Ueyama, Jun Oda, Masaya Hirayama, Masaaki Sugitate, Kuniyo Sakui, Norihiro Hamada, Risa Ito, Mikako Saito, Isao Ohno, Kinji
Published in
Analytical biochemistry
The analysis methods for fecal short-chain fatty acids (SCFAs) have evolved considerably. Recently, the role of SCFAs in gastrointestinal physiology and their association with intestinal microbiota and disease were reported. However, the intra-fecal variability and storage stability of SCFAs have not been extensively investigated. The aim of this s...
Huang, Dezhi Ying, Haojun Jiang, Dongneng Liu, Fei Tian, Ye Du, Chunlan Zhang, Liqun Pu, Xiaoyun
Published in
Analytical biochemistry
Interleukin 6 (IL-6) is an interleukin that acts as both a proinflammatory and anti-inflammatory cytokine. It can be used as a potential diagnostic biomarker for sepsis. The aim of this study was to establish an easy-to-use detection kit for rapid, quantitative and on-site detection of IL-6. To develop the new IL-6 quantitative detecting kit, a dou...
Deng, Tingting Huang, Wensheng Ren, Junan Ma, Xiuli Ge, Yiqiang Chen, Ying
Published in
Analytical biochemistry
To standardize the rice-specific quantification methods, the criteria of six genes of rice (gos9, PLD, SPS, RBE4, ppi-PPF and oriazain) were compared and evaluated by ddPCR. The results revealed that SPS, RBE4 and ppi-PPF were single copy genes per haploid genome and species specificity and stable among different rice cultivars, by employing Lectin...
Asano, Yasuhisa
Published in
Analytical biochemistry
L, Potter D, Krusienski J, Kennedy Cl, Hoppel N, Lai
Published in
Analytical biochemistry
Bioenergetic function is characterized with assays obtained by polarographic systems. Analog systems without data acquisition, visualization, and processing tools are used but require cumbersome operations to derive respiration rate and ADP to oxygen stoichiometry of oxidative phosphorylation (ADP/O ratio). The analog signal of a polarograhic syste...
Bauer, Tyler M Giles, Abigail V Sun, Junhui Femnou, Armel Covian, Raul Murphy, Elizabeth Balaban, Robert S
Published in
Analytical biochemistry
Tissue transmission optical absorption spectroscopy provides dynamic information on metabolism and function. Murine genetic malleability makes it a major model for heart research. The diminutive size of the mouse heart makes optical transmission studies challenging. Using a perfused murine heart center mounted in an integrating sphere for light col...
Han, Shuting Cui, Yan Helbing, Dario-Lucas
Published in
Analytical biochemistry
Inactivation of horseradish peroxidase by hydrogen peroxide (H2O2) treatment is a convenient alternative to stripping for sequential chemiluminescent western blotting (WB). However, little evidence exists on whether H2O2 treatment affects epitope recognition. Here we show that H2O2 treatment had a negligible effect for most of the tested antibodies...
Zhou, Wei Li, Yiping Song, Jinhua Li, Chenglong
Published in
Analytical biochemistry
The Hippo signaling pathway controls cell-cell contact, cell proliferation, as well as organ size by integrating changes in the cellular microenvironment. In recent years, the pivotal role of Hippo signaling in cancers has been well recognized. Inhibition of the pathway promotes the translocation of the major Hippo pathway effectors, the yes-associ...
Schultzhaus, Janna N Taitt, Chris R Orihuela, Beatriz Smerchansky, Madeline Schultzhaus, Zachary S Rittschof, Daniel Wahl, Kathryn J Spillmann, Christopher M
Published in
Analytical biochemistry
Next generation sequencing (NGS) technologies can provide an understanding of the molecular processes involved in marine fouling by Amphibalanus spp. barnacles. Here, seven methods for extracting DNA from A. amphitrite prosomata were assessed with respect to recovery, purity and size distribution. Methods incorporating organic extractions generally...
Heidari-Japelaghi, Reza Haddad, Raheem Valizadeh, Mostafa Dorani-Uliaie, Ebrahim Jalali-Javaran, Mokhtar
Published in
Analytical biochemistry
In this study, the ELP sequence was fused to human interferon-γ (hIFN-γ) and hIFN-γ-ELP fusion protein accumulated with high levels of yield and purity, compared with the corresponding unfused hIFN-γ protein. The hIFN-γ was exclusively produced in the form of insoluble inclusion bodies while the hIFN-γ was relatively soluble when expressed as an EL...
