Eissa, Shimaa Zourob, Mohammed
Published in
Analytical Chemistry
Collection of nasopharyngeal samples using swabs followed by the transfer of the virus into a solution and an RNA extraction step to perform reverse transcription polymerase chain reaction (PCR) is the primary method currently used for the diagnosis of COVID-19. However, the need for several reagents and steps and the high cost of PCR hinder its wo...
Eriksson, Jonatan O Sánchez Brotons, Alejandro Rezeli, Melinda Suits, Frank Markó-Varga, György Horvatovich, Peter
Published in
Analytical chemistry
Mass spectrometry imaging (MSI) is a technique that provides comprehensive molecular information with high spatial resolution from tissue. Today, there is a strong push toward sharing data sets through public repositories in many research fields where MSI is commonly applied; yet, there is no standardized protocol for analyzing these data sets in a...
Pang, Bo Xu, Jingyang Liu, Yanming Peng, Hanyong Feng, Wei Cao, Yiren Wu, Jinjun Xiao, Huyan Pabbaraju, Kanti Tipples, Graham
...
Published in
Analytical chemistry
We have developed a single-tube assay for SARS-CoV-2 in patient samples. This assay combined advantages of reverse transcription (RT) loop-mediated isothermal amplification (LAMP) with clustered regularly interspaced short palindromic repeats (CRISPRs) and the CRISPR-associated (Cas) enzyme Cas12a. Our assay is able to detect SARS-CoV-2 in a single...
Zhou, Haiwei Liu, Donglai Ma, Liang Ma, Tingting Xu, Tingying Ren, Lili Li, Liang Xu, Sihong
Published in
Analytical chemistry
The outbreak of novel coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread worldwide. To meet the urgent and massive demand for the screening and diagnosis of infected individuals, many in vitro diagnostic assays using nucleic acid tests (NATs) have been urgently authorized by regulat...
Hu, Keke Nguyen, Tho D. K. Rabasco, Stefania Oomen, Pieter E. Ewing, Andrew G.
Published in
Analytical Chemistry
Wörner, Tobias P. Shamorkina, Tatiana M. Snijder, Joost Heck, Albert J. R.
Published in
Analytical Chemistry
V D Dos Santos, A Catarina Heydenreich, Rosa Derntl, Christian Mach-Aigner, Astrid R Mach, Robert L Ramer, Georg Lendl, Bernhard
Published in
Analytical chemistry
Determination of the intracellular location of proteins is one of the fundamental tasks of microbiology. Conventionally, label-based microscopy and super-resolution techniques are employed. In this work, we demonstrate a new technique that can determine intracellular protein distribution at nanometer spatial resolution. This method combines nanosca...
Tian, Xiaobo de Vries, Marcel P. Permentier, Hjalmar P. Bischoff, Rainer
Published in
Analytical Chemistry
Quantifying proteins based on peptide-coupled reporter ions is a multiplexed quantitative strategy in proteomics that alleviates the problem of ratio distortion caused by peptide cofragmentation, as commonly observed in other reporter-ion-based approaches, such as TMT and iTRAQ. Data-independent acquisition (DIA) is an attractive alternative to dat...
Glenne, Emelie Leśko, Marek Samuelsson, Jörgen Fornstedt, Torgny
Published in
Analytical chemistry
In supercritical fluid chromatography (SFC), the retention of a solute depends on the temperature, density, pressure, and cosolvent fraction. Here, we investigate how the adsorption of the cosolvent MeOH changes with pressure and temperature and how this affects the retention of several solutes. The lower the pressure, the stronger the MeOH adsorpt...
Podrażka, Marta Witkowska Nery, Emilia Henares, Terence G Jönsson-Niedziółka, Martin Arrigan, Damien W M
Published in
Analytical chemistry
We present a new electrochemical system that combines paper-based sensing and ion-transfer voltammetry, bringing the latter a step closer toward point-of-care applications. Studies at the interface between two immiscible electrolyte solutions (ITIES) are often performed to detect redox-inactive species; unfortunately, due to the inherent instabilit...