Published in Applied biochemistry and microbiology
The global probiotics industry has been undergoing major changes in recent years. Approaches to finding and creating new probiotics, as well as a paradigm of their use in food, medicine, and pharmacology are changing. The catalyst proved to be the increasing popularity and availability of omics technologies, in particular, metagenomic studies of hu...
Published in Applied biochemistry and microbiology
A biologically active composition of chitosan-selenium nanoparticles has been developed. Selenium nanoparticles are characterized by a clear bimodal size distribution: 2-3 and ~37 nm. The main active centers of complexation with nanoparticles are the amino and hydroxyl groups of chitosan. In experiments on culturing fibroblasts of the hTERT BJ-5ta ...
Published in Applied Biochemistry and Microbiology
AbstractCarbamates are among the most commonly used xenobiotics as insecticides, even though these compounds are highly toxic to humans and animals. Within carbamates, bendiocarb has been used against a vast range of nuisance and disease vector insects and in agriculture on sugar beet and maize crops. Since 1999, the Environmental Protection Agency...
Published in Applied Biochemistry and Microbiology
AbstractThe drug resistance pattern of infectious A. baumannii isolates of Saudi Arabia was evaluated and the integrons in samples were characterized. The isolates were obtained from patients of King Abdulaziz University Hospital, Jeddah, Saudi Arabia. The aim of this study was to investigate the association between drug resistance and the presence...
Published in Applied Biochemistry and Microbiology
AbstractBacillus velezensis is regarded as a potential starter culture for food, due to its biological safety and efficient protein hydrolysis ability. To clarify the diversity of extracellular proteases produced by B. velezensis SW5 isolated from fish sauce, substrate immersing zymography and peptide analysis based on MS were used. A total of 12 p...
Published in Applied Biochemistry and Microbiology
AbstractEndophytic fungi isolated from the insectivorous plant Drosera burmannii were tested for their antioxidant potential. The isolate Dro2 was found to have superlative antioxidant activity of the culture broth, scavenging 57.6 ± 0.2% of 2,2-diphenyl-1-picrylhydrazyl free radical. 5.8S rDNA homology led to identification of the isolate as a new...
Published in Applied Biochemistry and Microbiology
AbstractNeurotrophic factors, including neurturin, increase the life span of the neurons and prevent neuronal destruction. As recombinant expression of neurturin with 3 disulfide bonds in Escherichia coli leads to the formation of inclusion bodies in the cytoplasm, periplasmic production of it is preferred. Signal peptides naturally exist at the N-...
Published in Applied Biochemistry and Microbiology
Naphthoquinones harboring 1,4-naphthoquinone pharmacophore are considered as privileged structures in medicinal chemistry. In pharmaceutical industry and fundamental research, polyketide naphthoquinones were widely produced by heterologous expression of polyketide synthases in microbial chassis cells, such as Saccharomyces cerevisiae and Escherichi...
Published in Applied Biochemistry and Microbiology
AbstractThe thermostable xylanase from Aspergillus fumigatus JCM 10253 was purified and characterized. The purification of the enzyme was carried out by 80% ammonium sulphate precipitation followed by dialysis and DEAE-cellulose ion-exchange chromatography with a 17.1% yield. The molecular weight of purified xylanase was approximately 19 kDa. The o...
Published in Applied Biochemistry and Microbiology
AbstractWith the development of metabolic engineering and synthetic biology, a large number of industrially important products has been developed using engineered microorganisms, thereby promoting the sustainable development of society and the economy. However, this approach is challenged by the increasing demand for more complicated biosynthesis o...
