Published in BBA - Protein Structure
Pregnant mare serum gonadotropin exhibits a dissociation at acid pH as shown by the drop of s 20,w values from 3.52 S at pH 8.1 to 2.52 S at pH 2.0. The dissociation is accompanied by an absorbance change with a maximum at 287 nm and a parallel loss of both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) activities as followed by ra...
Published in BBA - Protein Structure
A new rat serum protein has been isolated by affinity chromatography using ethanolamine- or phosphoethanolamine-substituted agarose gels. This protein has the morphological and functional characteristics of serum amyloid P-component and C-reactive protein. It comprises C 5 cyclic symmetry structure with non covalently cross-linked subunits which ha...
Published in BBA - Protein Structure
The first component of complement (C1q) coupled to Sepharose by cyanogen bromide was found not to bind aggregated human γ-globulin or immune complexes at room temperature, whereas at 4°C binding was nearly complete. The temperature sensitivity of solid phase C1q binding was reversible. Elution of aggregated human γ-globulin bound at 4°C was possibl...
Published in BBA - Protein Structure
The fast step in the conformational change of human serum albumin from the alkaline to the neutral form of the albumin-bilirubin complex is studied by various pH jump experiments in a stopped-flow apparatus. The results indicate that the fast step is caused by electrostatic attraction between a carboxylate group of bilirubin and a histidine residue...
Published in BBA - Protein Structure
Magnetic (MCD) and natural circular dichroism (CD) spectra of various complexes of cytochrome P-450 11 β ( P-450 11 β ) and cytochrome P-450 scc ( P-450 scc) from bovine adrenal cortex were measured from 250 nm to 700 nm. MCD and CD spectral contours of cytochromes P-450 11 β and P-450 scc in the Soret and visible regions were, as a whole, analogou...
Published in BBA - Protein Structure
The secondary structure of chicken egg white ovomucoid and its domains was studied by means of circular dichroism (CD). The various domains (domain I, domains II · III, domains I · II, and domain III with and without carbohydrate) were prepared from the ovomucoid by cyanogen bromide cleavage and Staphylococcus aureus protease V-8 digestions. The ca...
Published in BBA - Protein Structure
The pentapeptide Arg-Lys-Asp-Val-Tyr (TP5) is a biologically active fragment of thymopoietin, the thymic hormone that induces selective T-cell differentiation. The formation of lanthanide(III) complexes of TP5 is demonstrated through the observation of Tb 3+ fluorescence enhancement. The equilibria, stoichiometry and solution conformation of the La...
Published in BBA - Protein Structure
A new technique for surface labeling of biological structures based on dye-sensitized photochemical coupling of labeled low molecular weight substances has been applied to studies on fibrinogen. When a solution of fibrinogen containing fluorescein and 3H-labeled tryptophan was illuminated with visible light, radioactive labeling of fibrinogen occur...
Published in BBA - Protein Structure
Published in BBA - Protein Structure
