Yu, Zhao Moomaw, John F Thyagarajapuram, Nagarajan R Geng, Steven B Bent, Colin James Tang, Yu
Published in
Biotechnology progress
Ultrafiltration/diafiltration (UF/DF) is a typical step in protein drug manufacturing process to concentrate and exchange the protein solution into a desired formulation. However, significant offset of pH and composition from the target formulation have been frequently observed after UF/DF, posing challenges to the stability, performance, and consi...
Arias, Cesar Andres Diaz Matsudo, Marcelo Chuei Ferreira-Camargo, Livia Seno Molino, João Vitor Dutra Mayfield, Stephen Patrick de Carvalho, João Carlos Monteiro
Published in
Biotechnology progress
Biotechnology advances have allowed bacteria, yeasts, plants, mammalian and insect cells to function as heterologous protein expression systems. Recently, microalgae have gained attention as an innovative platform for recombinant protein production, due to low culture media cost, compared to traditional systems, as well as the fact that microalgae ...
Torres, Mauro Elvin, Mark Betts, Zeynep Place, Svetlana Gaffney, Claire Dickson, Alan J
Published in
Biotechnology progress
Culture systems based on spin tube reactors have been consolidated in the development of manufacturing processes based on Chinese hamster ovary (CHO) cells. Despite their widespread use, there is little information about the consequences of varying operational setting parameters on the culture performance of recombinant CHO cell lines. Here, we inv...
Kuttiyathil, Mohamed S Mohamed, Mohamed M Al-Zuhair, Sulaiman
Published in
Biotechnology progress
Crude petroleum oil spills are among the most important organic contaminations. While the separated oils accumulated on the surface water are relatively easily removed, the emulsified portions are more difficult to remove and pose significant threats to the environment. Bioremediation using bacteria has proven to be an effective method, but the bio...
Horiguchi, Ikki Torizal, Fuad Gandhi Nagate, Hotaka Inose, Haruka Inamura, Kousuke Hirata, Osamu Hayashi, Hisato Horikawa, Masato Sakai, Yasuyuki
Published in
Biotechnology progress
Suspension culture is an important method used in the industrial preparation of pluripotent stem cells (PSCs), for regenerative therapy and drug screening. Generally, a suspension culture requires agitation to keep PSC aggregates suspended and to promote mass transfer, but agitation also causes cell damage. In this study, we investigated the use of...
Cornet, Jean-François Dussap, Claude-Gilles
Published in
Biotechnology progress
This article establishes and discusses the consistency and the range of applicability of a simple but general and predictive analytical formula, enabling to easily assess the maximum volumetric biomass growth rates (the productivities) in several kinds of photobioreactors with more or less 15% of deviation. Experimental validations are performed on...
Schmitt, Matthew G White, Regina N Barnard, Gavin C
Published in
Biotechnology progress
We developed a simple transient Chinese Hamster Ovary expression platform. Titers for a random panel of 20 clinical monoclonal antibodies (mAbs) ranged from 0.6 to 2.7 g/L after 7 days. Two factors were the key in obtaining these high titers. First, we utilized an extremely high starting cell density (20 million cells/ml), and then arrested further...
Johnstone, Patrick Mast, Elena Hughes, Erik Peng, Haofan
Published in
Biotechnology progress
Perfusion technology has been identified as a process improvement capable of eliminating some of the constraints in cell culture and allows for high cell densities and viabilities. However, when implementing this N-1 seed perfusion platform in large-scale manufacturing, unexpected cell damage was observed as early as Day 1. Given that the shear rat...
Tofani, Larissa B Abriata, Juliana P Luiz, Marcela T Marchetti, Juliana M Swiech, Kamilla
Published in
Biotechnology progress
The acquired drug chemoresistance represents the main challenge of the ovarian cancer treatment. In addition, the absence of an adequate in vitro model able to reproduce the native tumor environment can contribute to the poor success rate of pre-clinical studies of new compounds. Three-dimensional (3D) culture models have been recently used for dru...
Markert, Sven Torkler, Stephanie Hohmann, Katharina Popp, Oliver
Published in
Biotechnology progress
The use of high-throughput systems in cell culture process optimization offers various opportunities in biopharmaceutical process development. Here we describe the potential for acceleration and enhancement of product quality optimization and de novo bioprocess design regarding monoclonal antibody N-glycosylation by using an iterative statistical D...
