Chekkat, Neila Georges Dahm Chardon, Edith Wantz, May Sitz, Justine Decossas, Marion Lambert, Olivier Frisch, Benoit Rubbiani, Riccardo Gasser, Gilles
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Published in
Bioconjugate Chemistry
The current interest for platinum N-heterocyclic carbene complexes in cancer research stems from their impressive toxicity reported against a range of different human cancer cells. To date, the demonstration of their in vivo efficacy relative to that of established platinum-based drugs has not been specifically addressed. Here, we introduce an inno...
Lac, Diana Feng, Chun Bhardwaj, Gaurav Le, Huong Tran, Jimmy Xing, Li Fung, Gabriel Liu, Ruiwu Cheng, Holland Lam, Kit S
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Published in
Bioconjugate chemistry
Nonspecific ligation methods have been traditionally used to chemically modify immunoglobulins. Site-specific ligation of compounds (toxins or ligands) to antibodies has become increasingly important in the fields of therapeutic antibody-drug conjugates and bispecific antibodies. In this present study, we took advantage of the reported nucleotide-b...
Seo, Jai Woong Baek, Hyounggee Mahakian, Lisa M Kusunose, Jiro Hamzah, Juliana Ruoslahti, Erkki Ferrara, Katherine W
Published in
Bioconjugate chemistry
The ability to detect and quantify macrophage accumulation can provide important diagnostic and prognostic information for atherosclerotic plaque. We have previously shown that LyP-1, a cyclic 9-amino acid peptide, binds to p32 proteins on activated macrophages, facilitating the visualization of atherosclerotic plaque with PET. Yet, the in vivo pla...
von Maltzahn, Geoffrey Ren, Yin Park, Ji-Ho Min, Dal-Hee Kotamraju, Venkata Ramana Jayakumar, Jayanthi Fogal, Valentina Sailor, Michael J Ruoslahti, Erkki Bhatia, Sangeeta N
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Published in
Bioconjugate chemistry
The in vivo fate of nanomaterials strongly determines their biomedical efficacy. Accordingly, much effort has been invested into the development of library screening methods to select targeting ligands for a diversity of sites in vivo. Still, broad application of chemical and biological screens to the in vivo targeting of nanomaterials requires lig...
Rajesh Mukthavaram Wolfgang Wrasidlo Hall, D Santosh Kesari Milan Makale
Published in
Bioconjugate Chemistry
Quantum dots (QDs) are attracting intense interest as fluorescence labeling agents for biomedical imaging because biocompatible coatings and relatively nontoxic rare earth metal QDs have emerged as possible options. QD photoemissions are bright, of narrow wavelength range, and very stable. We sought to encapsulate QDs within targeted PEGylated lipo...
Kai Xiao Yuanpei Li Lee, Js Shi, L Tan, Yh Xing, L Holland Cheng, R Liu, Gy Kit S. Lam Luo, J
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Published in
Bioconjugate Chemistry
We have developed a well-defined and biocompatible amphiphilic telodendrimer system (PEG-b-dendritic oligo-cholic acid) which can self-assemble into multifunctional micelles in aqueous solution for efficient delivery of hydrophobic drugs such as paclitaxel. In this telodendrimer system, cholic acid is essential for the formation of stable micelles ...
Chao, Y Milan Makale Karmali, Pp Sharikov, Y Igor Tsigelny Merkulov, S Santosh Kesari Wolfgang Wrasidlo Ruoslahti, E Simberg, D
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Published in
Bioconjugate Chemistry
Dextran-coated superparamagnetic iron oxide nanoparticles (dextran-SPIO conjugates) offer the attractive possibility of enhancing MRI imaging sensitivity so that small or diffuse lesions can be detected. However, systemically injected SPIOs are rapidly removed by macrophages. We engineered embryonic cells (HEK293T) to express major macrophage scave...
Derfus, Am Chen, Aa Min, Dh Erkki Ruoslahti Bhatia, Sn
Published in
Bioconjugate Chemistry
Treatment of human diseases such as cancer generally involves the sequential use of diagnostic tools and therapeutic modalities. Multifunctional platforms combining therapeutic and diagnostic imaging functions in a single vehicle promise to change this paradigm. in particular, nanoparticle-based multifunctional platforms offer the potential to impr...
Falsey, Jr Renil, M Park, S Li, S Kit S. Lam
Published in
Bioconjugate Chemistry
A novel class of chemical microchips consisting of glass microscope slides was prepared for the covalent attachment of small molecule ligands and peptides through site-specific oxime bond or thiazolidine ring ligation reaction. Commercially available microscope slides were thoroughly cleaned and derivatized with (3-aminopropyl)triethoxysilane (APTE...
Zhao, Zg Im, Js Kit S. Lam Lake, Df
Published in
Bioconjugate Chemistry
A novel, highly specific protein modification approach is described. By using conventional molecular cloning techniques, a protein can be constructed and expressed such that the N-terminal residue is replaced by cysteine. Its 1,2-aminothiol structure reacts very specifically with a glyoxylyl group at pH 7 or below, forming a relatively stable thiaz...
