Kuzmin, Kiril Adeniyi, Ayotomiwa Ezekiel DaSouza, Arthur Kevin Jr. Lim, Deuk Nguyen, Huyen Molina, Nuria Ramirez Xiong, Lanqiao Weber, Irene T. Harrison, Robert W.
Published in
Biochemical and Biophysical Research Communications
• Coronavirus spike protein sequences can predict host specificity. • Machine learning had accuracies of >0.98 for classification of human vs non-human hosts. • Decision Tree classifier identified spike regions with important biological roles . • Clustering with t -SNE embeddings correctly segregated sequences by virus genus and host .
Qiao, Jialu Li, Weiling Bao, Jian Peng, Qian Wen, Dongmei Wang, Jianing Sun, Binlian
Published in
Biochemical and Biophysical Research Communications
• ACE2 is lower expressed in mostly brain cell lines and tissues. • CD147 has a higher level of expression in brain cells lines and tissues. • TMPRSS2 has consistent expression in brain cells lines and tissues. • The cerebral nervous system is more likely to be infected with SARS-CoV-2 through CD147 receptor and protease TMPRSS2. • With reference t...
Jeremiah, Sundararaj S. Miyakawa, Kei Morita, Takeshi Yamaoka, Yutaro Ryo, Akihide
Published in
Biochemical and Biophysical Research Communications
• Silver nanoparticles inhibit extracellular SARS-CoV-2. • 2–15 nm size particles showed robust inhibition of SARS-CoV-2. • SARS-CoV-2 was potently inhibited at concentrations above 1 ppm. • Naked Silver nanoparticles exhibited cytotoxicity from 20 ppm onwards.
Jiang, Yi Yin, Wanchao Xu, H. Eric
Published in
Biochemical and Biophysical Research Communications
Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has rapidly become a global pandemic. Although great efforts have been made to develop effective therapeutic interventions, only the nucleotide analog remdesivir was approved for emergency use against COVID-19. Remdesivir targets the RNA-depen...
Du, Zhen Chakrabarti, Sampurna Kulaberoglu, Yavuz Smith, Ewan St John Dobson, Christopher M. Itzhaki, Laura S. Kumita, Janet R.
Published in
Biochemical and Biophysical Research Communications
• Healthy ageing in the naked mole-rat (NMR) may be linked to a robust proteostasis. • Currently, NMR studies heavily rely on protein-level analysis of proteostasis. • We report transcriptional assays probing the NMRs unfolded protein response (UPR). • Distinct responses in NMR UPR observed between mild and severe ER stress induction. • Our assays ...
Shi, Tzu-Hau Huang, Yi-Long Chen, Chiao-Che Pi, Wen-Chieh Hsu, Yu-Ling Lo, Lee-Chiang Chen, Wei-Yi Fu, Shu-Ling Lin, Chao-Hsiung
Published in
Biochemical and Biophysical Research Communications
• 2019-nCoV and SARS-CoV main proteases exhibit similar enzyme kinetics. • Andrographolide inhibits enzyme activity of 2019-nCoV and SARS-CoV main proteases. • Andrographolide is covalently linked to active cysteine of 2019-nCoV main protease.
Tung, H.Y. Lim Limtung, Pierre
Published in
Biochemical and Biophysical Research Communications
• Mutations in the phosphorylation sites of SARS-CoV-2 encoded nucleocapsid protein. • Phosphorylation dependent binding and sequestration sites for protein 14-3-3. • Structure-model of the phosphorylation dependent binding and sequestration. • Proposal that sequestration by Protein 14-3-3 is a cellular response mechanism. • Sequestration inhibits ...
Chen, Peng Zeng, Zehua Du, Hongwu
Published in
Biochemical and Biophysical Research Communications
• This study first proposed the idea of SARS-CoV-2 specific protein target microarray. • The microarray was established and validated by practical test. • Network pharmacology suggest this chip has a broad application in pharmacology.
Takeda, Yohei Uchiumi, Hiroshi Matsuda, Sachiko Ogawa, Haruko
Published in
Biochemical and Biophysical Research Communications
• Acidic electrolyzed water (EW) shows virucidal activity against SARS-CoV-2. • Virucidal activity of acidic EW depends on free available chlorine (FAC). • Acidic solution without FAC does not inactivate SARS-CoV-2 in a 1-min reaction. • Large amounts of FAC are required to inactivate virus containing many proteins.
Singh Tomar, Prabhat Pratap Arkin, Isaiah T.
Published in
Biochemical and Biophysical Research Communications
• We have shown that SARS-CoV-2 E protein is a potential ion channel. • Ion channels are excellent targets for pharmaceutical inhibition. • Screening a small library of blockers yielded Gliclazide and Memantine that inhibit SARS-CoV-2 E protein’s channel activity. • Our results show that we have an excellent target to curb SARS-CoV-2 as a route to ...
