Lopes, Ana Azevedo-Silva, João Carsanba, Erdem Pintado, Manuela Oliveira, Ana Sofia Ferreira, Carlos Pereira, Joana Odila Carvalho, Ana P Oliveira, Carla
Published in
Applied microbiology and biotechnology
Yeast cells face various stress factors during industrial fermentations, since they are exposed to harsh environmental conditions, which may impair biomolecules productivity and yield. In this work, the use of an antioxidant peptide extract obtained from industrial spent yeast was explored as supplement for Saccharomyces cerevisiae fermentation to ...
Fang, Jianhua Liu, Jing Cheng, Na Kang, Xiuhua Huang, Zhanchao Wang, Guoyu Xiong, Xiaofeng Lu, Tian Gong, Zhenghua Huang, Zhigang
...
Published in
Applied Microbiology and Biotechnology
Abstract The outbreak of coronavirus disease 2019 (COVID-19) in 2019 has severely damaged the world's economy and public health and made people pay more attention to respiratory infectious diseases. However, traditional quantitative real-time polymerase chain reaction (qRT-PCR) nucleic acid detection kits require RNA extraction, reverse transcripti...
Jing, Zhanyu Xu, Jian Liu, Jia Du, Cong Qi, Jiakun Fan, Chao Li, Yimin Yuan, Wenjie
Published in
Applied Microbiology and Biotechnology
Abstract L-Alanyl-L-Glutamine (Ala-Gln) is a common parenteral nutritional supplement. In our previous study, the recombinant whole-cell catalyst Escherichia coli BL21(DE3) overexpressing α-amino acid ester acyltransferase (BPA) to produce Ala-Gln has high activity and has been applied to large-scale production experiments. However, the degradation...
Vizzini, Priya Beltrame, Elena Coppedè, Nicola Vurro, Filippo Andreatta, Francesco Torelli, Emanuela Manzano, Marisa
Published in
Applied Microbiology and Biotechnology
Abstract Foods contaminated by pathogens are responsible for foodborne diseases which have socioeconomic impacts. Many approaches have been extensively investigated to obtain specific and sensitive methods to detect pathogens in food, but they are often not easy to perform and require trained personnel. This work aims to propose a textile organic e...
Oliveira, Hugo Santos, Sílvio Pires, Diana P. Boeckaerts, Dimitri Pinto, Graça Domingues, Rita Otero, Jennifer Briers, Yves Lavigne, Rob Schmelcher, Mathias
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Published in
Applied Microbiology and Biotechnology
Abstract Citrobacter koseri is an emerging Gram-negative bacterial pathogen, which causes urinary tract infections. We isolated and characterized a novel S16-like myovirus CKP1 (vB_CkoM_CkP1), infecting C. koseri . CkP1 has a host range covering the whole C. koseri species, i.e. , all strains that were tested, but does not infect other species. Its...
Bimmer, Martin Reimer, Martin Klingl, Andreas Ludwig, Christina Zollfrank, Cordt Liebl, Wolfgang Ehrenreich, Armin
Published in
Applied microbiology and biotechnology
Bacterial cellulose (BC) represents a renewable biomaterial with unique properties promising for biotechnology and biomedicine. Komagataeibacter hansenii ATCC 53,582 is a well-characterized high-yield producer of BC used in the industry. Its genome encodes three distinct cellulose synthases (CS), bcsAB1, bcsAB2, and bcsAB3, which together with gene...
Li, Chia-Jung Jiang, Chung-Lin Chao, Tai-Ling Lin, Shiau-Yu Tsai, Ya-Min Chao, Chong-Syun Su, Yu-Ting Chen, Chun-Jen Chang, Sui-Yuan Lin, Fu-Jung
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Published in
Applied microbiology and biotechnology
Vaccination is considered to be the most effective countermeasure to prevent and combat the global health threats of COVID-19. People with obesity are at a greater risk of hospitalization, life-threatening illness, and adverse outcomes after having COVID-19. Therefore, a safe and effective COVID-19 vaccine for obese individuals is urgently needed. ...
Müller, Markus Kües, Ursula Budde, Katharina B Gailing, Oliver
Published in
Applied microbiology and biotechnology
Forests provide invaluable economic, ecological, and social services. At the same time, they are exposed to several threats, such as fragmentation, changing climatic conditions, or increasingly destructive pests and pathogens. Trees, the inherent species of forests, cannot be viewed as isolated organisms. Manifold (micro)organisms are associated wi...
Garay, Ernesto Fontana, Diego Villarraza, Javier Fuselli, Antonela Gugliotta, Agustina Antuña, Sebastián Tardivo, Belén Rodríguez, María Celeste Gastaldi, Victoria Battagliotti, Juan Manuel
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Published in
Applied Microbiology and Biotechnology
Abstract Due to the high number of doses required to achieve adequate coverage in the context of COVID-19 pandemics, there is a great need for novel vaccine developments. In this field, there have been research approaches that focused on the production of SARS-CoV-2 virus-like particles. These are promising vaccine candidates as their structure is ...
Villarraza, Javier Fuselli, Antonela Gugliotta, Agustina Garay, Ernesto Rodríguez, María Celeste Fontana, Diego Antuña, Sebastián Gastaldi, Victoria Battagliotti, Juan Manuel Tardivo, María Belén
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Published in
Applied microbiology and biotechnology
Spike protein from SARS-CoV-2, the etiologic agent of the COVID-19 pandemic disease, constitutes a structural protein that proved to be the main responsible for neutralizing antibody production. Thus, its sequence is highly considered for the design of candidate vaccines. Animal cell culture represents the best option for the production of subunit ...
