Xiao, Deqing John Ling, Kah Hiing Tarnowski, Thomas Humeniuk, Rita German, Polina Mathias, Anita Chu, Jasper Chen, Yuan-Shek van Ingen, Eric
Published in
Analytical Biochemistry
Remdesivir (RDV) is a phosphoramidate prodrug designed to have activity against a broad spectrum of viruses. Following IV administration, RDV is rapidly distributed into cells and tissues and simultaneously metabolized into GS-441524 and GS-704277 in plasma. LC-MS/MS methods were validated for determination of the 3 analytes in human plasma that in...
Kitagawa, Masae Nagamine, Kentaro Oka, Hiroko Ouhara, Kazuhisa Ogawa, Ikuko Komatsuzawa, Hitoshi Kurihara, Hidemi
Published in
Analytical biochemistry
This study investigated a method using loop-mediated isothermal amplification (LAMP) for the rapid detection of cnm-positive Streptococcus mutans (S. mutans) associated with cerebral microhemorrhage. LAMP amplified the cnm gene plasmid vector, but not human or microbial genomic DNA. The cnm DNA of the cnm-positive S. mutans strain was detected in s...
Zon, Gerald
Published in
Analytical Biochemistry
• Aptamer publications indexed in PubMed during 2019–April 2020 number ~ 2000. • Bioanalytical applications of these recent publications were analyzed. • Representative examples are given from 9 categories of these recent bioanalytical applications of aptamers.
Arike, Liisa Hansson, Gunnar C Recktenwald, Christian V
Published in
Analytical biochemistry
In order to demonstrate transglutaminase activity in biological samples immunological as well as glutamine- and amine-donor based assays are commonly used. However, the identification of the transglutaminase reaction product, i. e. the isopeptide cross-linked peptides/proteins or the deamidated protein/peptide are often neglected. This article desc...
Madsen, Claus Krogh Brearley, Charles Alistair Brinch-Pedersen, Henrik
Published in
Analytical Biochemistry
• Sodium phytate suitable for assays was prepared from rice bran. • The purification require no special equipment and can be performed in most biochemistry labs. • Directions are given for quality control of the product.
Pedro, Liliana Cross, Megan Hofmann, Andreas Mak, Tin Quinn, Ronald J
Published in
Analytical biochemistry
The development of a high-performance liquid chromatography (HPLC)-based method, for guanosine monophosphate kinase activity assays, is presented. The method uses the intrinsic UV absorption (at 260 nm) of substrates and products of the enzymatic reaction (GMP, ATP, ADP and GDP) to unambiguously determine percent conversion of substrate into produc...
Karikari, Thomas K Nagel, David A Grainger, Alastair Clarke-Bland, Charlotte Hill, Eric J Moffat, Kevin G
Published in
Analytical biochemistry
Increasing evidence suggests that small oligomers are the principal neurotoxic species of tau in Alzheimer's disease and other tauopathies. However, mechanisms of tau oligomer-mediated neurodegeneration are poorly understood. The transience of oligomers due to aggregation can compromise the stability of oligomers prepared in vitro. Consequently, we...
Fujita, Motomichi Adachi, Koji Nagasawa, Michiaki
Published in
Analytical biochemistry
The group of positive-sense single-stranded RNA ((+) ssRNA) viruses includes many important human pathogens. However, specific antiviral agents are not currently available for many RNA viruses. For screening of antiviral agents, methods that are simple, rapid, and compatible with high-throughput are required. Here, we describe a novel method for me...
Hampf, Mathias Gossen, Manfred
Published in
Analytical Biochemistry
We established a quantitative reporter gene protocol, the P/Rluc assay system, allowing the sequential measurement of Photinus and Renilla luciferase activities from the same extract. Other than comparable commercial reporter assay systems and their noncommercial counterparts, the P/Rluc assay system was formulated under the aspect of full compatib...
Lyon, Sarah M Mayampurath, Anoop Rogers, M Rose Wolfgeher, Donald J Fisher, Sean M Volchenboum, Samuel L He, Tong-Chuan Reid, Russell R
Published in
Analytical Biochemistry
The presence of the dense hydroxyapatite matrix within human bone limits the applicability of conventional protocols for protein extraction. This has hindered the complete and accurate characterization of the human bone proteome thus far, leaving many bone-related disorders poorly understood. We sought to refine an existing method of protein extrac...
