COVID-19 and the human innate immune system
Published in Cell
Differences in innate immune system function may underpin the curiously variable disease spectrum of COVID-19.
Human neutralizing antibodies against SARS-CoV-2 require intact Fc effector functions for optimal therapeutic protection...
...Published in Cell
Neutralizing human monoclonal antibodies (mAbs) against SARS-CoV-2 require Fc effector functions for optimal protection during post-exposure therapy, with intact mAbs reducing SARS-CoV-2 burden and lung disease in rodent models better than LALA-PG loss-of-function Fc variant mAbs and requiring monocytes and CD8+ T cells for optimal clinical and vir...
Time-resolved Systems Immunology Reveals a Late Juncture Linked to Fatal COVID-19
...Published in Cell
A multiparameter metric developed by integrating circulating cytokines, multimodal single cell profiling, cell surface protein and immune cell analyses of COVID-19 patients over time, reveals a late wave of diverging inflammatory and host immune responses that predicts recovery versus fatality.
In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs...
...Published in Cell
Sun et al. determined the SARS-CoV-2 RNA genome structure in infected cells and from refolded RNAs, which enabled prediction of 42 host proteins that bind to viral RNA using a deep learning tool, and identification of FDA-approved drugs for repurposing to reduce SARS-CoV-2 infection in cells.
Seasonal human coronavirus antibodies are boosted upon SARS-CoV-2 infection but not associated with protection
...Published in Cell
Analysis of human serum samples before and after the onset of the COVID-19 pandemic show that antibodies against common seasonal human coronaviruses are cross-reactive against SARS-CoV-2 but do not confer cross-protection against infection or hospitalization.
Reconstruction of motor control circuits in adult Drosophila using automated transmission electron microscopy.
...Published in Cell
To investigate circuit mechanisms underlying locomotor behavior, we used serial-section electron microscopy (EM) to acquire a synapse-resolution dataset containing the ventral nerve cord (VNC) of an adult female Drosophila melanogaster. To generate this dataset, we developed GridTape, a technology that combines automated serial-section collection w...
Spatiotemporal analysis of human intestinal development at single-cell resolution.
...Published in Cell
Development of the human intestine is not well understood. Here, we link single-cell RNA sequencing and spatial transcriptomics to characterize intestinal morphogenesis through time. We identify 101 cell states including epithelial and mesenchymal progenitor populations and programs linked to key morphogenetic milestones. We describe principles of ...
COVID-19 immune features revealed by a large-scale single cell transcriptome atlas
...Published in Cell
Analysis of immune landscape in the lung and peripheral blood of COVID patients across different regions in China at the single cell level documents the presence of viral RNAs in diverse cell types and highlights the potential contribution of megakaryocytes and monocyte subsets to cytokine storms.
Public health actions to control new SARS-CoV-2 variants
Published in Cell
Recent reports suggest that some SARS-CoV-2 genetic variants, such as B.1.1.7, might be more transmissible and are quickly spreading around the world. As the emergence of more transmissible variants could exacerbate the pandemic, we provide public health guidance for increased surveillance and measures to reduce community transmission.
Circulating SARS-CoV-2 spike N439K variants maintain fitness while evading antibody-mediated immunity
...Published in Cell
Epidemiological, clinical, molecular, and structural characterization of the N439K mutation in the SARS-CoV-2 spike receptor binding motif demonstrates that it results in similar viral fitness compared to wild-type, while conferring resistance against some neutralizing monoclonal antibodies and reducing the activity of some polyclonal antibody resp...
