published in
Chembiochem : a European journal of chemical biology
4-Amino-4-deoxy-l-arabinopyranose (Ara4N) residues have been linked to antibiotic resistance due to reduction of the negative charge in the lipid A and core regions of the bacterial lipopolysaccharide (LPS). To study the enzymatic transfer of Ara4N onto lipid A, which is catalysed by the ArnT transferase, we chemically synthesised a series of anome...
Bartsch, Lina Bartel, Maria Gigante, Alba Iglesias-Fernández, Javier Ruiz-Blanco, Yasser B Beuck, Christine Briels, Jeroen Toetsch, Niklas Bayer, Peter Sanchez-Garcia, Elsa
...
Published in
Chembiochem : a European journal of chemical biology
Modulation of protein-protein interactions (PPIs) is essential for understanding and tuning biologically relevant processes. Although inhibitors for PPIs are widely used, the field still lacks the targeted design of stabilizers. Here, we report unnatural stabilizers based on the combination of multivalency effects and the artificial building block ...
Dohno, Chikara Nakatani, Kazuhiko
Published in
Chembiochem : a European journal of chemical biology
We introduce the concept of molecular glues for RNA, in which specific RNA-binding small molecules induce designed structural changes in target functional RNAs, resulting in modulation of the functions. (Z)-NCTS is an RNA-mismatch-binding small molecule that recognizes 5'-r(XGG)-3'/5'-r(XGG)-3' sequences (X=U or A) and acts as a molecular glue for ...
Huigens, Robert W 3rd Abouelhassan, Yasmeen Yang, Hongfen
Published in
Chembiochem : a European journal of chemical biology
Bacterial biofilms are surface-attached communities of slow-growing and non-replicating persister cells that demonstrate high levels of antibiotic tolerance. Biofilms occur in nearly 80 % of infections and present unique challenges to our current arsenal of antibiotic therapies, all of which were initially discovered for their abilities to target r...
Renfrew, Anna K Karges, Johannes Scopelliti, Rosario Bobbink, Felix D Nowak-Sliwinska, Patrycja Gasser, Gilles Dyson, Paul J
Published in
Chembiochem : a European journal of chemical biology
Cancer is currently one of the deadliest diseases worldwide. Based on the high incidence of this disease, the side effects associated with current chemotherapies and the appearance of drug resistance, considerable efforts have been directed towards the development of new anticancer drugs with new modes of action. Metal-based compounds are particula...
He, Lichun Hiller, Sebastian
Published in
Chembiochem : a European journal of chemical biology
Molecular chaperones are crucial for cellular life to ensure that all proteins obtain their right fold and functionality. Many chaperones promiscuously bind a wide spectrum of client proteins, ranging from nascent to quasi-native and native proteins. Several recent studies have investigated, at atomic resolution, how chaperones interact with native...
Scott, Alan F Luk, Louis Y-P Tuñón, Iñaki Moliner, Vicent Allemann, Rudolf K
Published in
Chembiochem : a European journal of chemical biology
An unsolved mystery in biology concerns the link between enzyme catalysis and protein motions. Comparison between isotopically labelled "heavy" dihydrofolate reductases and their natural-abundance counterparts has suggested that the coupling of protein motions to the chemistry of the catalysed reaction is minimised in the case of hydride transfer. ...
Benedetti, Anna Mary Gill, Daniel M Tsang, Chi W Jones, Alan M
Published in
Chembiochem : a European journal of chemical biology
Sulfation of the amino acid residues of proteins is an emerging post-translational modification, the functions of which are yet to be fully understood. Current sulfation methods are limited mainly to O -tyrosine (sY) which requires negatively charged species around the desired amino acid residue and a specific sulfotransferase enzyme. Alternatively...
