Zhu, Bo Qian, Cheng Tang, Haoxuan Kitaguchi, Tetsuya Ueda, Hiroshi
Published in
Biochemistry
Escherichia coli β-glucuronidase (GUS) has been used as a reporter enzyme in molecular biology and engineered as an enzyme switch for the development of homogeneous biosensors. In this study, we developed a thermostable GUS enzyme switch based on the thermostable GUS mutant TR3337 by disrupting a conserved salt bridge (H514-E523) between the diagon...
Steiner, Paul J Swift, Samuel D Bedewitz, Matthew Wheeldon, Ian Cutler, Sean R Nusinow, Dmitri A Whitehead, Timothy A
Published in
Biochemistry
Chemical-induced dimerization (CID) modules enable users to implement ligand-controlled cellular and biochemical functions for a number of problems in basic and applied biology. A special class of CID modules occur naturally in plants and involve a hormone receptor that binds a hormone, triggering a conformational change in the receptor that enable...
Tyagi, Jaya Lakshmi Sharma, Meenakshi Gulati, Khushboo Kairamkonda, Manikyaprabhu Kumar, Dinesh Poluri, Krishna Mohan
Published in
Biochemistry
The therapeutic use of bacteriophage-encoded endolysins as enzybiotics has increased significantly in recent years due to the emergence of antibiotic resistant bacteria. Phage endolysins lyse the bacteria by targeting their cell wall. Various engineering strategies are commonly used to modulate or enhance the utility of therapeutic enzymes. This st...
Sun, Li-Juan Yuan, Hong Xu, Jia-Kun Luo, Jie Lang, Jia-Jia Wen, Ge-Bo Tan, Xiangshi Lin, Ying-Wu
Published in
Biochemistry
The design of functional metalloenzymes is attractive for the biosynthesis of biologically important compounds, such as phenoxazinones and phenazines catalyzed by native phenoxazinone synthase (PHS). To design functional heme enzymes, we used myoglobin (Mb) as a model protein and introduced an artificial CXXC motif into the heme distal pocket by F4...
Yadav, Yogita Barman, Soumen Roy, Anirban Padhan, Jyotirmayee Sudhamalla, Babu
Published in
Biochemistry
Combinatorial readout of histone post-translational modifications by tandem reader modules mediates crosstalk among different histone modifications. To identify the domain-specific interactome of the tandem reader, we engineered the dual bromodomain of TATA-binding protein-associated factor-1 (TAF1) to carry a photoactivatable unnatural amino acid,...
Matsumura, Ichiro Patrick, Wayne M
Published in
Biochemistry
Natural evolution has been creating new complex systems for billions of years. The process is spontaneous and requires neither intelligence nor moral purpose but is nevertheless difficult to understand. The late Dan Tawfik spent years studying enzymes as they adapted to recognize new substrates. Much of his work focused on gaining fundamental insig...
Daniels, Page N van der Donk, Wilfred A
Published in
Biochemistry
The preparation of protein-protein, protein-peptide, and protein-small molecule conjugates is important for a variety of applications, such as vaccine production, immunotherapies, preparation of antibody-drug conjugates, and targeted delivery of therapeutics. To achieve site-selective conjugation, selective chemical or enzymatic functionalization o...
Joho, Yvonne Vongsouthi, Vanessa Spence, Matthew A Ton, Jennifer Gomez, Chloe Tan, Li Lynn Kaczmarski, Joe A Caputo, Alessandro T Royan, Santana Jackson, Colin J
...
Published in
Biochemistry
The improved production, recycling, and removal of plastic waste, such as polyethylene terephthalate (PET), are pressing environmental and economic issues for society. Biocatalytic (enzymatic) PET depolymerization is potentially a sustainable, low-energy solution to PET recycling, especially when compared with current disposal methods such as landf...
Papadopoulou, Athena Meyer, Fabian Buller, Rebecca M
Published in
Biochemistry
Fe(II)/α-ketoglutarate-dependent dioxygenases (α-KGDs) are widespread enzymes in aerobic biology and serve a remarkable array of biological functions, including roles in collagen biosynthesis, plant and animal development, transcriptional regulation, nucleic acid modification, and secondary metabolite biosynthesis. This functional diversity is refl...
Way, Jeffrey C Burrill, Devin R Silver, Pamela A
Published in
Biochemistry
Natural systems use weak interactions and avidity effects to give biological systems high specificity and signal-to-noise ratios. Here we describe design principles for engineering fusion proteins that target therapeutic fusion proteins to membrane-bound signaling receptors by first binding to designer-chosen co-receptors on the same cell surface. ...
