Oates, Andrew C. Wollberg, Patrik Achen, Marc G. Wilks, Andrew F.
Published in
Biochemical and Biophysical Research Communications
The polymerase chain reaction (PCR), with cDNA as template, has been widely used to identify members of protein families from many species. A major limitation of using cDNA in PCR is that detection of a family member is dependent on temporal and spatial patterns of gene expression. To circumvent this restriction, and in order to develop a technique...
Wang, Baojin Shen, Airong Ouyang, Xuan Zhao, Guannan Du, Ziyun Huo, Wenying Zhang, Tao Wang, Yinan Yang, Chuanhe Dong, Peixin
...
Published in
Biochemical and biophysical research communications
KLF4 is a transcriptional factor that can function either as a tumor suppressor or oncogene in cancer based on its cellular context. We recently demonstrated that KLF4 was a tumor suppressor in ovarian cancer cells by inhibiting the epithelial to mesenchymal transition. Here we report that KLF4 expression was downregulated in ovarian cancer tissue ...
Nayak, Sunil Bryant, Floyd R
Published in
Biochemical and Biophysical Research Communications
The kinetics of the ATP and dATP-mediated formation of a functionally-active RecA-ssDNA complex were examined by stopped-flow fluorescence spectroscopy, using a modified version of the RecA protein that contains a fluorescent reporter group in the ssDNA binding site. The results indicated that: i) an active RecA-ssDNA complex was formed more rapidl...
Yoon, Hyun Kyung Sohn, Kyung-Cheol Lee, Jung-Suk Kim, Yu Jin Bhak, Jong Yang, Jun-Mo You, Kwan-Hee Kim, Chang-Deok Lee, Jeung-Hoon
Published in
Biochemical and Biophysical Research Communications
Terminal differentiation of skin keratinocytes is a vertically directed multi-step process that is tightly controlled by the sequential expression of a variety of genes. We previously investigated the gene expression profile and found that many of differentiation-related genes expressed in a temporally regulated manner. In this study, we attempted ...
Cheng, Ping Dai, Weiqi Wang, Fan Lu, Jie Shen, Miao Chen, Kan Li, Jingjing Zhang, Yan Wang, Chengfen Yang, Jing
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Published in
Biochemical and Biophysical Research Communications
Ethyl pyruvate (EP) was recently identified as a stable lipophilic derivative of pyruvic acid with significant antineoplastic activities. The high mobility group box-B1 (HMGB1)–receptor for advanced glycation end-products (RAGE) and the protein kinase B (Akt) pathways play a crucial role in tumorigenesis and development of many malignant tumors. We...
Zhang, Guangjun Zhou, He Xiao, Huaxu Liu, Zuoliang Tian, Hongpeng Zhou, Tong
Published in
Digestive Diseases and Sciences
BackgroundOur previous studies show that microRNA-92a (miR-92a) is overexpressed in colorectal cancer (CRC) and is thought to be correlated with the development of the cancer. However, its biological role in CRC remains poorly understood.AimsThe aim of the study was to determine the role of miR-92a and to elucidate its regulatory mechanism in CRC.M...
Wang, Feng Yang, Yong
Published in
Biochemical and biophysical research communications
Polyhistidine is widely used for the delivery of nucleic acids and antibodies into the cell cytoplasm. However, little attention has been concerned on the effect of polyhistidine on the immune system. In this work, we identify a novel function of polyhistidine as an activator of the immune system. Single-molecule fluorescence imaging and single-mol...
Wang, Feng Yang, Yong
Published in
Biochemical and biophysical research communications
Although the flavonoid quercetin is known to inhibit activation of insulin receptor signaling, the inhibitory mechanism is largely unknown. In this study, we demonstrate that quercetin suppresses insulin induced dimerization of the insulin receptor (IR) through interfering with ligand-receptor interactions, which reduces the phosphorylation of IR a...
Ali, Haytham Galal, Omima Urata, Yoshishige Goto, Shinji Guo, Chang-Ying Luo, Lan Abdelrahim, Eman Ono, Yusuke Mostafa, Emtethal Li, Tao-Sheng
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Published in
Biochemical and Biophysical Research Communications
Nicaraven, a hydroxyl radical-specific scavenger has been demonstrated to attenuate radiation injury in hematopoietic stem cells with 5Gy γ-ray exposures. We explored the effect and related mechanisms of nicaraven for protecting radiation injury induced by sequential exposures to a relatively lower dose γ-ray. C57BL/6 mice were given nicaraven or p...
Jung, Sujin Kang, Jeong Gu Lee, Ju Hee Song, Kyoung Jin Ko, Jeong-Heon Kim, Yong-Sam
Published in
Biochemical and Biophysical Research Communications
Contact inhibition has been largely elusive despite that a loss of contact inhibition is a critical event for cancer development and progression. Here, we report that PHLPP1 is a binding protein for Mst1 and it modulates the Hippo pathway by dephosphorylating Mst1 at the inhibitory Thr387 of Mst1. Yap1 was localized predominantly in the nucleus but...
