Sun, Jiayi Zhao, Le Shao, Zengyi Shanks, Jacqueline Peebles, Christie A M
Published in
Biotechnology and bioengineering
The monoterpene indole alkaloids vindoline and catharanthine, which are exclusively synthesized in the medicinal plant Catharanthus roseus, are the two important precursors for the production of pharmaceutically important anti-cancer medicines vinblastine and vincristine. Hairy root culture is an ideal platform for alkaloids production due to its i...
Han, Jong Yun Song, Jae Myeong Seo, Sung Hwa Wang, Chonglong Lee, Seung-Goo Lee, Hongweon Kim, Seon-Won Choi, Eui-Sung
Published in
Biotechnology and bioengineering
Metabolite production through a multistep metabolic pathway can often be increased by efficient substrate channeling created by spatial sequestration of the metabolic reactions. Here, Tya, a structural component in the Ty1 retrotransposon element that forms virus-like particles (VLPs) in Saccharomyces cerevisiae, was used to spatially organize enzy...
Cheng, Zhongyi Peplowski, Lukasz Cui, Wenjing Xia, Yuanyuan Liu, Zhongmei Zhang, Jialei Kobayashi, Michihiko Zhou, Zhemin
Published in
Biotechnology and bioengineering
Optically pure compounds are important in the synthesis of fine chemicals. Using directed evolution of enzymes to obtain biocatalysts that can selectively produce high-value chiral chemicals is often time-, money-, and resource-intensive; traditional semi-rational designs based on structural data and docking experiments are still limited due to the...
Zhang, Ai-Hui Liu, Hao-Lin Huang, Shi-Yang Fu, You-Si Fang, Bai-Shan
Published in
Biotechnology and bioengineering
1,3-propanediol production by Clostridium butyricum is a low productivity process due to the long time seed cultivation and thus hinders its industrial scale production. In the present study, repeated batch fermentation coupled with activated carbon adsorption strategy was first established which conduced not only to saving the time of seed cultiva...
Kelly, Ronan M Kowle, Ronald L Lian, Zhirui Strifler, Beth A Witcher, Derrick R Parekh, Bhavin S Wang, Tongtong Frye, Christopher C
Published in
Biotechnology and bioengineering
Cross-linking of the Fcγ receptors expressed on the surface of hematopoietic cells by IgG immune complexes triggers the activation of key immune effector mechanisms, including antibody-dependent cell mediated cytotoxicity (ADCC). A conserved N-glycan positioned at the N-terminal region of the IgG CH 2 domain is critical in maintaining the quaternar...
Calero, Patricia Jensen, Sheila I Bojanovič, Klara Lennen, Rebecca M Koza, Anna Nielsen, Alex T
Published in
Biotechnology and bioengineering
The soil bacterium Pseudomonas putida KT2440 has gained increasing biotechnological interest due to its ability to tolerate different types of stress. Here, the tolerance of P. putida KT2440 toward eleven toxic chemical compounds was investigated. P. putida was found to be significantly more tolerant toward three of the eleven compounds when compar...
Schmölzer, Katharina Lemmerer, Martin Nidetzky, Bernd
Published in
Biotechnology and bioengineering
Glycosyltransferase cascades are promising tools of biocatalysis for natural product glycosylation, but their suitability for actual production remains to be shown. Here, we demonstrate at a scale of 100 g isolated product the integrated biocatalytic production of nothofagin, the natural 3'-C-β-D-glucoside of the polyphenol phloretin. A parallel re...
Schoborg, Jennifer A Hershewe, Jasmine M Stark, Jessica C Kightlinger, Weston Kath, James E Jaroentomeechai, Thapakorn Natarajan, Aravind DeLisa, Matthew P Jewett, Michael C
Published in
Biotechnology and bioengineering
Protein glycosylation, or the attachment of sugar moieties (glycans) to proteins, is important for protein stability, activity, and immunogenicity. However, understanding the roles and regulations of site-specific glycosylation events remains a significant challenge due to several technological limitations. These limitations include a lack of avail...
Gao, Cong Wang, Shihui Hu, Guipeng Guo, Liang Chen, Xiulai Xu, Peng Liu, Liming
Published in
Biotechnology and bioengineering
The application of rational design in reallocating metabolic flux to overproduce desired chemicals is always restricted by the native regulatory network. Here, we demonstrated that in vitro modular pathway optimization combined with in vivo multiplexed combinatorial engineering enables effective characterization of the bottleneck of a complex biosy...
Parker, Stephanie A Amarikwa, Linus Vehar, Kevin Orozco, Raquel Godfrey, Scott Coffman, Jon Shamlou, Parviz Bardliving, Cameron L
Published in
Biotechnology and bioengineering
Insufficient mixing in laminar flow reactors due to diffusion-dominated flow limits their use in applications where narrow residence time distribution (RTD) is required. The aim of this study was to design and characterize a laminar flow (Re 187.7-375.5) tubular reactor for low pH viral inactivation with enhanced radial mixing via the incorporation...
