Tobin, Tyler Gustafson, Rick Bura, Renata Gough, Heidi L.
Published in
Biotechnology for Biofuels
BackgroundProduction and use of bio-based products offer advantages over conventional petrochemicals, yet the relatively high cost of production has restricted their mainstream adoption. Optimization of wastewater treatment processes could reduce capital expenditures, lowering the barrier to market entry for lignocellulosic biorefineries. This pape...
Swart, Reuben M. le Roux, Francois Naude, Andre de Jongh, Nicolaas W. Nicol, Willie
Published in
Biotechnology for Biofuels
BackgroundThe four-carbon dicarboxylic acids of the tricarboxylic acid cycle (malate, fumarate and succinate) remain promising bio-based alternatives to various precursor chemicals derived from fossil-based feed stocks. The double carbon bond in fumarate, in addition to the two terminal carboxylic groups, opens up an array of downstream reaction po...
Li, Jinyang Zhang, Yongli Li, Jingen Sun, Tao Tian, Chaoguang
Published in
Biotechnology for Biofuels
BackgroundCellulosic biomass is a promising resource for bioethanol production. However, various sugars in plant biomass hydrolysates including cellodextrins, cellobiose, glucose, xylose, and arabinose, are poorly fermented by microbes. The commonly used ethanol-producing microbe Saccharomyces cerevisiae can usually only utilize glucose, although m...
Kim, Eun Jae Kim, Sanghee Choi, Han-Gu Han, Se Jong
Published in
Biotechnology for Biofuels
BackgroundBiofuels, generated using microalgae as sustainable energy, have received a lot of attention. Microalgae can be cultivated at low cost with CO2 and solar energy without competition from edible crops. Psychrophilic microalgae can be a suitable feedstock to produce biofuels without the environmental constraints of low temperatures, because ...
Zhao, Zhe Xian, Mo Liu, Min Zhao, Guang
Published in
Biotechnology for Biofuels
Xylose is a major component of lignocellulose and the second most abundant sugar present in nature. Efficient utilization of xylose is required for the development of economically viable processes to produce biofuels and chemicals from biomass. However, there are still some bottlenecks in the bioconversion of xylose, including the fact that some mi...
Zheng, Wenqiu Lan, Tianqing Li, Hui Yue, Guojun Zhou, Haifeng
Published in
Biotechnology for Biofuels
BackgroundCellulase adsorbed on cellulose is productive and helpful to produce reducing sugars in enzymatic hydrolysis of lignocellulose; however, cellulase adsorbed on lignin is non-productive. Increasing productive adsorption of cellulase on cellulose would be beneficial in improving enzymatic hydrolysis. Adding lignin that was more hydrophilic i...
Ito, Shou Sakai, Kiyota Gamaleev, Vladislav Ito, Masafumi Hori, Masaru Kato, Masashi Shimizu, Motoyuki
Published in
Biotechnology for Biofuels
BackgroundVanillin is the main byproduct of alkaline-pretreated lignocellulosic biomass during the process of fermentable-sugar production and a potent inhibitor of ethanol production by yeast. Yeast cells are usually exposed to vanillin during the industrial production of bioethanol from lignocellulosic biomass. Therefore, vanillin toxicity repres...
Durall, Claudia Lindberg, Pia Yu, Jianping Lindblad, Peter
Published in
Biotechnology for Biofuels
BackgroundCyanobacteria can be metabolically engineered to convert CO2 to fuels and chemicals such as ethylene. A major challenge in such efforts is to optimize carbon fixation and partition towards target molecules.ResultsThe efe gene encoding an ethylene-forming enzyme was introduced into a strain of the cyanobacterium Synechocystis PCC 6803 with...
Gupta, Jai Kumar Rai, Preeti Jain, Kavish Kumar Srivastava, Shireesh
Published in
Biotechnology for Biofuels
BackgroundSynechococcus sp. PCC 7002 is an attractive organism as a feedstock and for photoautotrophic production of biofuels and biochemicals due to its fast growth and ability to grow in marine/brackish medium. Previous studies suggest that the growth of this organism is limited by the HCO3− transport across the cytoplasmic membrane. Tools for ge...
Qiu, Mengyue Shen, Wei Yan, Xiongyin He, Qiaoning Cai, Dongbo Chen, Shouwen Wei, Hui Knoshaug, Eric P. Zhang, Min Himmel, Michael E.
...
Published in
Biotechnology for Biofuels
BackgroundBiofuels and value-added biochemicals derived from renewable biomass via biochemical conversion have attracted considerable attention to meet global sustainable energy and environmental goals. Isobutanol is a four-carbon alcohol with many advantages that make it attractive as a fossil-fuel alternative. Zymomonas mobilis is a highly effici...
