Wang, Huanyan Liu, Wanhong Qiu, Fei Chen, Yupei Zhang, Fangyuan Lan, Xiaozhong Chen, Min Zhang, Haoxing Liao, Zhihua
Published in
Biotechnology and applied biochemistry
In recent years, although several related genes had been cloned and characterized, the role of aldehyde dehydrogenase 1 (ALDH1), the newly cloned gene involved in artemisinin biosynthesis pathway, is still not clear. In this study, a 2,100-bp ALDH1 promoter region fused with GUS reporter gene was stably transferred into Arabidopsis thaliana. Histoc...
Liang, Xianjun Liu, Tongjun Zhang, Weizhong Zhang, Kai Guo, Shuai Liang, Jianhua
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Biotechnology and applied biochemistry
M-phase phosphoprotein 8 (MPP8) has been reported to be overexpressed in various human carcinoma cells and was associated with tumor malignant characters. However, its functional role in colon cancer (CRC) is still unclear. In the present study, lentivirus-mediated short hairpin RNAs were designed to silence the MPP8 gene in CRC cells including RKO...
Hsu, Chih-Jung Jain, Harsh V Williams, Abasha Wang, Julie Lute, Scott C Beaucage, Serge L Brorson, Kurt A
Published in
Biotechnology and applied biochemistry
A rapid and cost-effective transient transfection method for mammalian cells is essential for screening biopharmaceuticals in early stages of development. A library of 25 amphipathic trans-acting oligodeoxythymidine phosphorothioate triester (dTtaPS) transfection reagents, carrying positively charged and lipophilic groups, has been constructed for ...
Alhadrami, Hani A
Published in
Biotechnology and applied biochemistry
Biosensors are devices that combine a biological material with a suitable platform for detection of pathogenic organisms, carcinogenic, mutagenic, and/or toxic chemicals or for reporting a biological effect. In recent years, an enormous number of different types of biosensors have been constructed and developed for several medical applications. The...
Wu, Wei-Bin Guo, Xiao-Lei Zhang, Ming-Liang Huang, Qing-Gen Qi, Feng Huang, Jian-Zhong
Published in
Biotechnology and applied biochemistry
l-Phenylalanine is an important amino acid that is widely used in the production of food flavors and pharmaceuticals. Generally, l-phenylalanine production by engineered Escherichia coli requires a high rate of oxygen supply. However, the coexpression of Vitreoscilla hemoglobin gene (vgb), driven bya tac promoter, with the genes encoding 3-deoxy-d-...
Khan, Imran Ansari, Irfan A Singh, Pratichi Dass J, Febin Prabhu
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Biotechnology and applied biochemistry
The phosphatase and tensin homolog (PTEN) gene plays a crucial role in signal transduction by negatively regulating the PI3K signaling pathway. It is the most frequent mutated gene in many human-related cancers. Considering its critical role, a functional analysis of missense mutations of PTEN gene was undertaken in this study. Thirty five nonsynon...
Chen, Jia-Wen Liu, Wan-Jun Hu, Dong-Xiong Wang, Xiang Balamurugan, Srinivasan Alimujiang, Adili Yang, Wei-Dong Liu, Jie-Sheng Li, Hong-Ye
Published in
Biotechnology and applied biochemistry
Oleaginous microalgae hold great promises for biofuel production. However, commercialization of microalgal biofuels remains impracticable due to the lack of suitable industrial strains with high growth rate and lipid productivity. Engineering of metabolic pathways is a potential strategy for the improvement of microalgal strains for the production ...
Tam, Yew Joon Zeenathul, Nazariah Allaudin Rezaei, Morvarid Akhavan Mustafa, Nor Hidayah Azmi, Mohd Lila Mohd Bahaman, Abdul Rani Lo, Sewn Cen Tan, Joo Shun Hani, Homayoun Rasedee, Abdullah
...
Published in
Biotechnology and applied biochemistry
Limit of detection (LOD), limit of quantification, and the dynamic range of detection of hepatitis B surface antigen antibody (anti-HBs) using a surface plasmon resonance (SPR) chip-based approach with Pichia pastoris-derived recombinant hepatitis B surface antigen (HBsAg) as recognition element were established through the scouting for optimal con...
Yu, Ping Chen, Xingge Li, Peng
Published in
Biotechnology and applied biochemistry
Fatty acid, isoprenoid, and alcohol pathways have been successfully engineered to produce biofuels. By introducing three genes, atfA, adhE, and pdc, into Escherichia coli to expand fatty acid pathway, up to 1.28 g/L of fatty acid ethyl esters can be achieved. The isoprenoid pathway can be expanded to produce bisabolene with a high titer of 900 mg/L...
Liu, Wenhui Zhao, Wenbin Lai, Jun Shen, Qi Xu, Yingchun Pan, Liqiang Chen, Shuqing
Published in
Biotechnology and applied biochemistry
Human serum albumin (HSA) and interleukin-1 receptor antagonist (IL1Ra) fusion protein is a potential long-acting drug in the treatment of type 2 diabetes. Previously, the expression level of HSA/IL1Ra in Pichia pastoris was successfully improved by increasing the gene copy number and coexpression with chaperone (protein disulfide isomerase) in our...
