Duran, Hatice Esra Beydemir, Şükrü
Published in
Biotechnology and applied biochemistry
Human carbonic anhydrase VII (hCA VII), a cytosolic enzyme, defends against oxidative stress by preventing reactive oxygen species from forming. In our study, first, hCA VII was cloned into Escherichia coli (One Shot Mach1-T1R) strain by using cDNA of the human brain and successfully expressed. The integrity of the plasmid generated by colony PCR w...
Ahmad, Sajjad Sajjad, Muhammad Altayb, Hisham N Sarim Imam, Syed Alshehri, Sultan Ghoneim, Mohammed M Shahid, Saher Usman Mirza, Muhammad Shahid Nadeem, Muhammad Kazmi, Imran
...
Published in
Biotechnology and applied biochemistry
The processive cellulase (CelO) is an important modular enzyme of Clostridium thermocellum. To study the effect of the carbohydrate-binding module (CBM3b) on the catalytic domain of CelO (GH5), four engineered derivatives of CelO were designed by truncation and terminal fusion of CBM3b. These are CBM at the N-terminus, native form (CelO-BC, 62 kDa)...
Nikkhah, Maryam Karami, Sajedeh Khatami, Seyyed Hossein Taheri-Anganeh, Mortaza Savardashtaki, Amir Mahmoodzadeh, Amir Shabaninejad, Zahra Vakili, Omid Mousavi, Pegah Ghanizadeh Gerayeli, Farhad
...
Published in
Biotechnology and applied biochemistry
Testosterone is an anabolic steroid and a major sex hormone in males. It plays vital roles, including developing the testis, penis, and prostate, increasing muscle and bone, and sperm production. In both men and women, testosterone levels should be in normal ranges. Besides, testosterone and its analogs are major global contributors to doping in sp...
El-Sayed, El-Sayed R Zaki, Amira G
Published in
Biotechnology and applied biochemistry
Based on the broad clinical utility of the immunosuppressant mycophenolic acid (MPA), this article aims to intensify the biosynthetic potential of Penicillium roqueforti for more effective hyperproduction of the drug. Several mutants were generated from irradiation mutagenesis and screened. Two strains (GM1013 and GM1093) presented an elevated MPA ...
Saif, Ahmed Al Shahrani, Mesfer Alshehri, Mohammed Abohassan, Mohammad Alshehri, Mohammed A Radhakrishnan, Suresh Rajagopalan, Prasanna
Published in
Biotechnology and applied biochemistry
Benzylidene chromanones are small molecules, structurally similar to active phytochemicals. Herein, we report one novel benzylidene chromanone, TMF 104, for its bio-efficacies. Its computational docking for Vanin-1, antioxidant, free radical scavenging capacities, antimicrobial effects, and anticancer efficacy were analyzed. TMF 104 predicated stro...
Xu, Chengcheng Wu, Yao Tang, Lili Liang, Yan Zhao, Yang
Published in
Biotechnology and applied biochemistry
One of the main pathological features of Parkinson's disease (PD) is the loss of dopaminergic neurons in the substantia nigra compacta (SNc). Cistanoside A (CA) has a strong neuroprotective effect in PD, but the exact mechanism is unclear. In the present study, the MPTP-stimulated mouse model of PD and MPP+ -treated PD model in the MES23.5 neuronal...
Guo, Yu-Xin Yang, Ya-Ru Qin, Yong Guan, Ti-Kun Yang, Qi-Zhi Wang, Yue-Xing Tang, Shi Zhang, Guo-Qing Chen, Qing-Jun
Published in
Biotechnology and applied biochemistry
Recently, composting cultivation method is widely used in oyster mushroom production. In this study, we focused on the effects of composting processes on nutritional qualities and antioxidant activity of Pleurotus floridanus mushroom fruiting bodies. Three treatments of different composting time (2, 4, and 5 days) were performed with an atmospheric...
Sheikh Beig Goharrizi, Mohammad Ali Kazemi Oskuee, Reza Aleyaghoob, Ghazaleh Mohajeri, Taraneh Mohammadinejad, Arash Rezayi, Majid
Published in
Biotechnology and applied biochemistry
25-Hydroxyvitamin D3 as a main circulating metabolite of vitamin D is usually measured in serum to evaluate the vitamin D status of humans. So, developing an accessible, fast response, sensitive, and selective detection method for 25-hydroxyvitamin D3 is highly important. In this study, we designed a sensitive and selective electrochemical sensor b...
Ma, Tengfei Cai, Haowen Zong, Hong Lu, Xinyao Zhuge, Bin
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Biotechnology and applied biochemistry
Pinene is a commercially important monoterpene that can be prepared using engineered bacterial and yeast species; however, high pinene levels can adversely affect the stability and permeability of microbial membranes leading to significantly reduced growth yields. This study reports that the fluidities and permeabilities of cell membranes of Candid...
Liu, Song Tang, Min-Hui Cheng, Jing-Sheng
Published in
Biotechnology and applied biochemistry
This work isolated a strain named Bacillus amyloliquefaciens HM618 from the soil, which can inhibit the growths of Botrytis cinerea, Rhizoctonia solani, and Escherichia coli DH5α. Based on the results of response surface methodology, the surfactin levels of strain HM618 were elevated from 0.724 to 1.876 g/L and 0.995 to 1.888 g/L under the pure cul...