Ireland, Sam M Sula, Altin Wallace, B A
Published in
Biopolymers
Purified integral membrane proteins require amphipathic molecules to maintain their solubility in aqueous solutions. These complexes, in turn, are used in studies to characterise the protein structures by a variety of biophysical and structural techniques, including spectroscopy, crystallography, and cryo-electron microscopy. Typically the amphilph...
Fusco, Giuliana Sanz-Hernandez, Maximo Ruggeri, Francesco S Vendruscolo, Michele Dobson, Christopher M De Simone, Alfonso
Published in
Biopolymers
The aggregation process of peptides and proteins is of great relevance as it is associated with a wide range of highly debilitating disorders, including Alzheimer's and Parkinson's diseases. The natural product (-)-epigallocatechin-3-gallate (EGCG) can redirect this process away from amyloid fibrils and towards non-toxic oligomers. In this study we...
Perrella, Fulvio Raucci, Umberto Chiariello, Maria Gabriella Chino, Marco Maglio, Ornella Lombardi, Angela Rega, Nadia
Published in
Biopolymers
Fe(III)-Mimochrome VI (MC6) is a recently reported artificial heme-peptide conjugate system with a high peroxidase-like activity. By design, its structure features a five-coordinated Fe(III)-deuteroporphyrin active site, embedded in a compact α-helix-heme-α-helix "sandwich" motif. Up to now, no detailed MC6 structural characterization is available....
Feis, Alessandro Howes, Barry D Milazzo, Lisa Coppola, Daniela Smulevich, Giulietta
Published in
Biopolymers
The ligand binding characteristics of heme-containing proteins are determined by a number of factors, including the nature and conformation of the distal residues and their capability to stabilize the heme-bound ligand via hydrogen-bonding and electrostatic interactions. In this regard, the heme pockets of truncated hemoglobins (TrHbs) constitute a...
Breslauer, Kenneth J
Published in
Biopolymers
Minetti, Conceição A Sun, Jeffrey Y Jacobs, Daniel P Kang, Inkoo Remeta, David P Breslauer, Kenneth J
Published in
Biopolymers
Bistrand lesions embedded within a single helical turn of tridecameric deoxyoligonucleotide duplexes represent a model system for exploring the impact of clustered lesions that occur in vivo and pose a significant challenge to cellular repair machineries. Such investigations are essential for understanding the forces that dictate lesion-induced mut...
Sakai, Shinji Ohi, Hiromi Hotta, Tomoki Kamei, Hidenori Taya, Masahito
Published in
Biopolymers
Bioprinting has a great potential to fabricate three-dimensional (3D) functional tissues and organs. In particular, the technique enables fabrication of 3D constructs containing stem cells while maintaining cell proliferation and differentiation abilities, which is believed to be promising in the fields of tissue engineering and regenerative medici...
Daumke, Oliver Praefcke, Gerrit J K
Published in
Biopolymers
Ravishankar, H Barth, A Andersson, M
Published in
Biopolymers
P-type ATPase proteins maintain cellular homeostasis and uphold critical concentration gradients by ATP-driven ion transport across biological membranes. Characterization of single-cycle dynamics by time-resolved X-ray scattering techniques in solution could resolve structural intermediates not amendable to for example crystallization or cryo-elect...
Betush, Ria J Urban, Jennifer M Nilsson, Bradley L
Published in
Biopolymers
Amphipathic peptides with alternating polar and nonpolar amino acid sequences efficiently self-assemble into functional β-sheet fibrils as long as the nonpolar residues have sufficient hydrophobicity. For example, the Ac-(FKFE)2 -NH2 peptide rapidly self-assembles into β-sheet bilayer nanoribbons, while Ac-(AKAE)2 -NH2 fails to self-assemble under ...
