Kim, Yeon-Ji Kim, Min-Jung Kweon, Dong-Keon Lim, Seung-Taik Lee, Sung-Joon
Published in
Applied biochemistry and biotechnology
The regulation of mitochondrial biogenesis, melanogenesis, and connective tissue proteins is critical for homeostasis and aging skin cells. We examined the biological effects of polydeoxyribonucleotide (PDRN) on mitochondrial biogenesis, melanogenesis, and connective tissue proteins in vitro. In a radical scavenging assay, PDRN showed antioxidant a...
Zhang, Xiujuan Li, Wenjuan Dou, Xiang Nan, De He, Gaohong
Published in
Applied biochemistry and biotechnology
Astaxanthin (AST) has attracted great interests in the scientific world because of its anti-oxidative, anti-inflammatory properties. And biodegradable materials, like chitosan, have been employed as the AST carrier to protect it from degradation and promote its bioavailability. However, the lack of pH responsiveness of these materials usually could...
Teksoy, Ozgun Sahinturk, Varol Cengiz, Mustafa İnal, Behcet Ayhancı, Adnan
Published in
Applied biochemistry and biotechnology
This study aims to investigate the protective effects of silymarin (Sm) in thioacetamide (TAA)-related liver damage. What makes this study special is that it attempts to determine the expression of changes in the liver at the level of gene expression. Routine liver damage markers were compared with changes in the levels of microRNA (miRNA) known as...
Bidja Abena, Marie Thérèse Sodbaatar, Naranjargal Li, Tongtong Damdinsuren, Narantuya Choidash, Battsetseg Zhong, Weihong
Published in
Applied biochemistry and biotechnology
Bioremediation has been attracting researchers' attention to develop as a technique to remove the pollution of crude oil in the environment. However, more or stronger novel strains capable of crude oil removal are still required. In this study, the potential of five newly isolated bacterial strains for crude oil abatement was evaluated in the liqui...
Padilha, G S Osório, W R
Published in
Applied biochemistry and biotechnology
In order to recover biomolecules, a single downstream processing step is carried out. In this sense, an aqueous two-phase system (ATPS) containing polyethylene glycol (PEG) and potassium phosphate salts is used. Intending the purification of Burkholderia cepacia (Bc) lipase, the effects of the molecular masses of 1500 (PEG 1500), 4000 (PEG 4000), a...
Gedam, Preety S Raut, Atulkumar N Dhamole, Pradip B
Published in
Applied biochemistry and biotechnology
The foremost aim of the present study was to enhance butanol production in an extractive fermentation study in presence of non-ionic surfactant using immobilized cells. Earlier studies had shown improved butanol production with non-ionic surfactant and immobilized cells independently. Therefore, in the present work, the combined effect of extractiv...
Khezri, Jafar Yakhchali, Bagher Karkhane, Ali Asghar Sangtarash, Mohammad Hossein
Published in
Applied biochemistry and biotechnology
Site-directed mutagenesis is one of the most important tools in molecular biology. The majority of the mutagenesis methods have been developed to mutate one region of target DNA in each cycle of mutagenesis, while in some cases there is a need to mutate several distal points. We used a new method to simultaneously mutate two distal points in the ta...
Hu, Song Zhao, Xiao Zhang, Li
Published in
Applied biochemistry and biotechnology
As a type of β-N-acetyl-D-hexosaminidase enzyme purified from the Ostriniafurnacalis (Asian corn borer), OfHex1 has been previously reported to participate in chitin degradation, indicating that it may be an ideal target for designing new environmentally friendly pesticides. Besides, a natural product, TMG-chitotriomycin, has been found to be an ef...
Meng, Zhen Yang, Qin-Zheng Wang, Jing-Zhen Hou, Yun-Hua
Published in
Applied biochemistry and biotechnology
Feruloyl esterases (FAEs) have great potential applications in paper and breeding industry. A new thermo-stable feruloyl esterase gene, TtfaeB was identified from the thermophilic fungus Thielavia terrestris h408. Deduced protein sequence shares the identity of 67% with FAEB from Neurospora crassa. The expression vector pPIC9K-TtfaeB was successful...
Hamid, Attia Aftab, Muhammad Nauman
Published in
Applied biochemistry and biotechnology
The gene of a β-xylanase (Tnap_0700) was cloned from a hyperthermophilic Thermotoga naphthophila strain ATCC BAA-489 and expressed in Escherichia coli BL21 (DE3) via pET-21a (+) as an expression vector. The growth steps were upgraded for highest β-xylanase expression via several factors, for example, IPTG concentration, time of induction, pH, and t...
