Zhen, Jie Tan, Ming Fu, Xiaoping Shu, Wenju Zhao, Xingya Yang, Shibin Xu, Jianyong Ma, Yanhe Zheng, Hongchen Song, Hui
...
Published in
3 Biotech
A high heterologous expression of an alkaline pectate lyase (APL) pelNK93I in E. coli was obtained through optimizing the lactose feeding and fed-batch fermentation. The highest soluble APL activity produced by E. coli BL21 (pET22b-pelNK93I) was 10,181 U/mL which is the highest level so far. On this basis, to improve the extracellular yield of APL,...
Mahboubi, Mojgan Mehrabi, Rahim Naji, Amir Mohammad Talebi, Reza
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3 Biotech
In this study, 129 wheat genotypes from globally diverse origins were genotyped using DArTseq (SilicoDArT and SNP) markers. After filtering markers for quality-filtering, 14,270 SilicoDArTs and 6484 SNPs were retained and used for genetic diversity, population structure and linkage disequilibrium analyses. The highest number of SilicoDArT and SNP m...
Kumar, Pasupuleti Santhosh Srikanth, Lokanathan Reddy, K. Sudheer Sarma, Potukuchi Venkata Gurunadha Krish...
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3 Biotech
In the present study, we investigated the frequency of BRCA1 gene mutations in 30 breast cancer (BC) patients of independent family history and 30 healthy control subjects. The immunohistochemistry (IHC) of BC patients showed duct cell carcinoma and distinct expression of the human epidermal growth factor receptor 2 (HER2). The genomic DNA was extr...
Ha, Duc Danh Nguyen, Oanh Thi
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3 Biotech
In this work, Thauera sp. DO isolated from sludge and sediment utilized p-chlorocresol and some related compounds as the sole carbon and energy sources under both aerobic and anaerobic conditions. The pathways for p-chlorocresol in the isolate under each condition were different. Under the aerobic condition, p-chlorocresol was degraded via two sepa...
Long, Chuannan Cui, Jingjing Xie, Shaobin Zhang, Dongsheng Liu, Mengmeng Zhang, Zhe Huang, Zhiwei Zeng, Bin
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3 Biotech
Previously, AoamyA, the alpha amylase-encoding gene from Aspergillus oryzae, was heterologously expressed in Monascus ruber CICC41233 to promote starch hydrolysis and increase the production of Monascus pigments. The target of this study is to screen the effective alpha-amylases from M. ruber for starch fast degradation and investigated for Monascu...
Sritrakul, Nipat Nitisinprasert, Sunee Keawsompong, Suttipun
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3 Biotech
Hydrolysis products of defatted copra meal (DCM) hydrolysis were investigated with either recombinant β-mannanases from Klebsiella oxytoca KUB-CW2-3 (KMAN-3) or Bacillus circulans NT 6.7 (MAN 6.7). Morphological changes and functional groups of solid residues were also determined by scanning electron microscopy (SEM) and Fourier transform infrared ...
Nanda, Harshit Ponnusamy, Nirmaladevi Odumpatta, Rajasree Jeyakanthan, Jeyaraman Mohanapriya, Arumugam
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3 Biotech
Psoriasis is a chronic inflammatory disease causing itching in the body and pain in the joints. Currently, no permanent cure is available at a commercial level for this disease. Genome wide association studies (GWAS) provide a deeper insight that helps in better understanding this disease and further possible cure of this disease. The major goal of...
Rathore, Himanshi Prasad, Shalinee Sehwag, Sneha Sharma, Satyawati
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3 Biotech
Calocybe indica mushroom was exposed under natural and artificial UVB light to enhance vitamin D2 contents in the fruit bodies. When Kinetic model was designed to examine the rate of conversion of ergosterol into vitamin D2 at different time intervals (0, 15, 45, 60 and 90 min), it was found that the conversion was linear with time. The maximum con...
Bidja Abena, Marie Thérèse Chen, Guoqing Chen, Zeyu Zheng, Xiucheng Li, Shanshan Li, Tongtong Zhong, Weihong
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3 Biotech
This study investigated the impacts of crude oil, diesel, and gasoline on the diversity of indigenous microbial communities as well as culturable microorganisms in the studied soil. Oil contamination led to shifts in the diversity of the soil’s microbial communities, regardless of the contaminant applied. Unpolluted soils were more diverse and even...
Fatima, Bilqees Javed, Muhammad Mohsin
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3 Biotech
Cell lysate of Escherichia coli strain BL21 showed significant D-glucose isomerase activity. The rate of glucose conversion was increased up to 40% when cells were induced with 1% D-xylose. E. coli BL21 xylose isomerase (ECXI-BL21) was purified to homogeneity, up to 1.9-fold with overall 10.88% enzyme yield by heat shock, salting out and electro-el...
