Guo, Qirui Zhao, Yingchi Li, Junhong Liu, Jiangning Yang, Xiuhong Guo, Xuefei Kuang, Ming Xia, Huawei Zhang, Zeming Cao, Lili
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Published in
Cell host & microbe
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic poses an unprecedented public health crisis. Evidence suggests that SARS-CoV-2 infection causes dysregulation of the immune system. However, the unique signature of early immune responses remains elusive. We characterized the transcriptome of rhesus macaques and mice infected...
Hoffmann, H-Heinrich Sánchez-Rivera, Francisco J Schneider, William M Luna, Joseph M Soto-Feliciano, Yadira M Ashbrook, Alison W Le Pen, Jérémie Leal, Andrew A Ricardo-Lax, Inna Michailidis, Eleftherios
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Published in
Cell host & microbe
The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has devastated the global economy and claimed more than 1.7 million lives, presenting an urgent global health crisis. To identify host factors required for infection by SARS-CoV-2 and seasonal coronaviruses, we designed a focused high-coverage CRISPR-Cas9 library targ...
Ku, Min-Wen Bourgine, Maryline Authié, Pierre Lopez, Jodie Nemirov, Kirill Moncoq, Fanny Noirat, Amandine Vesin, Benjamin Nevo, Fabien Blanc, Catherine
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Published in
Cell host & microbe
To develop a vaccine candidate against coronavirus disease 2019 (COVID-19), we generated a lentiviral vector (LV) eliciting neutralizing antibodies against the Spike glycoprotein of SARS-CoV-2. Systemic vaccination by this vector in mice, in which the expression of the SARS-CoV-2 receptor hACE2 has been induced by transduction of respiratory tract ...
Greaney, Allison J Starr, Tyler N Gilchuk, Pavlo Zost, Seth J Binshtein, Elad Loes, Andrea N Hilton, Sarah K Huddleston, John Eguia, Rachel Crawford, Katharine H D
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Published in
Cell host & microbe
Antibodies targeting the SARS-CoV-2 spike receptor-binding domain (RBD) are being developed as therapeutics and are a major contributor to neutralizing antibody responses elicited by infection. Here, we describe a deep mutational scanning method to map how all amino-acid mutations in the RBD affect antibody binding and apply this method to 10 human...
Lu, Maolin Uchil, Pradeep D Li, Wenwei Zheng, Desheng Terry, Daniel S Gorman, Jason Shi, Wei Zhang, Baoshan Zhou, Tongqing Ding, Shilei
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Published in
Cell host & microbe
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) mediates viral entry into cells and is critical for vaccine development against coronavirus disease 2019 (COVID-19). Structural studies have revealed distinct conformations of S, but real-time information that connects these structures is lacking. Here we apply single-molecu...
Cortese, Mirko Lee, Ji-Young Cerikan, Berati Neufeldt, Christopher J Oorschot, Viola M J Köhrer, Sebastian Hennies, Julian Schieber, Nicole L Ronchi, Paolo Mizzon, Giulia
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Published in
Cell host & microbe
Pathogenesis induced by SARS-CoV-2 is thought to result from both an inflammation-dominated cytokine response and virus-induced cell perturbation causing cell death. Here, we employ an integrative imaging analysis to determine morphological organelle alterations induced in SARS-CoV-2-infected human lung epithelial cells. We report 3D electron micro...
Durrant, Matthew G Bhatt, Ami S
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Cell host & microbe
Small open reading frames (smORFs) and their encoded microproteins play central roles in microbes. However, there is a vast unexplored space of smORFs within human-associated microbes. A recent bioinformatic analysis used evolutionary conservation signals to enhance prediction of small protein families. To facilitate the annotation of specific smOR...
Weissman, Drew Alameh, Mohamad-Gabriel de Silva, Thushan Collini, Paul Hornsby, Hailey Brown, Rebecca LaBranche, Celia C Edwards, Robert J Sutherland, Laura Santra, Sampa
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Published in
Cell host & microbe
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein acquired a D614G mutation early in the pandemic that confers greater infectivity and is now the globally dominant form. To determine whether D614G might also mediate neutralization escape that could compromise vaccine efficacy, sera from spike-immunized mice, nonhuman pr...
Hewitt, Judith A Lutz, Cathleen Florence, William C Pitt, M Louise M Rao, Srinivas Rappaport, Jay Haigwood, Nancy L
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Cell host & microbe
The Preclinical Working Group of Accelerating COVID-19 Therapeutic Interventions and Vaccines (ACTIV), a public-private partnership spearheaded by the National Institutes of Health, has been charged with identifying, prioritizing, and communicating SARS-CoV-2 preclinical resources. Reviewing SARS-CoV-2 animal model data facilitates standardization ...
Grobler, Jay A Anderson, Annaliesa S Fernandes, Prabhavathi Diamond, Michael S Colvis, Christine M Menetski, Joseph P Alvarez, Rosa M Young, John A T Carter, Kara L
Published in
Cell host & microbe
When SARS-CoV-2 emerged at the end of 2019, no approved therapeutics or vaccines were available. An urgent need for countermeasures during this crisis challenges the current paradigm of traditional drug discovery and development, which usually takes years from start to finish. Approaches that accelerate this process need to be considered. Here we p...