Nyolczas, Noémi Dékány, Miklós Muk, Balázs Szabó, Barna
Published in
Advances in experimental medicine and biology
The use of direct acting vasodilators (the combination of hydralazine and isosorbide dinitrate -Hy+ISDN-) in heart failure with reduced ejection fraction (HFrEF) is supported by evidence, but rarely used.However, treatment with Hy+ISDN is guideline-recommended for HFrEF patients who cannot receive either angiotensin-converting enzyme inhibitors or ...
Witek, Bożena Rochon-Szmejchel, Danuta Stanisławska, Iwona Łyp, Marek Wróbel, Krzysztof Zapała, Arkadiusz Kamińska, Agnieszka Kołątaj, Adam
Published in
Advances in experimental medicine and biology
The study investigated a panel of lysosomal enzymes in the liver and kidney tissues in alloxan-induced diabetes in the mouse. The mice were divided into six experimental groups receiving 10% alloxan at a dose of 50 and 75 mg/kg over a period of four, eight, and twelve days; each group was compared with controls receiving 0.9% NaCl. The findings wer...
Kaushik, Stuti Saha, Rumpa Das, Shukla Ramachandran, V G Goel, Ashish
Published in
Advances in experimental medicine and biology
Diarrhea is a debilitating condition in HIV infected individuals and with the finding that almost 1/4 cases of diarrhea in HIV are due to microsporidia, there is a dire need to institute measures for its detection on a regular basis. Keeping this in mind the study aims to determine the burden of intestinal microsporidiosis in HIV seropositive patie...
Szwed, A John, A Goździk-Spychalska, J Czaiński, W Czerniak, W Ratajczak, J Batura-Gabryel, H
Published in
Advances in experimental medicine and biology
The purpose of the study was to evaluate the influence of nutrition and of the severity of mutation type on survival rate in cystic fibrosis (CF) patients. Data were longitudinally collected from 60 hospitalized adult CF patients, aged 18-50. The variables consisted of body mass index (BMI) ratio, Cole's BMI cut-off points, severity of mutation typ...
Singh, Rani Alex, Rani Singh, Umesh Kumar, Sushil Sengar, Gyanendra Singh Raja, T V Alyethodi, R R Kumar, Ashish Deb, Rajib
Published in
Advances in experimental medicine and biology
Integrins are one of the major biologically active proteins responsible for Foot-and-mouth disease virus (FMDV)- host interaction. Out of various heterodimeric integrins discovered, αVβ6 heterodimer serves as the chief receptor for FMDV host tropism. Earlier studies reported that, SNPs at beta 6 subunit (ITGB6) were associated with the occurrence o...
Luo, Xuerui Zhu, Fuxiang Chen, Zuojia Li, Dan Li, Bin
Published in
Advances in experimental medicine and biology
T cells play a critical role to defend against tumor and maintain immune homeostasis. The diverse functions of T cells require precise regulation of metabolic pathways. Recent studies reveal that metabolic changes are tightly linked to the activation and function of T cells. Given the importance of these cells in tumor progression, it is important ...
Guest, Paul C Rahmoune, Hassan
Published in
Advances in experimental medicine and biology
A major challenge in proteomic biomarker discovery and validation for psychiatric diseases is the inherent biological complexity underlying these conditions. There are also many technical issues which hinder this process such as the lack of standardization in sampling, processing and storage of bio-samples in preclinical and clinical settings. This...
Stephen, Laurie Schwarz, Emanuel Guest, Paul C
Published in
Advances in experimental medicine and biology
Multiplex immunoassays allow for the rapid profiling of biomarker proteins in biological fluids, using less sample and labour than in single immunoassays. This chapter details the methods to develop and manufacture a 5-plex immunoassay for the Luminex® platform. Although assay development is not included here, the same methods can be used to covale...
Rahmoune, Hassan Guest, Paul C
Published in
Advances in experimental medicine and biology
Peripheral blood mononuclear cells (PBMCs) have been used as a surrogate model of brain function in studies of psychiatric disorders, such as schizophrenia. This chapter describes the preparation of PBMCs from whole blood using a density gradient/cell culture protocol. This includes collection of the PBMC culture media and preparation of cell extra...
Krishnamurthy, Divya Rahmoune, Hassan Guest, Paul C
Published in
Advances in experimental medicine and biology
Psychiatric disorders have been associated with perturbations of the hypothalamic-pituitary-adrenal axis. Therefore, proteomic studies of the pituitary gland have the potential to provide new insights into the underlying pathways affected in these conditions as well as identify new biomarkers or targets for use in developing improved medications. T...
