Bayerl, Jonathan Ayyash, Muneef Shani, Tom Manor, Yair Shlomo Gafni, Ohad Massarwa, Rada Kalma, Yael Aguilera-Castrejon, Alejandro Zerbib, Mirie Amir, Hadar
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Published in
Cell Stem Cell
Engineered systems were used to screen for conditions that enable robust induction of human naive PSCs without the obligation for exogenous transgenes or feeder cells. The latter allowed defining the signaling and transcriptional foundations of human naive PSCs with enhanced (epi)genetic stability and competence for differentiation into all lineage...
Mertens, Jerome Herdy, Joseph R Traxler, Larissa Schafer, Simon T Schlachetzki, Johannes C M Böhnke, Lena Reid, Dylan A Lee, Hyungjun Zangwill, Dina Fernandes, Diana P
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Published in
Cell stem cell
Sporadic Alzheimer's disease (AD) exclusively affects elderly people. Using direct conversion of AD patient fibroblasts into induced neurons (iNs), we generated an age-equivalent neuronal model. AD patient-derived iNs exhibit strong neuronal transcriptome signatures characterized by downregulation of mature neuronal properties and upregulation of i...
Nakamura, Yoshiaki Jörg, David J Kon, Yayoi Simons, Benjamin D Yoshida, Shosei
Published in
Cell stem cell
A remarkable feature of tissue stem cells is their ability to regenerate the structure and function of host tissue following transplantation. However, the dynamics of donor stem cells during regeneration remains largely unknown. Here we conducted quantitative clonal fate studies of transplanted mouse spermatogonial stem cells in host seminiferous t...
Dray, Nicolas Mancini, Laure Binshtok, Udi Cheysson, Felix Supatto, Willy Mahou, Pierre Bedu, Sébastien Ortica, Sara Than-Trong, Emmanuel Krecsmarik, Monika
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Published in
Cell stem cell
Neural stem cell (NSC) populations persist in the adult vertebrate brain over a lifetime, and their homeostasis is controlled at the population level through unknown mechanisms. Here, we combine dynamic imaging of entire NSC populations in their in vivo niche over several weeks with pharmacological manipulations, mathematical modeling, and spatial ...
Grossmann, Sebastian Hooks, Yvette Wilson, Laura Moore, Luiza O'Neill, Laura Martincorena, Iñigo Voet, Thierry Stratton, Michael R Heer, Rakesh Campbell, Peter J
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Published in
Cell stem cell
Clonal dynamics and mutation burden in healthy human prostate epithelium are relevant to prostate cancer. We sequenced whole genomes from 409 microdissections of normal prostate epithelium across 8 donors, using phylogenetic reconstruction with spatial mapping in a 59-year-old man's prostate to reconstruct tissue dynamics across the lifespan. Somat...
Yanagida, Ayaka Spindlow, Daniel Nichols, Jennifer Dattani, Anish Smith, Austin Guo, Ge
Published in
Cell Stem Cell
Reliable stem cell models of the early embryo would open new approaches to studying human development and infertility. Yanagida and colleagues used naive stem cells to generate human blastocyst-like structures comprising the three founding tissue layers for the extraembryonic membranes and the embryo.
Guo, Ge Stirparo, Giuliano Giuseppe Strawbridge, Stanley E. Spindlow, Daniel Yang, Jian Clarke, James Dattani, Anish Yanagida, Ayaka Li, Meng Amy Myers, Sam
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Published in
Cell Stem Cell
Human pluripotent stem cells (hPSCs) exist in naive or primed states, but it has been unclear whether these correspond to distinct developmental potencies. Here, Guo et al. show that naive hPSCs differentiate into trophectoderm, the founder tissue of the placenta, whereas primed hPSCs have lost this potential and instead form the amnion.
Huang, Ling Desai, Ridhdhi Conrad, Daniel N Leite, Nayara C Akshinthala, Dipikaa Lim, Christine Maria Gonzalez, Raul Muthuswamy, Lakshmi B Gartner, Zev Muthuswamy, Senthil K
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Published in
Cell stem cell
The exocrine pancreas, consisting of ducts and acini, is the site of origin of pancreatitis and pancreatic ductal adenocarcinoma (PDAC). Our understanding of the genesis and progression of human pancreatic diseases, including PDAC, is limited because of challenges in maintaining human acinar and ductal cells in culture. Here we report induction of ...
Eriksen, Anne Z. Møller, Rasmus Makovoz, Bar Uhl, Skyler A. tenOever, Benjamin R. Blenkinsop, Timothy A.
Published in
Cell Stem Cell
Eriksen et al. show that SARS-CoV-2 antigens are expressed in COVID-19 patient samples and find robust infection of cadaveric ocular surface cells. Limbus cells derived from human donors and a hPSC whole-eye differentiation model seem more prone to infection and exhibit robust chemokine production and an impaired IFN-I/-III response.
Harris, Lachlan Rigo, Piero Stiehl, Thomas Gaber, Zachary B. Austin, Sophie H.L. Masdeu, Maria del Mar Edwards, Amelia Urbán, Noelia Marciniak-Czochra, Anna Guillemot, François
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Published in
Cell Stem Cell
Harris et al. show that multiple cellular changes work in concert during early life to preserve the hippocampal stem cell population throughout adulthood in mice. In particular, more proliferating stem cells return to quiescence instead of differentiating. The changes are coordinated by increasing degradation of the pro-activation factor ASCL1.
