Bismuth, Mike Katz, Sharon Rosenblatt, Hagar Twito, Maayan Aronovich, Ramona Ilovitsh, Tali
Published in
Bioconjugate chemistry
Noninvasive ultrasound surgery can be achieved using focused ultrasound to locally affect the targeted site without damaging intervening tissues. Mechanical ablation and histotripsy use short and intense acoustic pulses to destroy the tissue via a purely mechanical effect. Here, we show that coupled with low-frequency excitation, targeted microbubb...
Böhnke, Niels Berger, Markus Griebenow, Nils Rottmann, Antje Erkelenz, Michael Hammer, Stefanie Berndt, Sandra Günther, Judith Wengner, Antje M. Stelte-Ludwig, Beatrix
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Published in
Bioconjugate Chemistry
Inhibition of intracellular nicotinamide phosphoribosyltransferase (NAMPT) represents a new mode of action for cancer-targeting antibody–drug conjugates (ADCs) with activity also in slowly proliferating cells. To extend the repertoire of available effector chemistries, we have developed a novel structural class of NAMPT inhibitors as ADC payloads. ...
Taubel, Jillissa C Nelson, Nicholas R Bansal, Aditya Curran, Geoffrey L Wang, Lushan Wang, Zengtao Berg, Heather M Vernon, Cynthia J Min, Hoon-Ki Larson, Nicholas B
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Published in
Bioconjugate chemistry
Aberrant insulin signaling has been considered one of the risk factors for the development of Alzheimer's disease (AD) and has drawn considerable attention from the research community to further study its role in AD pathophysiology. Herein, we describe the development of an insulin-based novel positron emission tomography (PET) probe, [68Ga]Ga-NOTA...
Gerke, Christoph Zabala Gutierrez, Irene Méndez-González, Diego Cruz, M. Carmen Iglesias-de la Mulero, Francisca Jaque, Daniel Rubio-Retama, Jorge
Published in
Bioconjugate Chemistry
We present a simple methodology to design a pretargeted drug delivery system, based on clickable anti-programmed death ligand 1 (anti-PD-L1) antibodies (Abs) and clickable bovine serum albumin (BSA) nanoparticles (NPs). Pretargeted drug delivery is based on the decoupling of a targeting moiety and a drug-delivering vector which can then react in vi...
Kilic Boz, Ruveyda Aydin, Duygu Kocak, Salli Golba, Bianka Sanyal, Rana Sanyal, Amitav
Published in
Bioconjugate Chemistry
In recent years, stimuli-responsive degradation has emerged as a desirable design criterion for functional hydrogels to tune the release of encapsulated payload as well as ensure degradation of the gel upon completion of its function. Herein, redox-responsive hydrogels with a well-defined network structure were obtained using a highly efficient thi...
Yamazoe, Sayumi Kotapati, Srikanth Hogan, Jason M West, Sean M Deng, Xiaodi A Diong, S J Arbanas, Jaren Nguyen, Thien Anh Jashnani, Aarti Gupta, Diksha
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Published in
Bioconjugate chemistry
N-linked glycosylation is one of the most common and complex posttranslational modifications that govern the biological functions and physicochemical properties of therapeutic antibodies. We evaluated thermal and metabolic stabilities of antibody-drug conjugates (ADCs) with payloads attached to the C'E loop in the immunoglobulin G (IgG) Fc CH2 doma...
Hansenová Maňásková, Silvie Nazmi, Kamran Van't Hof, Wim van Belkum, Alex Kaman, Wendy E Martin, Nathaniel I Veerman, Enno C I Bikker, Floris J
Published in
Bioconjugate chemistry
Endogenous Staphylococcus aureus sortase A (SrtA) covalently incorporates cell wall anchored proteins equipped with a SrtA recognition motif (LPXTG) via a lipid II-dependent pathway into the staphylococcal peptidoglycan layer. Previously, we found that the endogenous S. aureus SrtA is able to recognize and process a variety of exogenously added syn...
Lumen, Dave Vugts, Danielle Chomet, Marion Imlimthan, Surachet Sarparanta, Mirkka Vos, Ricardo Schreurs, Maxime Verlaan, Mariska Lang, Pauline A. Hippeläinen, Eero
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Published in
Bioconjugate Chemistry
The recent advances in the production of engineered antibodies have facilitated the development and application of tailored, target-specific antibodies. Positron emission tomography (PET) of these antibody-based drug candidates can help to better understand their in vivo behavior. In this study, we report an in vivo proof-of-concept pretargeted imm...
Utterström, Johanna Barriga, Hanna M. G. Holme, Margaret N. Selegård, Robert Stevens, Molly M. Aili, Daniel
Published in
Bioconjugate Chemistry
Liposome-based drug delivery systems are widely used to improve drug pharmacokinetics but can suffer from slow and unspecific release of encapsulated drugs. Membrane-active peptides, based on sequences derived or inspired from antimicrobial peptides (AMPs), could offer means to trigger and control the release. Cholesterol is used in most liposomal ...
Battisti, Umberto M. García-Vázquez, Rocío Svatunek, Dennis Herrmann, Barbara Löffler, Andreas Mikula, Hannes Herth, Matthias Manfred
Published in
Bioconjugate Chemistry
Tetrazines (Tz) have been applied as bioorthogonal agents for various biomedical applications, including pretargeted imaging approaches. In radioimmunoimaging, pretargeting increases the target-to-background ratio while simultaneously reducing the radiation burden. We have recently reported a strategy to directly 18F-label highly reactive tetrazine...
