McMorran, Lindsay M. Brockwell, David J. Radford, Sheena E.
Published in
Archives of Biochemistry and Biophysics
Research into the mechanisms by which proteins fold into their native structures has been on-going since the work of Anfinsen in the 1960s. Since that time, the folding mechanisms of small, water-soluble proteins have been well characterised. By contrast, progress in understanding the biogenesis and folding mechanisms of integral membrane proteins ...
Yan, Libo Cai, Qingchun Xu, Yan
Published in
Archives of biochemistry and biophysics
The Hippo-YAP pathway is altered and implicated as an oncogenic signaling pathway in many human cancers. Hypoxia is an important microenvironmental factor that promotes tumorigenesis. However, the effects of hypoxia on the two most important Hippo-YAP effectors, YAP (Yes-associated protein) and TAZ (transcriptional co-activator with PDZ-binding mot...
Cappariello, Alfredo Maurizi, Antonio Veeriah, Vimal Teti, Anna
Published in
Archives of biochemistry and biophysics
Much has been written recently on osteoclast biology, but this cell type still astonishes scientists with its multifaceted functions and unique properties. The last three decades have seen a change in thinking about the osteoclast, from a cell with a single function, which just destroys the tissue it belongs to, to an "orchestrator" implicated in t...
Ruotolo, Roberta Calani, Luca Brighenti, Furio Crozier, Alan Ottonello, Simone Del Rio, Daniele
Published in
Archives of Biochemistry and Biophysics
Several plant-derived molecules, referred to as phytoestrogens, are thought to mimic the actions of endogenous estrogens. Among these, quercetin, one of the most widespread flavonoids in the plant kingdom, has been reported as estrogenic in some occasions. However, quercetin occurs in substantial amounts as glycosides such as quercetin-3-O-glucosid...
Cappariello, Alfredo Maurizi, Antonio Veeriah, Vimal Teti, Anna
Published in
Archives of biochemistry and biophysics
Much has been written recently on osteoclast biology, but this cell type still astonishes scientists with its multifaceted functions and unique properties. The last three decades have seen a change in thinking about the osteoclast, from a cell with a single function, which just destroys the tissue it belongs to, to an "orchestrator" implicated in t...
Chung, Charles S. Mitov, Mihail I. Callahan, Leigh Ann Campbell, Kenneth S.
Published in
Archives of Biochemistry and Biophysics
Diastolic dysfunction is a clinically significant problem for patients with diabetes and often reflects increased ventricular stiffness. Attached cross-bridges contribute to myocardial stiffness and produce short-range forces, but it is not yet known whether these forces are altered in diabetes. In this study, we tested the hypothesis that cross-br...
Jackson, Del R. Jr. Webb, Milad Stewart, Travis J. Phillips, Travis Carter, Michael Cremo, Christine R. Baker, Josh E.
Published in
Archives of Biochemistry and Biophysics
To determine the mechanism by which sucrose slows in vitro actin sliding velocities, V, we used stopped flow kinetics and a single molecule binding assay, SiMBA. We observed that in the absence of ATP, sucrose (880mM) slowed the rate of actin–myosin (A–M) strong binding by 71±8% with a smaller inhibitory effect observed on spontaneous rigor dissoci...
Karpicheva, Olga E. Redwood, Charles S. Borovikov, Yurii S.
Published in
Archives of Biochemistry and Biophysics
The effect of the skeletal myopathy-causing E117K mutation in human β-tropomyosin on actomyosin structure during the ATPase cycle was studied using fluorescent probes bound to actin subdomain 1 and the myosin head. Multistep changes in flexural rigidity of the filament and in spatial arrangement of actin subdomain 1 and myosin SH1 helix in troponin...
Mikles, David C. Schuchardt, Brett J. Bhat, Vikas McDonald, Caleb B. Farooq, Amjad
Published in
Archives of Biochemistry and Biophysics
In response to a wide variety of stimuli such as growth factors and hormones, EGR1 transcription factor is rapidly induced and immediately exerts downstream effects central to the maintenance of cellular homeostasis. Herein, our biophysical analysis reveals that DNA sequence variations within the target gene promoters tightly modulate the energetic...
Flemmig, Jörg Rusch, Dorothea Czerwińska, Monika Ewa Rauwald, Hans-Wilhelm Arnhold, Jürgen
Published in
Archives of Biochemistry and Biophysics
We investigated in vitro the ability of a standardised olive leaf dry extract (Ph. Eur.) (OLE) as well as of its single components to circumvent the hydrogen peroxide-induced inhibition of the hypothiocyanite-producing activity of lactoperoxidase (LPO). The rate of hypothiocyanite (−OSCN) formation by LPO was quantified by spectrophotometric detect...
