Applegate, Matthew B. Spink, Samuel S. Roblyer, Darren
Published in
Biomedical Optics Express
Spatial frequency domain imaging (SFDI) is a widefield diffuse optical measurement technique capable of generating 2D maps of sub-surface absorption and scattering in biological tissue. We developed a new hyperspectral SFDI instrument capable of collecting images at wavelengths from the visible to the near infrared. The system utilizes a custom-bui...
De Silva, Tharindu Chew, Emily Y. Hotaling, Nathan Cukras, Catherine A.
Published in
Biomedical Optics Express
This work reports a deep-learning based registration algorithm that aligns multi-modal retinal images collected from longitudinal clinical studies to achieve accuracy and robustness required for analysis of structural changes in large-scale clinical data. Deep-learning networks that mirror the architecture of conventional feature-point-based regist...
Sahu, Sushant P. Liu, Qianglin Prasad, Alisha Hasan, Syed Mohammad Abid Liu, Qun Rodriguez, Maria Ximena Bastidas Mukhopadhyay, Orna Burk, David Francis, Joseph Mukhopadhyay, Supratik
...
Published in
Biomedical Optics Express
We utilized collagen specific second harmonic generation (SHG) signatures coupled with correlative immunofluorescence imaging techniques to characterize collagen structural isoforms (type I and type III) in a murine model of myocardial infarction (MI). Tissue samples were imaged over a four week period using SHG, transmitted light microscopy and im...
Akondi, Vyas Dubra, Alfredo
Published in
Biomedical Optics Express
The Shack-Hartmann wavefront sensor (SHWS) is often operated under the assumption that the sensed light can be described by a single wavefront. In biological tissues and other multi-layered samples, secondary wavefronts from axially and/or transversely displaced regions can lead to artifactual aberrations. Here, we evaluate these artifactual aberra...
Wirth, Dennis Byrd, Brook Meng, Boyu Strawbridge, Rendall R. Samkoe, Kimberley S. Davis, Scott C.
Published in
Biomedical Optics Express
Whole-animal fluorescence cryo-imaging is an established technique that enables visualization of the biodistribution of labeled drugs, contrast agents, functional reporters and cells in detail. However, many tissues produce endogenous autofluorescence, which can confound interpretation of the cryo-imaging volumes. We describe a multi-channel, hyper...
Yang, Yuhan Chen, Wei Fan, Jiang Lan Ji, Na
Published in
Biomedical Optics Express
Two-photon fluorescence microscopy has been widely applied to three-dimensional (3D) imaging of complex samples. Remote focusing by controlling the divergence of excitation light is a common approach to scanning the focus axially. However, microscope objectives induce distortion to the wavefront of non-collimated excitation beams, leading to degrad...
Kim, Tae-Hoon Le, David Son, Taeyoon Yao, Xincheng
Published in
Biomedical Optics Express
Differential artery-vein (AV) analysis is essential for retinal study, disease detection, and treatment assessment. This study is to characterize vascular reflectance profiles and blood flow patterns of retinal artery and vein systems in optical coherence tomography (OCT) and OCT angiography (OCTA), and establish them as robust signatures for objec...
Samolis, Panagis D. Langley, Daniel O’Reilly, Breanna M. Oo, Zay Hilzenrat, Geva Erramilli, Shyamsunder Sgro, Allyson E. McArthur, Sally Sander, Michelle Y.
Published in
Biomedical Optics Express
Label-free vibrational imaging of biological samples has attracted significant interest due to its integration of structural and chemical information. Vibrational infrared photothermal amplitude and phase signal (VIPPS) imaging provide label-free chemical identification by targeting the characteristic resonances of biological compounds that are pre...
Browning, Craig M. Deal, Joshua Mayes, Sam Arshad, Arslan Rich, Thomas C. Leavesley, Silas J.
Published in
Biomedical Optics Express
Colorectal cancer is the 3rd leading cancer for incidence and mortality rates. Positive treatment outcomes have been associated with early detection; however, early stage lesions have limited contrast to surrounding mucosa. A potential technology to enhance early stagise detection is hyperspectral imaging (HSI). While HSI technologies have been pre...
Peterson, Jeffrey C. Arrieta, Esdras Ruggeri, Marco Silgado, Juan D. Mintz, Keenan J. Weisson, Ernesto H. Leblanc, Roger M. Kochevar, Irene Manns, Fabrice Parel, Jean-Marie
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Published in
Biomedical Optics Express
Rose bengal photodynamic antimicrobial therapy (RB-PDAT) treats corneal infection by activating rose bengal (RB) with green light to produce singlet oxygen (1O2). Singlet oxygen dosimetry can help optimize treatment parameters. We present a 1O2 dosimeter for detection of 1O2 generated during experimental RB-PDAT. The system uses a 520 nm laser and ...
