Fokou, Patrick Valere Tsouh Tali, Brice Mariscal Tchatat Dize, Darline Mbouna, Cedric Derick Jiatsa Ngansop, Cyrille Armel Njampa Keumoe, Rodrigue Tchokouaha, Lauve Rachel Yamthe Tchouankeu, Jean Claude Escudie, Fanny Duffy, James
...
Published in
Analytical biochemistry
Antimalarial drug discovery has been facilitated by the development of various in vitro drug susceptibility testing methods suitable for medium-throughput or high-throughput campaigns. Among many, the Plasmodium falciparum lactate dehydrogenase (PfLDH) assay has acceptable demand on equipment, labour, technical skills and affordability and offers a...
Ivancic, Valerie A Lombardo, Holly L Ma, Eugene Wikström, Mats Batabyal, Dipanwita
Published in
Analytical biochemistry
Infrared (IR) spectroscopy is rapidly gaining traction for monitoring biotherapeutic critical quality attributes. Microfluidic Modulation Spectroscopy (MMS), a novel automated IR technology, has been shown to be an effective technique for generating high quality, reproducible secondary structure data for protein therapeutics including monoclonal an...
Xie, Guoyang Zhan, Zhongxu Ye, Yu Zhou, Baoqing Tong, Ping Aguilar, Zoraida P Xu, Hengyi
Published in
Analytical biochemistry
Salmonella infection could come from eating contaminated meat or raw eggs, and drinking milk or water contaminated by Salmonella enteritidis. Therefore, it is necessary to explore a fast and easy method for the detection of S. enteritidis in these diverse samples. For this purpose, a novel particle size sensing tool was designed for ultrasensitive ...
Nice, Edouard C
Published in
Analytical biochemistry
The last decade has seen many major advances in proteomics, with over 70,000 publications in the field since 2010. A comprehensive omics toolbox has been developed facilitating rapid in depth analysis of the human proteome. Such studies are advancing our understanding of the biology of both health and disease. The combination of proteomics with oth...
Anderson, Mary E
Published in
Analytical biochemistry
This article is dedicated to the late long-time Editor-in-Chief of Analytical Biochemistry, William Jakoby. As a graduate student, I remember reading many articles in Analytical Biochemistry and Methods in Enzymology, both volumes that Bill edited. I first met him as a graduate student presenting at the American Society of Biochemistry (and Molecul...
Zon, Gerald
Published in
Analytical biochemistry
Aptamers are typically defined as relatively short (20-60 nucleotides) single-stranded DNA or RNA molecules that bind with high affinity and specificity to various types of targets. Aptamers are frequently referred to as "synthetic antibodies" but are easier to obtain, less expensive to produce, and in several ways more versatile than antibodies. T...
Delvaux, Nathan A Mathew, Basil Rice, Kevin G
Published in
Analytical biochemistry
Covalent fluorescent labels are important tools for monitoring the in vitro and in vivo localization of plasmid DNA nanoparticles, but must meet several criteria including high DNA labeling efficiencies and minimal impact on nanoparticle size. We developed a novel fluorescent labeling strategy utilizing an aryl azide photolabel conjugated to a shor...
Dorai, Thambi Pinto, John T Denton, Travis T Krasnikov, Boris F Cooper, Arthur J L
Published in
Analytical biochemistry
In rapidly dividing cells, including many cancer cells, l-glutamine is a major energy source. Utilization of glutamine is usually depicted as: l-glutamine → l-glutamate (catalyzed by glutaminase isozymes; GLS1 and GLS2), followed by l-glutamate → α-ketoglutarate [catalyzed by glutamate-linked aminotransferases or by glutamate dehydrogenase (GDH)]. ...
Cooper, Arthur J L Dorai, Thambi Pinto, John T Denton, Travis T
Published in
Analytical biochemistry
The asparaginase II pathway consists of an asparagine transaminase [l-asparagine + α-keto acid ⇆ α-ketosuccinamate + l-amino acid] coupled to ω-amidase [α-ketosuccinamate + H2O → oxaloacetate + NH4+]. The net reaction is: l-asparagine + α-keto acid + H2O → oxaloacetate + l-amino acid + NH4+. Thus, in the presence of a suitable α-keto acid substrate...
Cooper, Arthur J L Hanover, John
Published in
Analytical biochemistry
