Xiong, Yifan Cao, Gaihua Chen, Xiaolong Yang, Jun Shi, Meimei Wang, Yu Nie, Fuping Huo, Danqun Hou, Changjun
Published in
Applied microbiology and biotechnology
The livestock industry has been deeply affected by African swine fever virus (ASFV) and Capripoxvirus (CaPV), which caused an enormous economic damage. It is emergent to develop a reliable detection method. Here, we developed a rapid, ultra-sensitive, and one-pot DNA detection method combining recombinase polymerase amplification (RPA) and CRISPR/C...
Wang, Meng Deng, Zifeng Li, Yanmei Ma, Yi Wang, Jufang
Published in
Applied Microbiology and Biotechnology
Abstract Clostridium difficile ( C. difficile ) is a Gram-positive, spore-forming, toxin-producing anaerobe that can cause nosocomial antibiotic-associated intestinal disease. Autolysin is a lytic enzyme that hydrolyzes peptidoglycans of the bacterial cell wall, with a catalytic domain and cell wall–binding domains, proven to be involved in bacteri...
Checcucci, A. Luise, D. Modesto, M. Correa, F. Bosi, P. Mattarelli, P. Trevisi, Paolo
Published in
Applied Microbiology and Biotechnology
Moghadam, Majid Taati Mojtahedi, Ali Moghaddam, Mehrdad Moosazadeh Fasihi-Ramandi, Mahdi Mirnejad, Reza
Published in
Applied microbiology and biotechnology
It has been about a century since the discovery of the first antibiotic, and during this period, several antibiotics were produced and marketed. The production of high-potency antibiotics against infections led to victories, but these victories were temporary. Overuse and misuse of antibiotics have continued to the point that humanity today is almo...
Urbański, Linda J. Bua, Silvia Angeli, Andrea Emameh, Reza Zolfaghari Barker, Harlan R. Kuuslahti, Marianne Hytönen, Vesa P. Parkkila, Seppo Supuran, Claudiu T.
Published in
Applied Microbiology and Biotechnology
Abstract We report the production and biochemical characterization of an α-carbonic anhydrase (LrhCA) from gram-positive probiotic bacteria Lactobacillus rhamnosus GG. CAs form a family of metalloenzymes that catalyze hydration of CO2/interconversion between CO2 and water to bicarbonate ions and protons. They are divided into eight independent gene...
Zhao, Huijun Ren, Jiahui Wu, Shuya Guo, Haoran Du, Yongkun Wan, Bo Ji, Pengchao Wu, Yanan Zhuang, Guoqing Zhang, Angke
...
Published in
Applied Microbiology and Biotechnology
Abstract African swine fever (ASF), which is caused by the ASF virus (ASFV), is a highly contagious hemorrhagic disease that causes high mortality to domestic porcine and wild boars and brings huge economic losses to world swine industry. Due to the lack of an effective vaccine, the control of ASF must depend on early, efficient, and cost-effective...
Kibria, K. M. Kaderi Faruque, Md. Omar Islam, Md. Shaid bin Ullah, Hedayet Mahmud, Shafi Miah, Mojnu Saleh, Amani Ali
Published in
Applied Microbiology and Biotechnology
Abstract Novel coronavirus (SARS-CoV-2) leads to coronavirus disease 19 (COVID-19), declared as a pandemic that outbreaks within almost 225 countries worldwide. For the time being, numerous mutations have been reported that led to the generation of numerous variants spread more rapidly. This study aims to establish an efficient multi-epitope subuni...
Barolo, Lorenzo Commault, Audrey S. Abbriano, Raffaela M. Padula, Matthew P. Kim, Mikael Kuzhiumparambil, Unnikrishnan Ralph, Peter J. Pernice, Mathieu
Published in
Applied Microbiology and Biotechnology
Abstract The green microalga Chlamydomonas reinhardtii is emerging as a promising cell biofactory for secreted recombinant protein (RP) production. In recent years, the generation of the broadly used cell wall–deficient mutant strain UVM4 has allowed for a drastic increase in secreted RP yields. However, purification of secreted RPs from the extrac...
Pócsi, István Szigeti, Zsuzsa M. Emri, Tamás Boczonádi, Imre Vereb, György Szöllősi, János
Published in
Applied Microbiology and Biotechnology
Abstract While phototoxicity can be a useful therapeutic modality not only for eliminating malignant cells but also in treating fungal infections, mycologists aiming to observe morphological changes or molecular events in fungi, especially when long observation periods or high light fluxes are warranted, encounter problems owed to altered regulator...
Pavlović, Jelena Bosch-Roig, Pilar Rusková, Magdalena Planý, Matej Pangallo, Domenico Sanmartín, Patricia
Published in
Applied Microbiology and Biotechnology
Abstract The irregular damp dark staining on the stonework of a salt-contaminated twelfth century granite-built chapel is thought to be related to a non-homogeneous distribution of salts and microbial communities. To enhance understanding of the role of microorganisms in the presence of salt and damp stains, we determined the salt content and ident...
