Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni 2+ magnetic beads
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Published in Analytical Biochemistry
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Ultra-fast, high throughput and inexpensive detection of SARS-CoV-2 seroconversion using Ni 2+ magnetic beads
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Novel approaches for rapid detection of COVID-19 during the pandemic: A review
Published in Analytical Biochemistry
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Validation of LC-MS/MS methods for determination of remdesivir and its metabolites GS-441524 and GS-704277 in acidified ...
Published in Analytical Biochemistry
Remdesivir (RDV) is a phosphoramidate prodrug designed to have activity against a broad spectrum of viruses. Following IV administration, RDV is rapidly distributed into cells and tissues and simultaneously metabolized into GS-441524 and GS-704277 in plasma. LC-MS/MS methods were validated for determination of the 3 analytes in human plasma that in...
Small molecular fluorescence dyes for immuno cell analysis
Published in Analytical Biochemistry
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Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification.
Published in Analytical biochemistry
This study investigated a method using loop-mediated isothermal amplification (LAMP) for the rapid detection of cnm-positive Streptococcus mutans (S. mutans) associated with cerebral microhemorrhage. LAMP amplified the cnm gene plasmid vector, but not human or microbial genomic DNA. The cnm DNA of the cnm-positive S. mutans strain was detected in s...
Recent advances in aptamer applications for analytical biochemistry
Published in Analytical Biochemistry
• Aptamer publications indexed in PubMed during 2019–April 2020 number ~ 2000. • Bioanalytical applications of these recent publications were analyzed. • Representative examples are given from 9 categories of these recent bioanalytical applications of aptamers.
Identifying transglutaminase reaction products via mass spectrometry as exemplified by the MUC2 mucin - Pitfalls and tra...
Published in Analytical biochemistry
In order to demonstrate transglutaminase activity in biological samples immunological as well as glutamine- and amine-donor based assays are commonly used. However, the identification of the transglutaminase reaction product, i. e. the isopeptide cross-linked peptides/proteins or the deamidated protein/peptide are often neglected. This article desc...
Lab-scale preparation and QC of phytase assay substrate from rice bran.
Published in Analytical biochemistry
Phytases are involved in the phosphate acquisition and remobilization in plants, microbes and animals. They have become important technical enzymes in the feed industry and are used to make phosphate, present in animal feed as phytate, available for monogastric animal nutrition. Phytases may also be beneficial to human nutrition because phytate is ...
Development of an HPLC-based guanosine monophosphate kinase assay and application to Plasmodium vivax guanylate kinase.
Published in Analytical biochemistry
The development of a high-performance liquid chromatography (HPLC)-based method, for guanosine monophosphate kinase activity assays, is presented. The method uses the intrinsic UV absorption (at 260 nm) of substrates and products of the enzymatic reaction (GMP, ATP, ADP and GDP) to unambiguously determine percent conversion of substrate into produc...
Preparation of stable tau oligomers for cellular and biochemical studies.
Published in Analytical biochemistry
Increasing evidence suggests that small oligomers are the principal neurotoxic species of tau in Alzheimer's disease and other tauopathies. However, mechanisms of tau oligomer-mediated neurodegeneration are poorly understood. The transience of oligomers due to aggregation can compromise the stability of oligomers prepared in vitro. Consequently, we...
