Gallo, Giuseppe Schillaci, Domenico
Published in
Applied microbiology and biotechnology
The widespread use of antibiotics has resulted in the outbreak and spread of antibiotic-resistant pathogens. Bacterial antibiotic resistance may develop at cellular and community levels. In the latter case, it is based on tolerance which implicates the shift from a free-living form of life (i.e., planktonic) to a sessile multi-stratified community ...
Urlacher, Vlada B Koschorreck, Katja
Published in
Applied microbiology and biotechnology
Aryl-alcohol oxidases (AAOs) are FAD-containing enzymes that oxidize a broad range of aromatic as well as aliphatic allylic alcohols to aldehydes. Their broad substrate spectrum accompanied by the only need for molecular oxygen as cosubstrate and production of hydrogen peroxide as sole by-product makes these enzymes very promising biocatalysts. AAO...
Shleeva, Margarita Savitsky, Alexander Kaprelyants, Arseny
Published in
Applied microbiology and biotechnology
The spread of multi-drug-resistant bacterial strains causing serious infectious diseases dictates the development of new approaches to combat these diseases. In addition to drug resistance, the important causative agent of tuberculosis (Mycobacterium tuberculosis (Mtb)) is able to persist asymptomatically in individuals for many years, causing late...
Klausmann, Peter Hennemann, Katja Hoffmann, Mareen Treinen, Chantal Aschern, Moritz Lilge, Lars Morabbi Heravi, Kambiz Henkel, Marius Hausmann, Rudolf
Published in
Applied microbiology and biotechnology
Bacillus subtilis 3NA is a strain capable of reaching high cell densities. A surfactin producing sfp+ variant of this strain, named JABs32, was utilized in fed-batch cultivation processes. Both a glucose and an ammonia solution were fed to set a steady growth rate μ of 0.1 h-1. In this process, a cell dry weight of up to 88 g L-1 was reached after ...
Myszka, Kamila Tomaś, Natalia Wolko, Łukasz Szwengiel, Artur Grygier, Anna Nuc, Katarzyna Majcher, Małgorzata
Published in
Applied microbiology and biotechnology
The present study aimed to elucidate the effect of subinhibitory concentrations (sub-MICs) of juniper essential oil (EO), α-pinene, and sabinene on the quorum-sensing (QS)-mediated proteolytic and lipolytic properties of Pseudomonas fluorescens KM24. These activities were verified under in situ conditions, in which sub-MICs of the agents altered th...
Becker, Aileen Böttcher, Dominique Katzer, Werner Siems, Karsten Müller-Kuhrt, Lutz Bornscheuer, Uwe T
Published in
Applied microbiology and biotechnology
Raspberry ketone is a widely used flavor compound in food and cosmetic industry. Several processes for its biocatalytic production have already been described, but either with the use of genetically modified organisms (GMOs) or incomplete conversion of the variety of precursors that are available in nature. Such natural precursors are rhododendrol ...
Keysberg, Christoph Hertel, Oliver Schelletter, Louise Busche, Tobias Sochart, Chiara Kalinowski, Jörn Hoffrogge, Raimund Otte, Kerstin Noll, Thomas
Published in
Applied microbiology and biotechnology
In biopharmaceutical production, Chinese hamster ovary (CHO) cells derived from Cricetulus griseus remain the most commonly used host cell for recombinant protein production, especially antibodies. Over the last decade, in-depth multi-omics characterization of these CHO cells provided data for extensive cell line engineering and corresponding incre...
Vieira, Antonio Diogo Silva de Souza, Carlota Bussolo Padilha, Marina Zoetendal, Erwin Gerard Smidt, Hauke Saad, Susana Marta Isay Venema, Koen
Published in
Applied microbiology and biotechnology
The aim of this study was to evaluate the effects of soy-based beverages manufactured with water-soluble soy extract, containing probiotic strains (Lactobacillus acidophilus LA-5 and Bifidobacterium longum BB-46) and/or acerola by-product (ABP) on pooled faecal microbiota obtained from lean and obese donors. Four fermented soy beverages (FSs) ("pla...
Li, Jingqi Liu, Sixu Gao, Yanlin Tian, Shuaishuai Yang, Yu Ma, Ningning
Published in
Applied microbiology and biotechnology
N-linked glycosylation plays critical roles in folding, receptor binding, and immunomodulating of hemagglutinin (HA), the main antigen in influenza vaccines. Chicken embryos are the predominant production host for influenza vaccines, but Madin-Darby canine kidney (MDCK) cells have emerged as an important alternative host. In this study, we compared...
Liu, Zhiyong Zhang, Rongzhen Zhang, Wenchi Xu, Yan
Published in
Applied microbiology and biotechnology
NAD(H)-dependent 7α-hydroxysteroid dehydrogenase catalyzes the oxidation of chenodeoxycholic acid to 7-oxolithocholic acid. Here, we designed mutations of Ile258 adjacent to the catalytic pocket of Brucella melitensis 7α-hydroxysteroid dehydrogenase. The I258M variant gave a 4.7-fold higher kcat, but 4.5-fold lower KM, compared with the wild type, ...
