Tahara, Makiko Inoue, Takeshi Miyakura, Yasuyuki Horie, Hisanaga Yasuda, Yoshikazu Fujii, Hirofumi Kotake, Kenjiro Sugano, Kokichi
Published in
Biochemical and Biophysical Research Communications
In vitro assessment of chemosensitivity are important for experiments evaluating cancer therapies. The Scepter 2.0 cell counter, an automated handheld device based on the Coulter principle of impedance-based particle detection, enables the accurate discrimination of cell populations according to cell size and volume. In this study, the effects of S...
Li, Yigen Huang, Wenjin Huang, Shenyuan Du, Jiulin Huang, Cheng
Published in
Biochemical and Biophysical Research Communications
The MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide) assay is a classical method for screening cytotoxic anti-cancer agents. Candidate drugs from the MTT assay need in vivo models to test their efficiency and to assess the absorption, distribution, metabolism, excretion, and toxicity of the drugs. An in vivo screening model could ...
Pasetto, Damiano Lemaitre, Joseph C. Bertuzzo, Enrico Gatto, Marino Rinaldo, Andrea
Published in
Biochemical and Biophysical Research Communications
To monitor local and global COVID-19 outbreaks, and to plan containment measures, accessible and comprehensible decision-making tools need to be based on the growth rates of new confirmed infections, hospitalization or case fatality rates. Growth rates of new cases form the empirical basis for estimates of a variety of reproduction numbers, dimensi...
Pereira, Filipe
Published in
Biochemical and Biophysical Research Communications
The SARS-CoV-2 Variant of Concern 202012/01 (VOC-202012/01) emerged in southeast England and rapidly spread worldwide. This variant is believed to be more transmissible, with all attention being given to its spike mutations. However, VOC-202012/01 has also a mutation (Q27stop) that truncates the ORF8, a likely immune evasion protein. Removal of ORF...
Jang, Minsu Park, Rackhyun Park, Yea-In Cha, Yeo-Eun Yamamoto, Ayane Lee, Jin I. Park, Junsoo
Published in
Biochemical and Biophysical Research Communications
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Duarte, Carolina Akkaoui, Juliet Ho, Anny Garcia, Christopher Yamada, Chiaki Movila, Alexandru
Published in
Biochemical and Biophysical Research Communications
The SARS-CoV-2 virus causes elevated production of senescence-associated secretory phenotype (SASP) markers by macrophages. SARS-CoV-2 enters macrophages through its Spike-protein aided by cathepsin (Cat) B and L, which also mediate SASP production. Since M-CSF and IL-34 control macrophage differentiation, we investigated the age-dependent effects ...
Yuan, Meng Liu, Hejun Wu, Nicholas C. Wilson, Ian A.
Published in
Biochemical and Biophysical Research Communications
• The receptor binding domain is a major target of antibodies to SARS-CoV-2. • Neutralizing antibodies target various epitopes on the receptor binding domain. • Convergent antibody responses to SARS-CoV-2 are observed. • Cross-neutralization of SARS-CoV-2 and SARS-CoV maps to two RBD epitopes. • Antibody avidity can be crucial for neutralization po...
Sakurai, Yasuteru Ngwe Tun, Mya Myat Kurosaki, Yohei Sakura, Takaya Inaoka, Daniel Ken Fujine, Kiyotaka Kita, Kiyoshi Morita, Kouichi Yasuda, Jiro
Published in
Biochemical and Biophysical Research Communications
The current COVID-19 pandemic requires urgent development of effective therapeutics. 5-amino levulinic acid (5-ALA) is a naturally synthesized amino acid and has been used for multiple purposes including as an anticancer therapy and as a dietary supplement due to its high bioavailability. In this study, we demonstrated that 5-ALA treatment potently...
Chakraborty, Sandipan
Published in
Biochemical and Biophysical Research Communications
The recognition of ACE2 by the receptor-binding domain (RBD) of spike protein mediates host cell entry. The objective of the work is to identify SARS-CoV2 spike variants that emerged during the pandemic and evaluate their binding affinity with ACE2. Evolutionary analysis of 2178 SARS-CoV2 genomes identifies RBD variants that are under selection bia...
Intawong, Kannikar Olson, Debra Chariyalertsak, Suwat
Published in
Biochemical and Biophysical Research Communications
Demands to address the COVID-19 pandemic rapidly surpassed global resources. Successful implementation of application technology resulting in people taking greater control of their own health and medical and public health personnel improving efficiency was requested by authorities in Thailand to reduce the demand on health resources to meet the hea...
