Chan, Clarence W Kwon, Seunghyug Matushek, Scott M Ciaglia, Carol Bethel, Cindy Beavis, Kathleen G
Published in
American Journal of Clinical Pathology
Objectives To report our institutional experience in devising and implementing a pooling protocol and process for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) reverse transcription polymerase chain reaction (RT-PCR) testing over a 3-month period in the fall of 2020. Methods The widespread testing implemented in the United States for...
Mirza, Kamran M Gonzalez, Raul S Jiang, Xiaoyin “Sara” Khanafshar, Elham Wobker, Sara E
Published in
American Journal of Clinical Pathology
Objectives The goal is to describe the use of a virtual platform in the delivery of Virtual Pathology Grand Rounds (VPGR) and discuss the overall experience from the perspective of hosts, speakers, and participants. Methods Zoom was a natural choice for an online format because virtual platforms had been increasingly used to conduct meetings and me...
Buchan, Blake W Gerstbrein, Derek Cruz, Amorina Hoff, Jess Sievert, Emily Ledeboer, Nathan A Faron, Matthew L
Published in
American Journal of Clinical Pathology
Objectives We conducted an analytic and clinical comparison of a novel high-definition polymerase chain reaction PCR (HDPCR) assay to traditional real-time PCR (RT-PCR) for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in upper respiratory specimens. Methods Analytic performance of RT-PCR, HDPCR, and extraction-free ...
Chornenkyy, Yevgen Mejia-Bautista, Melissa Brucal, Melanie Blanke, Timothy Dittmann, David Yeldandi, Anjana Boike, Justin R Lomasney, Jon W Nayar, Ritu Jennings, Lawrence J
...
Published in
American Journal of Clinical Pathology
Objectives The novel coronavirus, severe acute respiratory syndrome coronavirus 2, causing coronavirus disease 2019 (COVID-19) remains a global health threat and a significant source of human morbidity and mortality. While the virus primarily induces lung injury, it also has been reported to cause hepatic sequelae. Methods We aimed to detect the vi...
Geisler, Daniel Freeman, Megan Culler Rapsinski, Glenn J Wheeler, Sarah E
Published in
American Journal of Clinical Pathology
Objectives Serologic assay performance studies for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in pediatric populations are lacking, and few seroprevalence studies have routinely incorporated orthogonal testing to improve accuracy. Methods Remnant serum samples for routine bloodwork from 2,338 pediatric patients at UPMC Children’s...
Xu, Jing Kirtek, Timothy Xu, Yan Zheng, Hui Yao, Huiyu Ostman, Emily Oliver, Dwight Malter, James S Gagan, Jeffrey R SoRelle, Jeffrey A
...
Published in
American Journal of Clinical Pathology
Objectives The Bio-Rad SARS-CoV-2 ddPCR Kit (Bio-Rad Laboratories) was the first droplet digital polymerase chain reaction (ddPCR) assay to receive Food and Drug Administration (FDA) Emergency Use Authorization approval, but it has not been evaluated clinically. We describe the performance of ddPCR—in particular, its ability to confirm weak-positiv...
De Las Casas, Luis E Hicks, David G
Published in
American Journal of Clinical Pathology
Objectives Tumor biomarker analyses accompanying immuno-oncology therapies are coupled with a tumor tissue journey aiming to guide tissue procurement and allow for accurate diagnosis and delivery of test results. The engagement of pathologists in the tumor tissue journey is essential because they are able to link the preanalytic requirements of thi...
Yi, Xin Chan, Clarence W Yeo, Kiang-Teck J
Published in
American journal of clinical pathology
Samuelson, Megan I Chen, Stephanie J Boukhar, Sarag A Schnieders, Eric M Walhof, Mackenzie L Bellizzi, Andrew M Robinson, Robert A Rajan KD, Anand
Published in
American Journal of Clinical Pathology
Objectives The ongoing global severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic necessitates adaptations in the practice of surgical pathology at scale. Primary diagnosis by whole-slide imaging (WSI) is a key component that would aid departments in providing uninterrupted histopathology diagnosis and maintaining revenue streams ...
Harris, Cynthia K Hung, Yin P Nielsen, G Petur Stone, James R Ferry, Judith A
Published in
American Journal of Clinical Pathology
Objectives Coronavirus disease 19 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is associated with diverse clinical, including hematologic, abnormalities. We describe peripheral blood and bone marrow findings in deceased and living patients with COVID-19. Methods We examined bone marrows from 20 autopsies and 2...
