Casciaro, Bruno Dutta, Debarun Loffredo, Maria Rosa Marcheggiani, Stefania McDermott, Alison M Willcox, Mark Dp Mangoni, Maria Luisa
Published in
Biopolymers
Contact lens (CL) wear is a risk factor for development of microbial keratitis, a vision threatening infection of the eye. Adverse events associated with colonization of lenses, especially by the multi-drug resistant and biofilm forming bacterium Pseudomonas aeruginosa remain a major safety issue. Therefore, novel strategies and compounds to reduce...
Vilas Boas, Liana Costa Pereira de Lima, Lídia Maria Pinto Migliolo, Ludovico Mendes, Gabriele Dos Santos de Jesus, Maianne Gonçalves Franco, Octávio Luiz Silva, Paula Andréia
Published in
Biopolymers
Viruses are the major cause of disease and mortality worldwide. Nowadays there are treatments based on antivirals or prophylaxis with vaccines. However, the rising number of reports of viral resistance to current antivirals and the emergence of new types of virus has concerned the scientific community. In this scenario, the search for alternative t...
Moore, Anthony F T Gentry, Riley C Koculi, Eda
Published in
Biopolymers
DbpA is a DEAD-box RNA helicase implicated in RNA structural rearrangements in the peptidyl transferase center. DbpA contains an RNA binding domain, responsible for tight binding of DbpA to hairpin 92 of 23S ribosomal RNA, and a RecA-like catalytic core responsible for double-helix unwinding. It is not known if DbpA unwinds only the RNA helices tha...
Kubas, George Rees, William Caguiat, Jonathan Asch, David Fagan, Diana Cortes, Pedro
Published in
Biopolymers
The present research investigates the identification of amino acid sequences that selectively bind to a pentaerythritol tetranitrate (PETN) explosive surrogate. Through the use of a phage display technique and enzyme-linked immunosorbent assays (ELISA), a peptide library was tested against pentaerythritol trinitrate hemisuccinate (PETNH), a surroga...
Tsiolaki, Paraskevi L Louros, Nikolaos N Zompra, Aikaterini A Hamodrakas, Stavros J Iconomidou, Vassiliki A
Published in
Biopolymers
Over the last 20 years, proinsulin C-peptide emerged as an important player in various biological events. Much time and effort has been spent in exploring all functional features of C-peptide and recording its implications in Diabetes mellitus. Only a few studies, though, have addressed C-peptide oligomerization and link this procedure with Diabete...
Reichert, Detlef Gröger, Stefan Hackel, Christiane
Published in
Biopolymers
To gain new insights into the interaction of proteins and disaccharides, we investigated the hydrodynamic radii, RhProt, of lysozyme molecules in solution and in a ternary protein-sugar-water system by PFG-NMR. Our approach is based on the assumption that the anhydrobiotic properties of disaccharides like trehalose are based on aggregation of sugar...
Reichert, Detlef Gröger, Stefan Hackel, Christiane
Published in
Biopolymers
To gain new insights into the interaction of proteins and disaccharides, we investigated the hydrodynamic radii, RhProt, of lysozyme molecules in solution and in a ternary protein-sugar-water system by PFG-NMR. Our approach is based on the assumption that the anhydrobiotic properties of disaccharides like trehalose are based on aggregation of sugar...
Toniolo, Claudio Crisma, Marco Formaggio, Fernando Alemán, Carlos Ramakrishnan, Chandrasekharan Kalmankar, Neha Balaram, Padmanabhan
Published in
Biopolymers
In this study, we performed a detailed literature survey of the ɛ-turn in peptides and proteins. This three-dimensional structural feature is characterized by an eleven-membered pseudo-cycle closed by an intramolecular backbone…backbone H-bond. Interestingly, in this motif the direction of the N-H…O = C H-bond runs opposite to that of the much more...
Grünenfelder, Claudio E Kisunzu, Jessica K Trapp, Nils Kastl, Robert Wennemers, Helma
Published in
Biopolymers
Crystal structures of catalytically active tripeptides of the general type H-dPro-Pro-Xaa and related N-acetylated analogs were compared. The influence of acylation at the N-terminus, the nature of the C-terminal residue, coordinating groups, and intramolecular hydrogen bonds on the conformation of the tripeptides was examined. Regardless of the pr...
Bortolus, Marco Dalzini, Annalisa Maniero, Anna Lisa Panighel, Giacomo Siano, Alvaro Toniolo, Claudio De Zotti, Marta Formaggio, Fernando
Published in
Biopolymers
Trichogin GA IV is a short-length (10-amino acid long), mostly hydrophobic, peptaibiotic with an N-terminal fatty acyl chain and a C-terminal 1,2-amino alcohol. A cardinal role of the terminal moieties in the cytotoxic activity of trichogin has been recently found. Previously, peptide orientation and dynamics of trichogin analogs in the membrane we...
