Gladyshev, Grigory V Zharova, Tatyana V Kareyeva, Alexandra V Grivennikova, Vera G
Published in
Biochimica et biophysica acta. Bioenergetics
NADH-OH, the specific inhibitor of NADH-binding site of the mammalian complex I, is shown to completely block FMN-dependent reactions of P. denitrificans enzyme in plasma membrane vesicles: NADH oxidation (in a competitive manner with Ki of 1 nM) as well as reduction of pyridine nucleotides, ferricyanide and oxygen in the reverse electron transfer....
Joliot, Pierre Sellés, Julien Wollman, Françis-André Verméglio, André
Published in
Biochimica et biophysica acta. Bioenergetics
A very high rate for cyclic electron flow (CEF) around PSI (~180 s-1 or 210 s-1 in minimum medium or in the presence of a carbon source respectively) is measured in the presence of methyl viologen (MV) in intact cells of Chlamydomonas reinhardtii under anaerobic conditions. The observation of an efficient CEF in the presence of methyl viologen is i...
Yu, Liping Fink, Brian D Som, Ritu Rauckhorst, Adam J Taylor, Eric B Sivitz, William I
Published in
Biochimica et biophysica acta. Bioenergetics
At low inner mitochondrial membrane potential (ΔΨ) oxaloacetate (OAA) accumulates in the organelles concurrently with decreased complex II-energized respiration. This is consistent with ΔΨ-dependent OAA inhibition of succinate dehydrogenase. To assess the metabolic importance of this process, we tested the hypothesis that perturbing metabolic clear...
Diuba, Artem V Vygodina, Tatiana V Azarkina, Natalia V Arutyunyan, Alexander M Soulimane, Tewfik Vos, Marten H Konstantinov, Alexander A
Published in
Biochimica et biophysica acta. Bioenergetics
Bovine cytochrome c oxidase (CcO) contains two hemes, a and a3, chemically identical but differing in coordination and spin state. The Soret absorption band of reduced aa3-type cytochrome c oxidase consists of overlapping bands of the hemes a2+ and a32+. It shows a peak at ∼444 nm and a distinct shoulder at ∼425 nm. However, attribution of individu...
Karavaeva, Val Sousa, Filipa L
Published in
Biochimica et biophysica acta. Bioenergetics
Succinate dehydrogenases (SDHs) and fumarate reductases (FRDs) catalyse the interconversion of succinate and fumarate, a reaction highly conserved in all domains of life. The current classification of SDH/FRDs is based on the structure of the membrane anchor subunits and their cofactors. It is, however, unknown whether this classification would hol...
Peters, Katharina Sargent, Frank
Published in
Biochimica et biophysica acta. Bioenergetics
Formate hydrogenlyase-1 (FHL-1) is a complex-I-like enzyme that is commonly found in gram-negative bacteria. The enzyme comprises a peripheral arm and a membrane arm but is not involved in quinone reduction. Instead, FHL-1 couples formate oxidation to the reduction of protons to molecular hydrogen (H2). Escherichia coli produces FHL-1 under ferment...
Yoon, Yisang Lee, Hakjoo Federico, Marilen Sheu, Shey-Shing
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Biochimica et biophysica acta. Bioenergetics
Mitochondrial permeability transition (MPT) is a phenomenon that the inner mitochondrial membrane (IMM) loses its selective permeability, leading to mitochondrial dysfunction and cell injury. Electrophysiological evidence indicates the presence of a mega-channel commonly called permeability transition pore (PTP) whose opening is responsible for MPT...
Agarwala, Neva Makita, Hiroki Hastings, Gary
Published in
Biochimica et biophysica acta. Bioenergetics
Time-resolved step-scan Fourier transform infrared difference spectroscopy has been used to study cyanobacterial photosystem I photosynthetic reaction centers from Synechocystis sp. PCC 6803 (S6803) with four high-potential, 1,4-naphthoquinones incorporated into the A1 binding site. The high-potential naphthoquinones are 2-chloro-, 2-bromo-, 2,3-di...
Chouhan, Nisha Yadav, Ranay Mohan Pandey, Jayendra Subramanyam, Rajagopal
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Biochimica et biophysica acta. Bioenergetics
The localization of carotenoids and macromolecular organization of thylakoid supercomplexes have not been reported yet in Chlamydomonas reinhardtii WT and cyclic electron transport mutants (pgrl1 and pgr5) under high light. Here, the various pigments, protein composition, and pigment-protein interactions were analyzed from the cells, thylakoids, an...
Svoboda, Vaclav Oung, Hui Min Olivia Koochak, Haniyeh Yarbrough, Robert Mckenzie, Steven D Puthiyaveetil, Sujith Kirchhoff, Helmut
Published in
Biochimica et biophysica acta. Bioenergetics
Knowledge about the exact abundance and ratio of photosynthetic protein complexes in thylakoid membranes is central to understanding structure-function relationships in energy conversion. Recent modeling approaches for studying light harvesting and electron transport reactions rely on quantitative information on the constituent complexes in thylako...
