Du, Jiale Strieter, Eric R
Published in
Analytical biochemistry
Ubiquitin chains regulate distinct signaling events through cooperative interactions with effector proteins and deubiquitinases. Measuring the strength of these interactions is often challenging; either large amounts of material are required or one of the binding partners must be labeled for detection. We sought to develop a label-free method for m...
Sun, Xiuwei Ying, Na Ju, Chuanjing Li, Zhongyi Xu, Na Qu, Guijuan Liu, Wensen Wan, Jiayu
Published in
Analytical biochemistry
In a recent study, we reported a novel assay for the detection of microRNA-21 based on duplex-specific nuclease (DSN)-assisted isothermal cleavage and hybridization chain reaction (HCR) dual signal amplification. The Fam modified double-stranded DNA products were generated after the HCR, another biotin modified probe was digested by DSN and release...
Christ, Jonas Johannes Blank, Lars Mathias
Published in
Analytical biochemistry
Polyacrylamide gel electrophoresis, being the current method of choice for length determination of inorganic polyphosphate (polyP), requires a sequencing apparatus, relies on commercially not available polyP length standards and yields only a chain length distribution. State of the art polyP quantification involves enzymatic hydrolysis of polyP to ...
Poulton, Kate Webster, Bonnie
Published in
Analytical biochemistry
Infection with Schistosoma mansoni causes intestinal schistosomiasis, a major health problem across Africa. The accurate diagnosis of intestinal schistosomiasis is vital to inform surveillance/control programs. Diagnosis mainly relies on microscopic detection of eggs in faecal samples but many factors affect sensitivity. Molecular diagnostics are s...
Silva, Gonçalo Oyekanmi, Joshua Nkere, Chukwuemeka K Bömer, Moritz Kumar, P Lava Seal, Susan E
Published in
Analytical biochemistry
Potyviruses (genus Potyvirus; family Potyviridae) are widely distributed and represent one of the most economically important genera of plant viruses. Therefore, their accurate detection is a key factor in developing efficient control strategies. However, this can sometimes be problematic particularly in plant species containing high amounts of pol...
Higgins, Owen Clancy, Eoin Forrest, Matthew S Piepenburg, Olaf Cormican, Martin Boo, Teck Wee O'Sullivan, Nicola McGuinness, Claire Cafferty, Deirdre Cunney, Robert
...
Published in
Analytical biochemistry
Recombinase polymerase amplification (RPA) is an isothermal nucleic acid amplification technology that provides rapid and robust infectious disease pathogen detection, ideal for point-of-care (POC) diagnostics in disease-prevalent low-resource countries. We have developed and evaluated three duplex RPA assays incorporating competitive internal cont...
Pan, Yong Zhan, Shenshan Xia, Fan
Published in
Analytical biochemistry
ZIF-8(zinc-methylimidazolate framework-8), one of the zeolitic imidazolate frameworks (ZIFs), as quenching platforms for detection of HIV-1 DNA, which was identified to be effective for highly sensitive detection of HIV-1 DNA. The enhanced fluorescence signal has a relationship with the ssDNA concentration, the detection limit is as low as 1.2 nmol...
Kodani, Sean D Barthélemy, Morgane Kamita, Shizuo G Hammock, Bruce Morisseau, Christophe
Published in
Analytical biochemistry
Carboxylesterases are well known for their role in the metabolism of xenobiotics. However, recent studies have also implicated carboxylesterases in regulating a number of physiological processes including metabolic homeostasis and macrophage development, underlying the need to quantify them individually. Unfortunately, current methods for selective...
Shadjou, Nasrin Hasanzadeh, Mohammad Omari, Ali
Published in
Analytical biochemistry
Rapid analyses of some water soluble vitamins (Vitamin B2, B9, and C) in commercial multi vitamins could be routinely performed in analytical laboratories. This study reports on the electropolymerization of a low toxic and biocompatible polymer "poly aspartic acid-graphene quantum dots" as a novel strategy for surface modification of glassy carbon ...
Zhang, Junjun Zhang, Wenjuan Guo, Jinjin Wang, Junchun Zhang, Yuzhong
Published in
Analytical biochemistry
In this study, a sandwich-type electrochemical immunosensor for the detection of C-reactive protein (CRP) is described. In design, Copper nanoparticles (Cu NPs) were used for signal tag and hybridization chain reaction (HCR)amplified output signal. The immunosensor fabrication involved three steps: (i) primary antibodies (Ab1) were immobilized on t...
