Rosen, Osnat Jayson, Avital Goldvaser, Michael Dor, Eyal Monash, Arik Levin, Lilach Cherry, Lilach Lupu, Edith Natan, Niva Girshengorn, Meni
...
Published in
Biotechnology and bioengineering
To face the coronavirus disease 2019 pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, our institute has developed the rVSV-ΔG-spike vaccine, in which the glycoprotein of vesicular stomatitis virus (VSV) was replaced by the spike protein of SARS-CoV-2. Many process parameters can influence production yield. ...
Wan, Xinyu Volcke, Eveline I P
Published in
Biotechnology and bioengineering
Partial nitration-anammox is a resource-efficient pathway for nitrogen removal from wastewater. However, the advantages of this nitrogen removal technology may be counter-acted by the emission of N2 O, a potent greenhouse gas. In this study, mathematical modelling was applied to analyse N2 O formation and emission dynamics and to develop N2 O mitig...
Barten, Robin Chin-On, Rocca de Vree, Jeroen van Beersum, Ellen Wijffels, Rene H Barbosa, Maria J Janssen, Marcel
Published in
Biotechnology and bioengineering
Multiple models have been developed in the field to simulate growth and product accumulation of microalgal cultures. These models heavily depend on the accurate estimation of growth parameters. In this paper growth parameters are presented for three industrially relevant microalgae species: Nannochloropsis sp., Neochloris oleoabundans, and Picochlo...
Hillebrandt, Nils Vormittag, Philipp Dietrich, Annabelle Hubbuch, Jürgen
Published in
Biotechnology and bioengineering
Virus-like particles (VLPs) are an emerging biopharmaceutical modality with great potential as a platform technology. VLPs can be applied as gene therapy vectors and prophylactic or therapeutic vaccines. For non-enveloped VLPs, recombinant production of the protein subunits leads to intracellular self-assembly. The subsequent purification process i...
Jin, Ci Li, Jing Huang, Zhen Han, Xushen Bao, Jie
Published in
Biotechnology and bioengineering
Lignocellulose is the only feasible carbohydrates feedstock for commercial scale and carbon neutral production of poly(3-hydroxybutyrate) (PHB) biopolymer by its great abundance and availability. Microbial cell factories for fermentative PHB synthesis are highly restricted by the growth suppression of inhibitors from lignocellulose pretreatment. Th...
Edwards, Elizabeth Livanos, Maria Krueger, Anja Dell, Anne Haslam, Stuart M Mark Smales, C Bracewell, Daniel G
Published in
Biotechnology and bioengineering
Glycosylation can be a critical quality attribute in biologic manufacturing. In particular, it has implications on the half-life, immunogenicity, and pharmacokinetics of therapeutic monoclonal antibodies (mAbs), and must be closely monitored throughout drug development and manufacturing. To address this, advances have been made primarily in upstrea...
Dash, Shantoshini Sharon, David M Mullick, Alaka Kamen, Amine A
Published in
Biotechnology and bioengineering
Plasmid transfection of mammalian cells is the dominant platform used to produce adeno-associated virus (AAV) vectors for clinical and research applications. Low yields from this platform currently make it difficult to supply these activities with adequate material. In an effort to better understand the current limitations of transfection-based man...
Acevedo Alonso, Vanessa Lackner, Susanne
Published in
Biotechnology and bioengineering
The behavior of heterotrophic bacteria growing in systems with low or no external supply of chemical oxygen demand (COD) has become more relevant within the wastewater context. Growth strategies help to clarify how bacteria behave and adapt to different environmental conditions. In the case of substrate limited conditions, research has been mainly ...
Mok, Jin Hong Niu, Ye Yousef, Ahmed Zhao, Yi Sastry, Sudhir K
Published in
Biotechnology and bioengineering
Escherichia coli O157:H7 have previously been associated with disease outbreaks associated with leafy green vegetables. However, the physical mechanisms that determine the spatial organization of bacteria onto leafy greens are still not clear. Microfluidics with embedded trichome-mimicking microposts were employed to investigate the role of shear f...
Li, Zonglin Sun, Chuanqi Lou, Longwei Li, Zhimin
Published in
Biotechnology and bioengineering
The pyrimidine metabolic pathway is tightly regulated in microorganisms, allowing limited success in metabolic engineering for the production of pathway-related substances. Here, we constructed a four-enzyme coupled system for the in vitro production of uridine triphosphate (UTP). The enzymes used include nucleoside kinase, uridylate kinase, nucleo...
