Published in Biochemical and Biophysical Research Communications
• RNA stability is an important regulator for viral life-cycle. • Fate-seq enables measurement of stabilization activity of RNAs derived from viruses. • Conserved sequences among coronaviruses including SARS-CoV-2 have stabilizing activity.
Published in Biochemical and Biophysical Research Communications
• SARS-CoV-2 nucleocapsid protein is full of coils and highly disordered. • SARS-CoV-2 N protein forms a dimer by CTD-CTD interaction. • SARS-CoV-2 N protein can bind with non-specific nucleic acid with high affinity. • The presence of IgA, IgM and IgG antibodies against the N protein were detected in the confirmed COVID-19 patients' sera pool.
Published in Biochemical and Biophysical Research Communications
• Some bat cell lines showed a limited cytopathic effect after EMCV and JEV infection. • EMCV and JEV replication was inhibited in some bat cell lines. • Expression of PRRs and IFN-β was highly induced in some bat cell lines. • PRRs and IFN-β contributed to antiviral response against EMCV and JEV in bats.
Published in Biochemical and Biophysical Research Communications
• Roscovitine possesses antiviral activities against different subtypes of influenza A viruses. • Roscovitine suppresses the gene transcription and genome replication steps in the viral life cycle. • Roscovitine exerts its anti-influenza activity through specific binding to the PB2cap protein.
Published in Biochemical and Biophysical Research Communications
• Search for ligand binding sites in COVID-19 S glycoprotein protomeric interfaces. • Structure based ligand selection for interfering to COVID-19 S glycoprotein assembly. • Ligand-protomer docking simulation to find the best candidates for therapeutic use.
Published in Biochemical and Biophysical Research Communications
• ACE2 is activated by SARS-CoV-2 infection. • ACE2 is enriched in human preimplantation embryos and germ cells. • EZH2 represses ACE2 expression in human embryonic stem cells. • EZH2 mediates H3K27me3 at human ACE2 promoter region.
Published in Biochemical and Biophysical Research Communications
• Remdesivir attenuates metabolic syndrome in HFD-fed mice. • Remdesivir alleviates hepatic function and lipid accumulation in HFD-induced mice. • Remdesivir inhibits inflammatory response in liver of HFD-fed mice. • Remdesivir-regulated lipid accumulation and inflammation is dependent on STING in PA-incubated hepatocytes.
Published in Biochemical and Biophysical Research Communications
• Pets (dog and cat), pangolin and Circetidae remained the key residues for association with S from SARS-CoV and SARS-CoV-2. • The interface of the interaction between cat/dog/pangolin/Chinese hamster ACE2 and SARS-CoV/SARS-CoV-2 RBD was simulated. • N82 of ACE2 showed a closer contact with SARS-CoV-2 S than M82, suggesting an optimized ACE2 for SA...
Published in Biochemical and Biophysical Research Communications
• ACE2 is expressed in lung, liver, stomach, ileum, kidney and colon. • ANPEP, DPP4 and ENPEP were identified as the candidate co-receptors, showing the most similar expression patterns with ACE2. • Macrophages frequently communicate with the CoVs targets through chemokine and phagocytosis signaling.
Published in Biochemical and Biophysical Research Communications
• Ionic gate controls current flow in closed state, gate opens when ATP binds. • MTSET labelling suggests desensitisation gate in different place than closed state. • T18A affects desensitisation by disrupting a conserved hydrogen bond network.
