Peng, Tianyuan Nagy, Gabe Trinidad, Jonathan C Jackson, Joy Marie Pohl, Nicola L B
Published in
Chembiochem : a European journal of chemical biology
The most commonly employed glycosidase assays rely on bulky ultraviolet or fluorescent tags at the anomeric position in potential carbohydrate substrates, thereby limiting the utility of these assays for broad substrate characterization. Here we report a qualitative mass spectrometry-based glycosidase assay amenable to high-throughput screening for...
Hirayama, Akane Chu, Jinmiao Goto, Ena Kudo, Fumitaka Eguchi, Tadashi
Published in
Chembiochem : a European journal of chemical biology
The unique five-membered aminocyclitol core of the antitumor antibiotic pactamycin originates from D-glucose, so unprecedented enzymatic modifications of the sugar intermediate are involved in the biosynthesis. However, the order of the modification reactions remains elusive. Here, we examined the timing of introduction of an amino group into certa...
Bechthold, Andreas Zhang, Songya Zhu, Jing Zechel, David Jessen-Trefzer, Claudia T Eastman, Richard Paululat, Thomas
Published in
Chembiochem : a European journal of chemical biology
In this study we report that Streptomyces asterosporus DSM 41452 is a producer of new molecules related to the non-ribosomal cyclodepsipeptide WS9326A and the polyketide Annimycin. S. asterosporus DSM 41452 is shown to produce six cyclodepsipeptides and peptides, WS9326A to G. Notably, the compounds WS9326F and WS9326G have not been de-scribed befo...
Ishikawa, Fumihiro Kasai, Shota Kakeya, Hideaki Tanabe, Genzoh
Published in
Chembiochem : a European journal of chemical biology
Structural and activity studies have revealed the dynamic and transient actions of carrier protein (CP) activity in primary and secondary metabolic pathways. CP-mediated interactions play a central role in nonribosomal peptide biosynthesis, as they serve as covalent tethers for amino acid and aryl acid substrates and enable the growth of peptide in...
Dai, Yumin Kizjakina, Karina Campbell, Ashley C Korasick, David A Tanner, John J Sobrado, Pablo
Published in
Chembiochem : a European journal of chemical biology
The flavin-dependent enzyme 2-haloacrylate hydratase (2-HAH) catalyzes the conversion of 2-chloroacrylate, a major component in the manufacture of acrylic polymers, to pyruvate. The enzyme from has been expressed in Escherichia coli, purified, and characterized. 2-HAH is monomeric in solution and contains a non-covalent, yet tightly bound, FAD. Alt...
Loizzi, Marianna González, Veronica Miller, David J Allemann, Rudolf K
Published in
Chembiochem : a European journal of chemical biology
δ-Cadinene synthase is a sesquiterpene cyclase that utilises the universal achiral precursor farnesyl diphosphate (FDP) to generate predominantly the bicyclic sesquiterpene δ-cadinene and about 2 % germacradien-4-ol, which is also generated from FDP by the cyclase germacradien-4-ol synthase. Herein, the mechanism by which sesquiterpene synthases di...
Lyon, Seth Gopalan, Venkat
Published in
Chembiochem : a European journal of chemical biology
Spectroscopic methods, which are used to establish RNA structure-function relationships, require strategies for post-synthetic, site-specific incorporation of chemical probes into target RNAs. For RNAs >50 nt, the enzymatic incorporation of a nucleoside or nucleotide monophosphate guanosine analog (G-analog) at their 5'-end is routinely achieved by...
Meissner, Murray P Nordblad, Mathias Woodley, John M
Published in
Chembiochem : a European journal of chemical biology
As the application of biocatalysis to complement conventional chemical and catalytic approaches continues to expand, an increasing number of reactions involve poorly-water soluble substrates. At required industrial concentrations necessary for industrial implementation, this frequently leads to heterogeneous reaction mixtures composed of multiple p...
Angelbello, Alicia J Disney, Matthew D
Published in
Chembiochem : a European journal of chemical biology
Noncoding RNAs are pervasive in cells and contribute to diseases such as cancer. A question in biomedical research is whether noncoding RNAs are targets of medicines. Bleomycin is a natural product that cleaves DNA; however, it is known to cleave RNA in vitro. Herein, an in-depth analysis of the RNA cleavage preferences of bleomycin A5 is presented...
Horwath, Michael C Bell-Horwath, Tiffany R Lescano, Victor Krishnan, Karthik Merino, Edward J Deepe, George S Jr
Published in
Chembiochem : a European journal of chemical biology
Ferrostatin-1 (Fer-1) is a lipophilic antioxidant that effectively blocks ferroptosis, a distinct non-apoptotic form of cell death caused by lipid peroxidation. During many infections, both pathogens and host cells are subjected to oxidative stress, but the occurrence of ferroptosis had not been investigated. We examined ferroptosis in macrophages ...
