Fresco-Cala, Beatriz López-Lorente, Ángela I Batista, Alex D Dinc, Mehmet Bansmann, Joachim Behm, R Jürgen Cárdenas, Soledad Mizaikoff, Boris
Published in
Analytical and bioanalytical chemistry
The development of synthetic particles that emulate real viruses in size, shape, and chemical composition is vital to the development of imprinted polymer-based sorbent materials (molecularly imprinted polymers, MIPs). In this study, we address surrogates for adenovirus type 5 (Adv 5) via the synthesis and subsequent modification of icosahedral gol...
Chen, Jing Yu, Bing Cong, Hailin Shen, Youqing
Published in
Analytical and bioanalytical chemistry
Membrane chromatography is mainly used for the separation and purification of proteins and biological macromolecules in the downstream processing process, also applications in sewage disposal. Membrane chromatography is recognized as an effective alternative to column chromatography because it significantly improves chromatography from affinity, hy...
Saluti, Giorgio Ricci, Matteo Castellani, Federica Colagrande, Maria Novella Di Bari, Gabriella Vulpiani, Michele Podaliri Cerasoli, Francesco Savini, Giovanni Scortichini, Giampiero D'Alterio, Nicola
...
Published in
Analytical and bioanalytical chemistry
The measure of hair cortisol concentration (HCC) is becoming an emerging approach to monitor mid-/long-term stress in animals, so it is more and more important to develop accurate and reliable methods. In the light of this, the aim of the present study was to compare mane HCCs of 47 horses with different managements, by means of an immunoassay (ELI...
Mecarelli, Enrica Aigotti, Riccardo Asteggiano, Alberto Giacobini, Paolo Chasles, Manon Tillet, Yves Dal Bello, Federica Medana, Claudio
Published in
Analytical and bioanalytical chemistry
Gonadotropin-releasing hormone isoform I (GnRH), a neuro-deca-peptide, plays a fundamental role in development and maintenance of the reproductive system in vertebrates. The anomalous release of GnRH is observed in reproductive disorder such as hypogonadotropic hypogonadism, polycystic ovary syndrome (PCOS), or following prenatal exposure to elevat...
Cavallero, Gustavo J Wang, Yan Nwosu, Charles Gu, Sheng Meiyappan, Muthuraman Zaia, Joseph
Published in
Analytical and bioanalytical chemistry
Recombinant protein engineering design affects therapeutic properties including protein efficacy, safety, and immunogenicity. Importantly, glycosylation modulates glycoprotein therapeutic pharmacokinetics, pharmacodynamics, and effector functions. Furthermore, the development of fusion proteins requires in-depth characterization of the protein inte...
Bruns, Stefan Wienhausen, Gerrit Scholz-Böttcher, Barbara Wilkes, Heinz
Published in
Analytical and bioanalytical chemistry
B vitamins have high microbiological relevance in the marine environment, but their very low concentrations and the chemical heterogeneity of the individual vitamins make their analysis challenging. Mass spectrometric analysis of B vitamins in environmental samples at trace levels has mainly been performed using triple quadrupole mass spectrometers...
Meikopoulos, Thomas Begou, Olga Panagoulis, Theodoros Kontogiannidou, Eleni Fatouros, Dimitrios G Miller, John H Theodoridis, Georgios Gika, Helen
Published in
Analytical and bioanalytical chemistry
A new method was developed and validated for the simultaneous determination of nicotine and tobacco-specific nitrosamines (TSNAs) 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and N-nitrosonornicotine (NNN) in two different tests matrices: porcine buccal epithelium tissue and phosphate buffered saline (PBS) extracts of smokeless tobacco prod...
Zelzer, Sieglinde Meinitzer, Andreas Enko, Dietmar Herrmann, Markus Goessler, Walter
Published in
Analytical and bioanalytical chemistry
Different foods, especially mushrooms, are a valuable source of vitamin D2. However, published concentrations in mushrooms show large variabilities. One reason for this is certainly the high biological variability caused by growth conditions, and another could also be found in the analytical methodology. Therefore, this study aimed to develop a sen...
Ražić, Slavica Segundo, Marcela A. Turner, Diane Miró, Manuel Baeumner, Antje J.
Published in
Analytical and Bioanalytical Chemistry
Zhou, Hongyu Bu, Shengjun Xu, Yao Xue, Lulu Li, Zhongyi Hao, Zhuo Wan, Jiayu Tang, Feng
Published in
Analytical and Bioanalytical Chemistry
This study provides proof of concept of a colorimetric biosensor for influenza H1N1 virus assay based on the CRISPR/Cas13a system and hybridization chain reaction (HCR). Target RNA of influenza H1N1 virus activated the trans-cleavage activity of Cas13a, which cleaved the special RNA sequence (-UUU-) of the probe, further initiating HCR to copiously...
