Mirzakhalili, Ehsan Barra, Beatrice Capogrosso, Marco Lempka, Scott F
Published in
Cell systems
Temporal interference (TI) is a non-invasive neurostimulation technique that utilizes high-frequency external electric fields to stimulate deep neuronal structures without affecting superficial, off-target structures. TI represents a potential breakthrough for treating conditions, such as Parkinson's disease and chronic pain. However, early clinica...
Hark, Timothy J. Rao, Nalini R. Castillon, Charlotte Basta, Tamara Smukowski, Samuel Bao, Huan Upadhyay, Arun Bomba-Warczak, Ewa Nomura, Toshihiro O’Toole, Eileen T.
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Published in
Cell systems
Compromised protein homeostasis underlies accumulation of plaques and tangles in Alzheimer’s disease (AD); however, little is known about the early mechanisms that contribute to this process. To objectively assess protein turnover at early stages of amyloid beta (Aβ) proteotoxicity, we used dynamic 15N metabolic labeling followed by proteomic analy...
Shim, Woo Jun Sinniah, Enakshi Xu, Jun Vitrinel, Burcu Alexanian, Michael Andreoletti, Gaia Shen, Sophie Sun, Yuliangzi Balderson, Brad Boix, Carles
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Published in
Cell systems
Velazquez, Jeremy J LeGraw, Ryan Moghadam, Farzaneh Tan, Yuqi Kilbourne, Jacquelyn Maggiore, Joseph C Hislop, Joshua Liu, Silvia Cats, Davy Chuva de Sousa Lopes, Susana M
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Published in
Cell systems
Pluripotent stem cell (PSC)-derived organoids have emerged as novel multicellular models of human tissue development but display immature phenotypes, aberrant tissue fates, and a limited subset of cells. Here, we demonstrate that integrated analysis and engineering of gene regulatory networks (GRNs) in PSC-derived multilineage human liver organoids...
Hawkins, John S Silvis, Melanie R Koo, Byoung-Mo Peters, Jason M Osadnik, Hendrik Jost, Marco Hearne, Cameron C Weissman, Jonathan S Todor, Horia Gross, Carol A
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Published in
Cell systems
Essential genes are the hubs of cellular networks, but lack of high-throughput methods for titrating gene expression has limited our understanding of the fitness landscapes against which their expression levels are optimized. We developed a modified CRISPRi system leveraging the predictable reduction in efficacy of imperfectly matched sgRNAs to gen...
Stewman, Shannon F Tsui, Kenneth K Ma, Ao
Published in
Cell systems
Microtubules are the backbone of the cytoskeleton and vital to numerous cellular processes. The central dogma of microtubules is that all their functions are driven by dynamic instability, but its mechanism has remained unresolved for over 30 years because of conceptual difficulties inherent in the dominant GTP-cap framework. We present a physicall...
Chen, Yvonne Y. Lim, Wendell Levings, Megan Blazar, Bruce Krenciute, Giedre Wong, Wilson Lehner, Manfred
Published in
Cell systems
Batut, Bérénice Hiltemann, Saskia Bagnacani, Andrea Baker, Dannon Bhardwaj, Vivek Blank, Clemens Bretaudeau, Anthony Brillet-Guéguen, Loraine Čech, Martin Chilton, John
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Published in
Cell systems
The primary problem with the explosion of biomedical datasets is not the data, not computational resources, and not the required storage space, but the general lack of trained and skilled researchers to manipulate and analyze these data. Eliminating this problem requires development of comprehensive educational resources. Here we present a communit...
Chen, Susan Y Osimiri, Lindsey C Chevalier, Michael Bugaj, Lukasz J Nguyen, Taylor H Greenstein, R A Ng, Andrew H Stewart-Ornstein, Jacob Neves, Lauren T El-Samad, Hana
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Published in
Cell systems
Gene expression is thought to be affected not only by the concentration of transcription factors (TFs) but also the dynamics of their nuclear translocation. Testing this hypothesis requires direct control of TF dynamics. Here, we engineer CLASP, an optogenetic tool for rapid and tunable translocation of a TF of interest. Using CLASP fused to Crz1, ...
Baker, Gregory J Muhlich, Jeremy L Palaniappan, Sucheendra K Moore, Jodene K Davis, Stephanie H Santagata, Sandro Sorger, Peter K
Published in
Cell systems
Accurately profiling systemic immune responses to cancer initiation and progression is necessary for understanding tumor surveillance and, ultimately, improving therapy. Here, we describe the SYLARAS software tool (systemic lymphoid architecture response assessment) and a dataset collected with SYLARAS that describes the frequencies of immune cells...