Peralta, Xomalin G. Clary, Joseph E. Peterson, Amanda M. Noojin, Gary D. Lund, Brian J. Echeverria, Francesco J. Rockwell, Benjamin A.
Published in
Biomedical Optics Express
Lasers with ultrashort pulse durations have become ubiquitous in various applications, including ocular surgery. Therefore, we need to consider the role of nonlinear optical effects, such as supercontinuum generation during propagation within the ocular media, when evaluating their potential hazard. We used a NIR femtosecond laser to generate a sup...
Liu, Wei-Wen Chen, Sheng-Hong Li, Pai-Chi
Published in
Biomedical Optics Express
This study demonstrates that chlorophosphonazo III (CPZ III) can be used as a contrast agent for photoacoustic calcium imaging. CPZ III can pass across the plasma membrane for labeling intracellular Ca2+ without cytotoxicity. In optical-resolution photoacoustic microscopy (OR-PAM), the photoacoustic (PA) signal intensity was strongly correlated wit...
Hinsdale, Taylor A. Stallinga, Sjoerd Rieger, Bernd
Published in
Biomedical Optics Express
Structured illumination microscopy (SIM) is a widely used imaging technique that doubles the effective resolution of widefield microscopes. Most current implementations rely on diffractive elements, either gratings or programmable devices, to generate structured light patterns in the sample. These can be limited by spectral efficiency, speed, or bo...
Schleusener, Johannes Guo, Shuxia Darvin, Maxim E. Thiede, Gisela Chernavskaia, Olga Knorr, Florian Lademann, Jürgen Popp, Jürgen Bocklitz, Thomas W.
Published in
Biomedical Optics Express
Psoriasis is considered a widespread dermatological disease that can strongly affect the quality of life. Currently, the treatment is continued until the skin surface appears clinically healed. However, lesions appearing normal may contain modifications in deeper layers. To terminate the treatment too early can highly increase the risk of relapses....
Xydias, Dionysios Ziakas, Georgios Psilodimitrakopoulos, Sotiris Lemonis, Andreas Bagli, Eleni Fotsis, Theodore Gravanis, Achille Tzeranis, Dimitrios S. Stratakis, Emmanuel
Published in
Biomedical Optics Express
In this study, we use non-linear imaging microscopy to characterize the structural properties of porous collagen-GAG scaffolds (CGS) seeded with human umbilical vein endothelial cells (HUVECs), as well as human mesenchymal stem cells (hMSCs), a co-culture previously reported to form vessel-like structures inside CGS. The evolution of the resulting ...
Kratkiewicz, Karl Manwar, Rayyan Zhou, Yang Mozaffarzadeh, Moein Avanaki, Kamran
Published in
Biomedical Optics Express
Photoacoustic imaging (PAI) is an emerging functional and molecular imaging technology that has attracted much attention in the past decade. Recently, many researchers have used the vantage system from Verasonics for simultaneous ultrasound (US) and photoacoustic (PA) imaging. This was the motivation to write on the details of US/PA imaging system ...
Sommersperger, Michael Weiss, Jakob Ali Nasseri, M. Gehlbach, Peter Iordachita, Iulian Navab, Nassir
Published in
Biomedical Optics Express
The emergence of robotics could enable ophthalmic microsurgical procedures that were previously not feasible due to the precision limits of manual delivery, for example, targeted subretinal injection. Determining the distance between the needle tip, the internal limiting membrane (ILM), and the retinal pigment epithelium (RPE) both precisely and re...
Bianco, Marco Balena, Antonio Pisanello, Marco Pisano, Filippo Sileo, Leonardo Spagnolo, Barbara Montinaro, Cinzia Sabatini, Bernardo L. Vittorio, Massimo De Pisanello, Ferruccio
...
Published in
Biomedical Optics Express
As the scientific community seeks efficient optical neural interfaces with sub-cortical structures of the mouse brain, a wide set of technologies and methods is being developed to monitor cellular events through fluorescence signals generated by genetically encoded molecules. Among these technologies, tapered optical fibers (TFs) take advantage of ...
Xu, Duanyang Liang, Sijing Xu, Lin Bourdakos, Konstantinos N. Johnson, Peter Read, James Price, Jonathan H. V. Mahajan, Sumeet Richardson, David J.
Published in
Biomedical Optics Express
We demonstrate a continuous wave (CW) seeded synchronization-free optical parametric amplifier (OPA) pumped by a picosecond, 1 µm laser and show its performance when used as a simple yet powerful source for label-free coherent anti-Stokes Raman scattering (CARS), concurrent second harmonic generation (SHG), and two-photon fluorescence microscopy in...
Ikuta, Mitsuhiro Wu, Tzu-Yu Mach, Anderson T. Altshuler, Alexander Yan, Xuri Houskeeper, James H. Yamamoto, Akira Tatsumi, Shumpei Iwata, Ken-ichi Ryu, Jiheun
...
Published in
Biomedical Optics Express
Spectrally encoded endoscopy (SEE) is an ultra-miniature endoscopy technology that encodes each spatial location on the sample with a different wavelength. One challenge in SEE is achieving color imaging with a small probe. We present a novel SEE probe that is capable of conducting real-time RGB imaging using three diffraction orders (6th order dif...
