Chen, Weili Yu, Hojeong Sun, Fu Akid Ornob Brisbin, Ryan Ganguli, Anurup Vemuri, Vinay Strzebonski, Piotr Cui, Guangzhe Allen, Karen J.
...
Published in
Analytical Chemistry
New tools are needed to enable rapid detection, identification, and reporting of infectious viral and microbial pathogens in a wide variety of point-of-care applications that impact human and animal health. We report the design, construction, and characterization of a platform for multiplexed analysis of disease-specific DNA sequences that utilizes...
Hassan, Sammer-ul Morgan, Hywel Zhang, Xunli Niu, Xize
Published in
Analytical Chemistry
High-throughput, quantitative, and rapid microfluidic-based separations has been a long-sought goal for applications in proteomics, genomics, biomarker discovery, and clinical diagnostics. Using droplet-interfaced microchip electrophoresis (MCE) techniques, we have developed a novel parallel MCE platform, based on the concept of combining the Slipc...
Published in
Analytical Chemistry
Electrografted diazonium salt layers on the surface of surface plasmon resonance (SPR) sensors present potential for a significant improvement in antifouling coatings. A pulsed potential deposition profile was used in order to circumvent mass-transport limitations for layer deposition rate. The influence of number of pulses with respect to antifoul...
Maiyalagan, Thandavarayan Kannan, Palanisamy Jönsson-Niedziolka, Martin Niedziolka-Jönsson, Joanna
Published in
Analytical Chemistry
Supported tungsten carbide is an efficient and vital nanomaterial for the development of high-performance, sensitive, and selective electrochemical sensors. In this work, tungsten carbide with tube-like nanostructures (WC NTs) supported platinum nanoparticles (PtNPs) are synthesized and explored as an efficient catalyst toward electrochemical oxida...
Li, Guoyun Cai, Chao Li, Lingyun Fu, Li Chang, Yuqing Zhang, Fuming Toida, Toshihiko Xue, Changhu Linhardt, Robert J
Published in
Analytical Chemistry
Heparin is a critically important anticoagulant drug that was contaminated with a persulfonated polysaccharide in 2008, resulting in a number of severe adverse reactions, some leading to death. Controversy remains as to the precise composition of the 2008 contaminant, and new information suggests that heparin may now be subject to adulteration with...
Yoshioka, Kyoko Kato, Dai Kamata, Tomoyuki Niwa, Osamu
Published in
Analytical Chemistry
The development of a biocatalytic electrode consisting of cytochrome P450 (CYP) proteins would be a key technology with which to establish simple drug metabolizing biosensors or screening devices for drug inhibitors. We have successfully detected the direct electron transfer (DET) from a human CYP layer or a CYP microsome adsorbed on a bare indium ...
Chang, Yuqing Cai, Chao Li, Lingyun Miao, Jianjun Ucakturk, Ebru Li, Guoyun Ly, Mellisa Linhardt, Robert J
Published in
Analytical Chemistry
Rapid and highly sensitive detection of the carbohydrate components of glycoconjugates is critical for advancing glycobiology. Fluorescence (or Förster) resonance energy transfer (FRET) is commonly used in detection of DNA, in protein structural biology, and in protease assays but is less frequently applied to glycan analysis due to difficulties in...
Matthäus, Christian Krafft, Christoph Dietzek, Benjamin Brehm, Bernhard R Stefan Lorkowski Popp, Jürgen
Published in
Analytical Chemistry
Monocyte-derived macrophages play a key role in atherogenesis because their transformation into foam cells is responsible for deposition of lipids in plaques within arterial walls. The appearance of cytosolic lipid droplets is a hallmark of macrophage foam cell formation, and the molecular basics involved in this process are not well understood. Of...
Sadeghi, Saman Ding, Huijiang Shah, Gaurav J. Chen, Supin Keng, Pei Yuin Kim, Chang-Jin “cj” R. Michael van Dam
Published in
Analytical Chemistry
Zhao, Chao Yin, Ruichuan Yin, Junfa Zhang, Dapeng Wang, Hailin
Published in
Analytical Chemistry
A capillary monolithic bioreactor of snake venom phosphodiesterase (SVP) was constructed to generate different single-nucleotide mass ladders of oligodeoxynucleotides for mass spectrometry (MS)-based sequencing by immobilization. The immobilization of SVP in the porous silica monolith significantly enhances its stability for prolonged and repeated ...
