Bakli, Mahfoud Karim, Loukmane Mokhtari-Soulimane, Nassima Merzouk, Hafida Vincent, Florence
Published in
3 Biotech
Glycosyltransferases (GTs) are widely present in several organisms. These enzymes specifically transfer sugar moieties to a range of substrates. The processes of bacterial glycosylation of the cell wall and their relations with host–pathogen interactions have been studied extensively, yet the majority of mycobacterial GTs involved in the cell wall ...
Dutta, Suman Muthusamy, Vignesh Chhabra, Rashmi Zunjare, Rajkumar U. Hossain, Firoz
Published in
3 Biotech
A modified SDS–Trizol method was optimized for isolation of total RNA from the stored maize seeds at regular interval of one month for 4 months. Use of SDS extraction buffer before the use of Trizol reduced the co-precipitation problem associated with high carbohydrate content in the seed. Recorded mean RNA yield from seeds across the storage inter...
Mishra, Divya Mishra, Ashish Chaturvedi, Vivek K. Singh, M. P.
Published in
3 Biotech
SARS-CoV-2, a novel Betacoronavirus, has attracted global attention because of its extremely high infection rate and large number of human deaths. It possesses a positive-sense, single-stranded RNA of ~ 30 kb nucleotides as its genetic material. It is responsible for COVID-19 which has been declared a pandemic by WHO. Having reported for the first ...
Verma, Vikas Joshi, Vinay G. Ranjan, Puneet Tomar, Piyush Chhabra, Rajesh Mahajan, N. K. Goel, Parveen
Published in
3 Biotech
The objective of the study was to develop a bio-safe synthetic peptide ELISA for the detection of antibodies against the infectious bronchitis virus (IBV) using a novel multiple antigenic peptide approach (MAP). After initial ELISA optimization, diagnostic sensitivity (DSn) and specificity (DSp) for the linear peptides were determined using receive...
Bohra, Abhishek Jha, Rintu Lamichaney, Amrit Singh, Deepak Jha, Uday C. Naik, S. J. Satheesh Datta, Dibendu Maurya, Alok K. Tiwari, Abha Yadav, Vivekanand
...
Published in
3 Biotech
Seed traits present important breeding targets for enhancing grain yield and quality in various grain legume crops including pigeonpea. The present study reports significant genetic variation for six seed traits including seed length (SL), seed width (SW), seed thickness (ST), seed weight (SWT), electrical conductivity (EC) and water uptake (WU) am...
Figueroa-Torres, L. A. Lizardi-Jiménez, M. A. López-Ramírez, N. Varela-Santos, E. C. Hernández-Rosas, F. Favela-Torres, E. Hernández-Martínez, R.
Published in
3 Biotech
In the present work, bioethanol was produced by sugar fermentation obtained from water hyacinth using a novelty hybrid method composed of steam explosion and enzymatic hydrolysis, using hydrolytic enzymes produced by solid-state fermentation and water hyacinth as substrate. The highest activity, 42 U for xylanase and 2 U for cellulase per gram of d...
Sharma, Priya Kumar, Vineet Khosla, Rajiv Guleria, Praveen
Published in
3 Biotech
Key messageNaringenin exposure altered auxin redistribution via VrPIN1 leading to morphological alterations and significantly reduced the protein precipitable tannins that further enhanced the protein accumulation and bioavailability.Flavonoid exposure is known to affect the antioxidant profile of legumes. However, a detailed study evaluating the e...
Wang, Mou Ai, Li Zhang, Mengping Wang, Fengqing Wang, Chuan
Published in
3 Biotech
A esterase gene was characterized from a halophilic bacterium Chromohalobacter canadensis which was originally isolated from a salt well mine. Sequence analysis showed that the esterase, named as EstSHJ2, contained active site serine encompassed by a conserved pentapeptide motif (GSSMG). The EstSHJ2 was classified into a new lipase/esterase family ...
Raj, Rashmi Kaur, Charanjeet Srivastava, Ashish Kumar, Susheel Raj, S. K.
Published in
3 Biotech
Potyvirus species associated with yellow leaf stripe disease of Indian narcissus (Narcissus tazetta L.) var. Paperwhite has been studied by sequence analyses of ~ 1.5 kb genomic fragments obtained from seven RT-PCR amplifications of infected samples. Sequence analysis revealed the occurrence of three potyvirus species: cyrtanthus elatus virus-A (CE...
Qian, Weidong Sun, Zhaohuan Fu, Yuting Yang, Min Wang, Ting Li, Yongdong
Published in
3 Biotech
Staphylococcus aureus and Staphylococcus lugdunensis are often associated with pathogenic biofilms ranging from superficial mucosal to life-threatening systemic infections. Recent studies have reported that chelerythrine (CHE) displays antimicrobial activities against a few microorganisms, but its effects on dual-species biofilms of S. aureus and S...
