Kota, Rohini Krishna Kolla, Harish Babu Reddy, Prakash Narayana Kalagatur, Naveen Kumar Samudrala, Siva Kumar
Published in
Applied Microbiology and Biotechnology
Abstract Staphylococcus aureus is a serious pathogen unleashing its virulence through several classes of exotoxins such as hemolysins and enterotoxins. In this study, we designed a novel multi-antigen subunit vaccine which can induce innate, humoral and cellular immune responses. Alpha hemolysin, enterotoxins A and B were selected as protective ant...
Zakataeva, Natalia P.
Published in
Applied Microbiology and Biotechnology
5′-Nucleotidases (EC 3.1.3.5) are enzymes that catalyze the hydrolytic dephosphorylation of 5′-ribonucleotides and 5′-deoxyribonucleotides to their respective nucleosides and phosphate. Most 5′-nucleotidases have broad substrate specificity and are multifunctional enzymes capable of cleaving phosphorus from not only mononucleotide phosphate molecul...
Bajerski, Felizitas Nagel, Manuela Overmann, Joerg
Published in
Applied Microbiology and Biotechnology
Abstract Modern biobanks maintain valuable living materials for medical diagnostics, reproduction medicine, and conservation purposes. To guarantee high quality during long-term storage and to avoid metabolic activities, cryostorage is often conducted in the N2 vapour phase or in liquid nitrogen (LN) at temperatures below − 150 °C. One potential ri...
Gupta, Rahul Kazi, Tawsif Ahmed Dey, Dhritiman Ghosh, Arijit Ravichandiran, V. Swarnakar, Snehasikta Roy, Syamal Biswas, Swadesh Ranjan Ghosh, Dipanjan
Published in
Applied Microbiology and Biotechnology
Abstract The emergence of SARS-CoV-2 has brought the world to a standstill, and till date, effective treatments and diagnostics against this idiosyncratic pathogen are lacking. As compared to the standard WHO/CDC qPCR detection method, which consumes several hours for detection, CRISPR-based SHERLOCK, DETECTR, and FELUDA have emerged as rapid diagn...
Hein, Marc D. Chawla, Anshika Cattaneo, Maurizio Kupke, Sascha Y. Genzel, Yvonne Reichl, Udo
Published in
Applied Microbiology and Biotechnology
Respiratory diseases including influenza A virus (IAV) infections represent a major threat to human health. While the development of a vaccine requires a lot of time, a fast countermeasure could be the use of defective interfering particles (DIPs) for antiviral therapy. IAV DIPs are usually characterized by a large internal deletion in one viral RN...
Liu, Xi Zhou, Zhong-Ya Cui, Jin-Long Wang, Meng-Liang Wang, Jun-Hong
Published in
Applied Microbiology and Biotechnology
Abstract Increased understanding of the interactions between endophytic fungi and plants has led to the discovery of a new generation of chemical compounds and processes between endophytic fungi and plants. Due to the long-term co-evolution between fungal endophytes and host plants, endophytes have evolved special biotransformation abilities, which...
Rosic, Nedeljka N.
Published in
Applied Microbiology and Biotechnology
Abstract Bioactive compounds from marine environments represent a rich source of bioproducts for potential use in medicine and biotechnology. To discover and identify novel marine natural products (MNPs), evaluating diverse biological activities is critical. Increased sensitivity and specificity of omics technologies, especially next-generation hig...
Wawrosch, Christoph Zotchev, Sergey B.
Published in
Applied Microbiology and Biotechnology
Medicinal plants have been used by mankind since ancient times, and many bioactive plant secondary metabolites are applied nowadays both directly as drugs, and as raw materials for semi-synthetic modifications. However, the structural complexity often thwarts cost-efficient chemical synthesis, and the usually low content in the native plant necessi...
Fessner, Nico D. Nelson, David R. Glieder, Anton
Published in
Applied Microbiology and Biotechnology
Abstract Bioprospecting for innovative basidiomycete cytochrome P450 enzymes (P450s) is highly desirable due to the fungi’s enormous enzymatic repertoire and outstanding ability to degrade lignin and detoxify various xenobiotics. While fungal metagenomics is progressing rapidly, the biocatalytic potential of the majority of these annotated P450 seq...
Isogai, Shota Takagi, Hiroshi
Published in
Applied Microbiology and Biotechnology
Abstract Lysine, a nutritionally important amino acid, is involved in adaptation and tolerance to environmental stresses in various organisms. Previous studies reported that lysine accumulation occurs in response to stress and that lysine supplementation enhances stress tolerance; however, the effect of lysine biosynthesis enhancement on stress tol...
