Fernandez, Rebeca L Elmendorf, Laura D Smith, Robert W Bingman, Craig A Fox, Brian G Brunold, Thomas C
Published in
Biochemistry
We report the crystal structure of the mammalian non-heme iron enzyme cysteamine dioxygenase (ADO) at 1.9 Å resolution, which shows an Fe and three-histidine (3-His) active site situated at the end of a wide substrate access channel. The open approach to the active site is consistent with the recent discovery that ADO catalyzes not only the convers...
Andersen, Camilla Grønnemose, Anne Louise Pedersen, Jannik N Nowak, Jan S Christiansen, Gunna Nielsen, Janni Mulder, Frans A A Otzen, Daniel Erik Jørgensen, Thomas J D
Published in
Biochemistry
The aggregation of α-synuclein (αSN) and increased oxidative stress leading to lipid peroxidation are pathological characteristics of Parkinson's disease (PD). Here, we report that aggregation of αSN in the presence of lipid peroxidation products 4-hydroxy-2-nonenal (HNE) and 4-oxo-2-nonenal (ONE) increases the stability and the yield of αSN oligom...
Bathe, Ulschan Leong, Bryan J McCarty, Donald R Henry, Christopher S Abraham, Paul E Wilson, Mark A Hanson, Andrew D
Published in
Biochemistry
Enzymes have in vivo life spans. Analysis of life spans, i.e., lifetime totals of catalytic turnovers, suggests that nonsurvivable collateral chemical damage from the very reactions that enzymes catalyze is a common but underdiagnosed cause of enzyme death. Analysis also implies that many enzymes are moderately deficient in that their active-site r...
Wang, Kun Peng, Jinying Yi, Chengqi
Published in
Biochemistry
To date more than 150 kinds of RNA chemical modifications have been identified in cellular RNAs, among which N6-methyladenosine (m6A) is the most prevalent mRNA modification in higher eukaryotes. m6A is widely conserved among eukaryotes and tends to occur in a RRACH consensus motif. This consensus motif is identified as early as the 1970s and posit...
Dyson, H Jane
Published in
Biochemistry
The study of protein dynamics using the measurement of relaxation times by NMR was based on a set of studies in the mid-20th century that outlined theories and methods. However, the complexity of protein NMR was such that these simple experiments were not practical for application to proteins. The advent of techniques in the 1980s for isotopic labe...
Yang, Dongsoo Zhou, Hengrui Lee, Sang Yup
Published in
Biochemistry
Lewis, Julia K Jochimsen, Andrew S Lefave, Sarah J Young, Anthony P Kincannon, William M Roberts, Andrew G Kieber-Emmons, Matthew T Bandarian, Vahe
Published in
Biochemistry
Ribosomally synthesized post-translationally modified peptides (RiPPs) are ubiquitous and represent a structurally diverse class of natural products. The ribosomally encoded precursor polypeptides are often extensively modified post-translationally by enzymes that are encoded by coclustered genes. Radical S-adenosyl-l-methionine (SAM) enzymes catal...
Brown, Janae B Lee, Mark A Smith, Aaron T
Published in
Biochemistry
Iron is an essential nutrient for virtually every living organism, especially pathogenic prokaryotes. Despite its importance, however, both the acquisition and the export of this element require dedicated pathways that are dependent on oxidation state. Due to its solubility and kinetic lability, reduced ferrous iron (Fe2+) is useful to bacteria for...
Qian, Pu Swainsbury, David J K Croll, Tristan I Castro-Hartmann, Pablo Divitini, Giorgio Sader, Kasim Hunter, C Neil
Published in
Biochemistry
Light-harvesting 2 (LH2) antenna complexes augment the collection of solar energy in many phototrophic bacteria. Despite its frequent role as a model for such complexes, there has been no three-dimensional (3D) structure available for the LH2 from the purple phototroph Rhodobacter sphaeroides. We used cryo-electron microscopy (cryo-EM) to determine...
Preußke, Nils Lipfert, Matthias Rothemund, Sven Leippe, Matthias Sönnichsen, Frank D
Published in
Biochemistry
α-Helical antimicrobial peptides (αAMPs) are among the potential candidates for new anti-infectives to tackle the global crisis in antibiotic resistance, but they suffer from low bioavailability due to high susceptibility to enzymatic degradation. Here, we describe a strategy to increase the resistance of αAMPs against proteases. Fusing the 12-resi...
