Xie, Xuhua Xu, Xiaopei Sun, Changyu Yu, Zujiang
Published in
Biological Chemistry
Hepatitis B virus X protein (HBx) played a key role in the development of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). Emerging evidence has demonstrated that miR-181b and the inhibitor of growth protein 5 (ING5) participated in the pathophysiological process. However, the regulatory mechanism of HBx remained unknown. The express...
He, Hongchao Dai, Jun Yang, Xiaoqun Wang, Xiaojing Sun, Fukang Zhu, Yu
Published in
Biological Chemistry
This study aimed to explore the effect of MED27 on the expression of epithelial-mesenchymal transition (EMT)-related proteins and β-catenin in adrenal cortical carcinoma (ACC). The functional mechanism of MED27 on ACC processes was also explored. The expression of MED27 was assessed by quantitative real-time polymerase chain reaction (qRT-PCR). siR...
Lu, Brian Munoz-Gomez, Miguel Ikeda, Yasuhiro
Published in
Biological Chemistry
Glucokinase (GCK) is crucial to regulating glucose metabolism in the liver and in pancreatic β-cells. There are two major GCK isoforms, hepatic and pancreatic GCKs, which differ only in exon 1. However, the functional differences between the two GCK isoforms remain poorly understood. Here, we used a β-cell-targeted gene transfer vector to determine...
Li, Kelei Cong, Zhe Peng, Zhuoying Chen, Ting Xue, Jing Wei, Qiang
Published in
Biological Chemistry
CD45 has been reported to regulate the HIV-1 gp120-induced apoptosis of Jurkat cells. Here, we demonstrate that the extracellular domain of CD45 plays an important role in this function. We observed that CD45RO-transfected cells, but not cells transfected with other CD45 isoforms, underwent significant apoptosis induced by gp120. However, a CD45RA-...
Drexler, Hans G. Pommerenke, Claudia Eberth, Sonja Nagel, Stefan
Published in
Biological Chemistry
Characteristic components of Hodgkin lymphoma (HL) tissue are the mono- or multinucleated Hodgkin-Reed-Sternberg (HRS) cells. Given the challenges of isolating these rare malignant cells and the difficulty in culturing cells from patients, many investigators have tried to establish cell lines in efforts to develop cellular tools for in vitro studie...
Jülicher, Karen Wähner, Annabell Haase, Kerstin Barbour, Karen W. Berger, Franklin G. Wiehlmann, Lutz Davenport, Colin Schuster-Gossler, Karin Stitz, Jörn Cantz, Tobias
...
Published in
Biological Chemistry
The generation of authentic mouse-models for human α1-antitrypsin (A1AT)-deficiency is difficult due to the high complexity of the mouse Serpina1 gene locus. Depending on the exact mouse strain, three to five paralogs are expressed, with different proteinase inhibitory properties. Nowadays with CRISPR-technology, genome editing of complex genomic l...
Liu, Siwei Lei, Huajiang Luo, Fangyuan Li, Yilin Xie, Lan
Published in
Biological Chemistry
This study aimed at investigating the biological functions of long non-coding RNAs (lncRNAs) hox transcript antisense intergenic RNA (HOTAIR) in resistant ovarian cancer cells, exploring the regulation effect of HOTAIR on HOXA7, and investigating their influence on the chemosensitivity of ovarian cancer cells. Quantitative real-time polymerase chai...
Niaz, Saife
Published in
Biological Chemistry
Small RNAs govern almost every biological process in eukaryotes associating with the Argonaute (AGO) proteins to form the RNA-induced silencing complex (mRISC). AGO proteins constitute the core of RISCs with different members having variety of protein-binding partners and biochemical properties. This review focuses on the AGO subfamily of the AGOs ...
Zhang, Libin Bu, Liang Hu, Jiang Xu, Zheyuan Ruan, Libo Fang, Yan Wang, Ping
Published in
Biological Chemistry
Non-small cell lung cancer (NSCLC) is a common malignant tumor. Although the abnormal expression and potential clinical prognostic value of histone deacetylase 1 (HDAC1) were recently discovered in many kinds of cancer, the roles and molecular mechanisms of HDAC1 in NSCLC is still limited. The CCK-8 assay is used to evaluate the viability of NSCLC ...
Milanos, Sinem Kuenzel, Katharina Gilbert, Daniel F. Janzen, Dieter Sasi, Manju Buettner, Andrea Frimurer, Thomas M. Villmann, Carmen
Published in
Biological Chemistry
GABAA receptors are ligand-gated anion channels that form pentameric arrangements of various subunits. Positive allosteric modulators of GABAA receptors have been reported as being isolated either from plants or synthesized analogs of known GABAA receptor targeting drugs. Recently, we identified monoterpenes, e.g. myrtenol as a positive allosteric ...
