Liang, Xianjun Liu, Tongjun Zhang, Weizhong Zhang, Kai Guo, Shuai Liang, Jianhua
Published in
Biotechnology and applied biochemistry
M-phase phosphoprotein 8 (MPP8) has been reported to be overexpressed in various human carcinoma cells and was associated with tumor malignant characters. However, its functional role in colon cancer (CRC) is still unclear. In the present study, lentivirus-mediated short hairpin RNAs were designed to silence the MPP8 gene in CRC cells including RKO...
Wang, Huanyan Liu, Wanhong Qiu, Fei Chen, Yupei Zhang, Fangyuan Lan, Xiaozhong Chen, Min Zhang, Haoxing Liao, Zhihua
Published in
Biotechnology and applied biochemistry
In recent years, although several related genes had been cloned and characterized, the role of aldehyde dehydrogenase 1 (ALDH1), the newly cloned gene involved in artemisinin biosynthesis pathway, is still not clear. In this study, a 2,100-bp ALDH1 promoter region fused with GUS reporter gene was stably transferred into Arabidopsis thaliana. Histoc...
Alhadrami, Hani A
Published in
Biotechnology and applied biochemistry
Biosensors are devices that combine a biological material with a suitable platform for detection of pathogenic organisms, carcinogenic, mutagenic, and/or toxic chemicals or for reporting a biological effect. In recent years, an enormous number of different types of biosensors have been constructed and developed for several medical applications. The...
Hsu, Chih-Jung Jain, Harsh V Williams, Abasha Wang, Julie Lute, Scott C Beaucage, Serge L Brorson, Kurt A
Published in
Biotechnology and applied biochemistry
A rapid and cost-effective transient transfection method for mammalian cells is essential for screening biopharmaceuticals in early stages of development. A library of 25 amphipathic trans-acting oligodeoxythymidine phosphorothioate triester (dTtaPS) transfection reagents, carrying positively charged and lipophilic groups, has been constructed for ...
Wu, Wei-Bin Guo, Xiao-Lei Zhang, Ming-Liang Huang, Qing-Gen Qi, Feng Huang, Jian-Zhong
Published in
Biotechnology and applied biochemistry
l-Phenylalanine is an important amino acid that is widely used in the production of food flavors and pharmaceuticals. Generally, l-phenylalanine production by engineered Escherichia coli requires a high rate of oxygen supply. However, the coexpression of Vitreoscilla hemoglobin gene (vgb), driven bya tac promoter, with the genes encoding 3-deoxy-d-...
Zhu, Nengwu Shi, Chaohong Shang, Ru Yang, Chong Xu, Zhiguo Wu, Pingxiao
Published in
Biotechnology and applied biochemistry
The ability of Acidithiobacillus ferrooxidans to oxidize ferrous iron has been extensively studied in bioleaching to recover metal resources. Although immobilization of A. ferrooxidans is of great importance to achieve high bioleaching performance in practical application, the reported approaches of immobilization of A. ferrooxidans are still limit...
Zha, Shenfang Yin, Youping Wang, Yu Huang, Yi Li, Yan Wang, Zhongkang
Published in
Biotechnology and applied biochemistry
Cantharidin, a defensive terpene compound synthesized by the meloid beetle (Coleoptera, Meloidae), is an important anticancer agent. However, there has been little study done on how this compound synthesized by the beetle. In this paper, a farnesyl pyrophosphate synthase (FPPS) gene, designated McFPPS, was isolated from Mylabris cichorii by reverse...
Li, D H Yang, Q Zhou, J S Zhang, Z W Miao, M Y Yang, S S Xu, W D
Published in
Biotechnology and applied biochemistry
Metal prostheses of artificial joints undergo wear, producing numerous metal particles and ions, such as Cr3+ . Cr3+ is considered a key factor leading to aseptic loosening. Many studies focus on the effect of Cr3+ on osteoblasts; however, little is known about the effect of Cr3+ on the B-cell maturation antigen (BCMA) in the osteoblasts. In this s...
Chen, Jia-Wen Liu, Wan-Jun Hu, Dong-Xiong Wang, Xiang Balamurugan, Srinivasan Alimujiang, Adili Yang, Wei-Dong Liu, Jie-Sheng Li, Hong-Ye
Published in
Biotechnology and applied biochemistry
Oleaginous microalgae hold great promises for biofuel production. However, commercialization of microalgal biofuels remains impracticable due to the lack of suitable industrial strains with high growth rate and lipid productivity. Engineering of metabolic pathways is a potential strategy for the improvement of microalgal strains for the production ...
Liu, Wenhui Zhao, Wenbin Lai, Jun Shen, Qi Xu, Yingchun Pan, Liqiang Chen, Shuqing
Published in
Biotechnology and applied biochemistry
Human serum albumin (HSA) and interleukin-1 receptor antagonist (IL1Ra) fusion protein is a potential long-acting drug in the treatment of type 2 diabetes. Previously, the expression level of HSA/IL1Ra in Pichia pastoris was successfully improved by increasing the gene copy number and coexpression with chaperone (protein disulfide isomerase) in our...
