Mohiuddin, Ismail S Wei, Sung-Jen Yang, In-Hyoung Martinez, Gloria M Yang, Shengping Cho, Eun Jeong Dalby, Kevin N Kang, Min H
Published in
Biotechnology and bioengineering
Amplification-independent c-MYC overexpression is suggested in multiple cancers. Targeting c-MYC activity has therapeutic potential, but efforts thus far have been mostly unsuccessful. To find a druggable target to modulate c-MYC activity in cancer, we identified two kinases, MAPKAPK2 (MK2) and the DNA-dependent protein kinase catalytic subunit (DN...
Anbazhagan, Rajeshkumar Muthusamy, Ganesan Krishnamoorthi, Rajakumari Kumaresan, Swedha Nagarajan, Rajendra Prasad Lai, Juin-Yih Yang, Jen Ming Tsai, Hsieh-Chih
Published in
Biotechnology and bioengineering
In this study, we prepared ferulic acid (FA) and paclitaxel (PTX) co-loaded polyamidoamine (PAMAM) dendrimers conjugated with arginyl-glycyl-aspartic acid (RGD) to overcome P-glycoprotein (P-gp)-mediated multidrug resistance (MDR). FA was released in greater extent (80%) from the outer layer of the dendrimers compared to PTX (70%) from the interior...
Sciuto, Emanuele Luigi Petralia, Salvatore van der Meer, Jan Roelof Conoci, Sabrina
Published in
Biotechnology and bioengineering
The heavy metals pollution represents one of the important issues in the environmental field since it is involved in many pathologies from cancer, neurodegenerative and metabolic diseases. We propose an innovative portable biosensor for the determination of traces of trivalent Arsenic (As(III)) and bivalent mercury (Hg(II)) in water. The system imp...
Lampe, Anna T Farris, Eric J Brown, Deborah M Pannier, Angela K
Published in
Biotechnology and bioengineering
The investigation of new adjuvants is essential for development of efficacious vaccines. Chitosan (CS), a derivative of chitin, has been shown to act as an adjuvant, improving vaccine-induced immune responses. However, the effect of CS molecular weight (MW) on this adjuvanticity has not been investigated, despite MW having been shown to impact CS b...
Farzaneh, Zahra Abbasalizadeh, Saeed Asghari-Vostikolaee, Mohammad-Hassan Alikhani, Mehdi Cabral, Joaquim M S Baharvand, Hossein
Published in
Biotechnology and bioengineering
Developing technologies for scalable production of human organoids has gained increased attention for "organoid medicine" and drug discovery. We developed a scalable and integrated differentiation process for generation of hepatic organoid from human pluripotent stem cells (hPSCs) in a fully controlled stirred tank bioreactor with 150 ml working vo...
Lee, Ki Baek Dunn, Zachary S Lopez, Tyler Mustafa, Zahid Ge, Xin
Published in
Biotechnology and bioengineering
Matrix metalloproteinase-12 (MMP-12), also known as macrophage elastase, is a potent inflammatory mediator and therefore an important pharmacological target. Clinical trial failures of broad-spectrum compound MMP inhibitors suggested that specificity is the key for a successful therapy. To provide the required selectivity, monoclonal antibody (mAb)...
Cortés-Martínez, Carlos Inocencio Chavarría-Hernández, Norberto
Published in
Biotechnology and bioengineering
Monoxenic liquid culture is the most suitable technology for scaling up to industrial production of entomopathogenic nematodes (EPNs); however, the variability of the yield production remains a current problem in the process. The aim of this study was to analyze the parameters and criteria for EPN production in liquid culture based on scientific an...
Khatiwada, Bishal Sunna, Anwar Nevalainen, Helena
Published in
Biotechnology and bioengineering
Euglena gracilis is a promising source of commercially important metabolites such as vitamins, wax esters, paramylon, and amino acids. However, the molecular tools available to create improved Euglena strains are limited compared to other microorganisms that are currently exploited in the biotechnology industry. The complex poly-endosymbiotic natur...
Chemmalil, Letha Prabhakar, Tanushree Kuang, June West, Jay Tan, Zhijun Ehamparanathan, Vivekh Song, Yuanli Xu, Jianlin Ding, Julia Li, Zhengjian
...
Published in
Biotechnology and bioengineering
Process analytical technology (PAT) has been defined by the Food and Drug Administration as a system for designing, analyzing, and controlling manufacturing through timely measurements to ensure final product quality. Based on quality-by-design (QbD) principles, real-time or near-real-time data monitoring is essential for timely control of critical...
Zhang, Liang Ren, Shichao Liu, Xiaofei Liu, Xiaochen Guo, Fang Sun, Wentao Feng, Xudong Li, Chun
Published in
Biotechnology and bioengineering
Pentacyclic triterpenoids have wide applications in the pharmaceutical industry. The precise glucosylation at C-3 OH of pentacyclic triterpenoids mediated by uridine 5'-diphospho-glucosyltransferase (UDP-glucosyltransferase [UGT]) is an important way to produce valuable derivatives with various improved functions. However, most reported UGTs suffer...
