Hou, Y. Lu, F. Tian, J. Tian, Y.
Published in
Applied Biochemistry and Microbiology
AbstractAn intracellular serine protease designated ISPr from newly isolated salt-tolerant Bacillus sp. LCB10 was expressed in Escherichia coli. The isp gene was cloned into pET30a vector and expressed in E. coli BL21 (DE3). After purification, the molecular mass of ISPr was estimated to be 35 kDa by SDS-PAGE and the specific activity was 384 U/mg....
Shanin, I. A. Zvereva, E. A. Eremin, S. A. Sviridov, O. V. Zherdev, A. V. Dzantiev, B. B.
Published in
Applied Biochemistry and Microbiology
AbstractA method for preparing antibodies that specifically recognize antibiotics of the fluoroquinolone group with the same radical in the first position of the quinolone nucleus is proposed. The specificity of rabbit antisera prepared at different cycles of immunization by changing the structure of the hapten in the composition of immunogens was ...
Doynikova, A. N. Vekshin, N. L.
Published in
Applied Biochemistry and Microbiology
AbstractFluorescence spectroscopy was used to study the specific hydrolysis of ribosomal RNA by binase in aqueous buffer solutions. Acridine orange was used for RNA staining. When the dye was bound to RNA, its fluorescence intensity increased by two times due to the formation of a stable complex. During RNA hydrolysis by binase for 1–20 min, this c...
Plyushchenko, A. V. Borovikova, L. N. Pisarev, O. A.
Published in
Applied Biochemistry and Microbiology
AbstractTwo methods for the synthesis of hybrid organic-inorganic nanocomplexes comprising chymotrypsin and silver nanoparticles have been developed based on the chemical reduction of silver nitrate by sodium borohydride. In the first method, the enzyme is added to the reaction mixture simultaneously with other reagents. In the second method, it is...
Serdyuk, E. G. Isakova, E. P. Gessler, N. N. Trubnikova, E. V. Antipov, A. N. Deryabina, Y. I.
Published in
Applied Biochemistry and Microbiology
AbstractThe characteristics of recombinant phytase from Obesumbacterium proteus have been studied after its integration into Yarrowia lipolytica with a Po1f (pUV3-Op) plasmid. Phytase activity was observed in Y. lipolytica Po1f transformants in a wide pH range (3.5–7.5) and remained at 80% of the initial value after 3-min heating at 95°C, which mad...
Poshekhontseva, V. Yu. Fokina, V. V. Sukhodolskaya, G. V. Shutov, A. A. Donova, M. V.
Published in
Applied Biochemistry and Microbiology
AbstractThe dependence of biosynthesis of macrolide immunosuppressive agent tacrolimus (FK-506) in starch-containing media by the Streptomyces tsukubaensis VKM Ac-2618D strain on the molecular weight and composition of potato starch polysaccharides was studied. It was shown that the use of high-molecular branched starches as a carbon source provide...
Theansungnoen, T. Jangpromma, N. Anwised, P. Daduang, S. Fukumori, Y. Taoka, A. Klaynongsruang, S.
Published in
Applied Biochemistry and Microbiology
AbstractKT2 is a cationic antimicrobial peptide belonging to Crocodylus siamensis leucrocin I analogs. The mode of action of this compound taken at lethal concentration includes translocation into bacterial cells where binding to DNA is presumed to occur. However, the effects of KT2 on bacterial membrane have not been completely elucidated to date....
Guzmán-Martín, J. L. González-Bustos, P. Gutiérrez-Fernández, J.
Published in
Applied Biochemistry and Microbiology
AbstractCampylobacter spp. are the principal bacteria causing human gastroenteritis globally. Recently, several species of Campylobacter other than C. jejuni and C. coli have been identified. These emerging species have been associated with undiagnosed intestinal infectious diseases, although their clinical importance has not been determined yet. I...
Qiao, M.-F. Wu, H-C. Liu, Y. Lu, Y. Deng, J.
Published in
Applied Biochemistry and Microbiology
AbstractAn aroma-producing strain B15 was isolated from a spontaneous fermentation culture of traditional Chinese sweet bean sauce (Tian Wei Food Co., Ltd., China), and identified as Bacillus subtilis, exhibiting an ability to synthesize acetoin. In order to better understand the influence of salt stress on acetoin metabolism, a metabolic network o...
Eprintsev, A. T. Komarova, N. R. Falaleeva, M. I. Beloglazova, A. A.
Published in
Applied Biochemistry and Microbiology
AbstractThe placement of pea plants (Pisum sativum L.) under flooding conditions led to an increase in lactate dehydrogenase (EC 1.1.1.27) activity in the roots. The enzyme was purified to an electrophoretic homogeneous state by a multistage purification method including ammonium sulfate fractionation, ion exchange chromatography on DEAE-Sephacel, ...
