Komorizono, Ryo Yoshizumi, Shima Tomonaga, Keizo
Published in
Applied microbiology and biotechnology
RNA virus-based episomal vector (REVec), engineered from Borna disease virus, is an innovative gene delivery tool that enables sustained gene expression in transduced cells. However, the difficulty in controlling gene expression and eliminating vectors has limited the practical use of REVec. In this study, we overcome these shortcomings by insertin...
Tan, Zhijie Chen, Wenli Guo, Ziyu Xu, Xingyuan Xie, Junting Dai, Jiangpeng Lin, Yuexia Sheng, Binbin Preis, Sergei Wei, Chaohai
...
Published in
Applied microbiology and biotechnology
Seasonal dynamics of bacterial composition and functions were demonstrated for the biological fluidized-bed bioreactors combined in the anoxic/aerobic1/aerobic2 (AOO) coking wastewater (CWW) treatment sequences. The bacterial composition and functions in the CWW activated sludge samples were revealed by 16S rRNA genes amplicon sequencing. Thiobacil...
Zheng, Caijuan Hou, Shuxin Zhou, Yu Yu, Changyuan Li, Hao
Published in
Applied microbiology and biotechnology
Saccharomyces cerevisiae is a widely used strain for ethanol fermentation; meanwhile, efficient utilization of glucose could effectively promote ethanol production. The PFK1 gene is a key gene for intracellular glucose metabolism in S. cerevisiae. Our previous work suggested that although deletion of the PFK1 gene could confer higher oxidative tole...
Fekete-Kertész, Ildikó Berkl, Zsófia Buda, Kata Fenyvesi, Éva Szente, Lajos Molnár, Mónika
Published in
Applied microbiology and biotechnology
Various virulence determinants in Pseudomonas aeruginosa are regulated by the quorum sensing (QS) network producing and releasing signalling molecules. Two of these virulence determinants are the pyocyanin and pyoverdine, which interfere with multiple cellular functions during infection. The application of QS-inhibiting agents, such as cyclodextrin...
Rasul, Faiz You, Dawei Jiang, Ying Liu, Xiangjian Daroch, Maurycy
Published in
Applied microbiology and biotechnology
Thermophilic cyanobacteria are prokaryotic photoautotrophic microorganisms capable of growth between 45 and 73 °C. They are typically found in hot springs where they serve as essential primary producers. Several key features make these robust photosynthetic microbes biotechnologically relevant. These are highly stable proteins and their complexes, ...
Kastenhofer, Jens Spadiut, Oliver Papangelakis, Vladimiros G Allen, D Grant
Published in
Applied microbiology and biotechnology
The increasing demand for rare earth elements (REEs) has spurred interest in the development of recovery methods from aqueous waste streams. Acidophilic microalgae have gained attention for REE biosorption as they can withstand high concentrations of transition metals and do not require added organic carbon to grow, potentially allowing simultaneou...
Lei, Eric K Azmat, Aruba Henry, Kevin A Hussack, Greg
Published in
Applied microbiology and biotechnology
Bacterial outer membrane vesicles (OMVs) are nanosized spheroidal particles shed by gram-negative bacteria that contain biomolecules derived from the periplasmic space, the bacterial outer membrane, and possibly other compartments. OMVs can be purified from bacterial culture supernatants, and by genetically manipulating the bacterial cells that pro...
Sindarovska, Yana Kuchuk, Mykola
Published in
Applied microbiology and biotechnology
Human interferon (hINF) alpha 2b is clinically important pharmaceutical product included in combinatory therapy against chronic hepatitis C and B and complex therapy against several cancer diseases. Here, we created the genetic constructions, based on genome elements of potato virus X (PVX), carrying the infα2b gene for transient expression in plan...
Sánchez-Arroyo, Ana Plaza-Vinuesa, Laura Abeijón-Mukdsi, María Claudia de Las Rivas, Blanca Mancheño, José Miguel Muñoz, Rosario
Published in
Applied microbiology and biotechnology
The presence of ochratoxin A (OTA) in food and feed represents a serious concern since it raises severe health implications. Bacterial strains of the Acinetobacter genus hydrolyse the amide bond of OTA yielding non-toxic OTα and L-β-phenylalanine; in particular, the carboxypeptidase PJ15_1540 from Acinetobacter sp. neg1 has been identified as an OT...
Zhang, Furui Shi, Tianyi Zhang, Zhen Wang, Shue Liu, Jing Li, Yonghong Wang, Xuequan Liu, Kunmei Guo, Le
Published in
Applied microbiology and biotechnology
The acidic environment and enzyme degradation lead to oral vaccines often having little immune effect. Therefore, it is an attractive strategy to study an effective and safe oral vaccine delivery system that can promote gastrointestinal mucosal immune responses and inhibit antigen degradation. Moreover, the antigens uptake by microfold cells (M cel...
