Argentova, V. V. Aliev, T. K. Gasparyan, M. E. Dolgikh, D. A. Kirpichnikov, M. P.
Published in
Applied Biochemistry and Microbiology
Several serum-free media have been developed and are successfully used at present for the cultivation of various recombinant eukaryotic cell lines. Therapeutic recombinant IgA antibodies have recently been increasingly used along with numerous IgG1 isotype antibodies. Therefore, it became necessary to improve the growth characteristics, metabolism,...
Yurkova, M. S. Zenin, V. A. Sadykhov, E. G. Fedorov, A. N.
Published in
Applied Biochemistry and Microbiology
The study of antimicrobial peptides (AMPs), which are considered a source of new antibiotics, have recently attracted more and more attention. AMPs combine several large groups of peptides with different spectra and different mechanisms of action on bacterial cells. Peptides that form alpha-helices are better studied; the mechanisms of antimicrobia...
Bubnov, D. M. Yuzbashev, T. V. Fedorov, A. S. Bondarenko, F. V. Savchenko, A. S. Vybornaya, T. V. Filippova, S. S. Sineoky, S. P.
Published in
Applied Biochemistry and Microbiology
AbstractThe present work describes an approach that uses a reduction in biomass accumulation during fermentation to improve the properties of a strain producing L-threonine. Glutamyl- and glutaminyl-tRNA synthetases were chosen as targets. Mutants carrying temperature-sensitive alleles of the mentioned enzymes were obtained. It was shown with this ...
Vybornaya, T. V. Yuzbashev, T. V. Fedorov, A. S. Bubnov, D. M. Filippova, S. S. Bondarenko, F. V. Sineoky, S. P.
Published in
Applied Biochemistry and Microbiology
AbstractAn E. coli strain in which all known pathways of threonine catabolism were inactivated (Δtdh, ΔltaE, ΔilvA, ΔtdcB, ΔyiaY) has been constructed. It was shown that the expression of heterologous citramalate synthase from Leptospira interrogans in an E. coli strain carrying the ΔilvA deletion can serve as an alternative pathway for isoleucine ...
Ryabchenko, L. E. Leonova, T. E. Shustikova, T. E. Gerasimova, T. V. Ivankova, T. A. Sidorenko, K. V. Yanenko, A. S.
Published in
Applied Biochemistry and Microbiology
AbstractThe psefdh_D221Q gene encodes a mutant formate dehydrogenase from Pseudomonas (P-seFDG_D221Q), which catalyzes formate oxidation with the simultaneous formation of NADPH. It is expressed in cells of lysine-producing Corynebacterium glutamicum strains. The psefdh_D221Q gene was introduced into C. glutamicum strains as part of an autonomous p...
Gulevich, A. Yu. Skorokhodova, A. Yu. Debabov, V. G.
Published in
Applied Biochemistry and Microbiology
AbstractThe possible contribution of collateral enzymes to the formation of the key precursor metabolite, 3-hydroxybutyryl-CoA, has been evaluated in a recombinant Escherichia coli strain engineered for 1,3-butanediol biosynthesis from glucose via the inverted fatty-acid beta-oxidation pathway. Inactivation of the 3-hydroxyadipyl-CoA dehydrogenase ...
Begunova, A. V. Rozhkova, I. V. Shirshova, T. I. Glazunova, O. A. Fedorova, T. V.
Published in
Applied Biochemistry and Microbiology
The article presents the results of optimization of the culturing conditions to increase the production of bacteriocin-like compounds (presumably including reuterin) by the Lactobacillus reuteri strain LR1. The strain Escherichia coli ATCC 25922, an opportunistic microorganism, the content of which is standardized in foods by the Food Safety Legisl...
Raigorodskaya, M. P. Turchinovich, A. Tsypina, I. M. Zgoda, V. G. Nikulin, S. V. Maltseva, D. V.
Published in
Applied Biochemistry and Microbiology
AbstractThe cytotoxic effect of 5-fluorouracil (5FU) and regorafenib (RF), drugs with different mechanisms of action used to treat colorectal cancer, on an HT29 cell line cultured on plastic or laminin 521 (LM-521) has been studied. It is first shown that LM-521 can increase the sensitivity of tumor cells to 5FU. A possible mechanism of the observe...
Vdovina, T. V. Sirotkin, A. S. Kobeleva, Y. V. Gorshkova, E. S.
Published in
Applied Biochemistry and Microbiology
The efficiency of the bioaugmentation of nitrifying bacteria into biofilm microbiocenosis with 30 days of continuous biofiltration of a solution of municipal model wastewater has been assessed. The laboratory setup consisted of two parallel operating biofilters. Cultures of ammonium-oxidizing and nitrite-oxidizing bacteria of the Nitrobacter genus ...
Gordeeva, T. L. Borshchevskaya, L. N. Kalinina, A. N. Bulushova, N. V. Syneoky, S. P. Voronin, S. P. Kashirskaya, M. D.
Published in
Applied Biochemistry and Microbiology
AbstractA DNA sequence from Kosakonia sacchari that, according to automated computer analysis, is believed to correspond to the gene for histidine-acid phytase has been selected from the GenBank database. The sequence was optimized for codon composition, synthesized, cloned, and expressed in Pichia pastoris. The main characteristics of the purified...
