Yoshihiro Izumiya

Kaposi s Sarcoma-associated Herpesvirus (KSHV) Encodes a SUMO E3 ligase That Is SIM-dependent and SUMO-2/3-specific

Published in Journal of Biological Chemistry

Homodimerization of Marek s Disease Virus-Encoded Meq Protein Is Not Sufficient for Transformation of Lymphocytes in Chi...

Published in Journal of Virology

Kruppel-Associated Box Domain-Associated Protein-1 as a Latency Regulator for Kaposi s Sarcoma-Associated Herpesvirus an...

Published in Cancer Research

Regulation of Id1 Expression by Src: Implications for Targeting of the Bone Morphogenetic Protein Pathway in Cancer

Published in Cancer Research

Kaposi s sarcoma-associated herpesvirus-encoded protein kinase and its interaction with K-bZIP

Published in Journal of Virology

Kaposi’s sarcoma-associated Herpesvirus K-bZIP represses gene transcription via SUMO modification

Published in Journal of Virology

Kaposi s sarcoma-associated herpesvirus K-Rta exhibits SUMO-targeting ubiquitin ligase (STUbL) like activity and is esse...

Published in PLoS Pathogens

The small ubiquitin-like modifier (SUMO) is a protein that regulates a wide variety of cellular processes by covalent attachment of SUMO moieties to a diverse array of target proteins. Sumoylation also plays an important role in the replication of many viruses. Previously, we showed that Kaposi s sarcoma-associated herpesvirus (KSHV) encodes a SUMO...

Marek s disease virus Meq transforms chicken cells via the v-Jun transcriptional cascade: a converging transforming path...

Published in Proceedings of the National Academy of Sciences

Marek s disease virus (MDV) is a highly pathogenic and oncogenic herpesvirus of chickens. MDV encodes a basic leucine zipper (bZIP) protein, Meq (MDV EcoQ). The bZIP domain of Meq shares homology with Jun/Fos, whereas the transactivation/repressor domain is entirely different. Increasing evidence suggests that Meq is the oncoprotein of MDV. Direct ...

Biphasic euchromatin-to-heterochromatin transition on the KSHV genome following de novo infection.

Published in PLoS Pathogens

The establishment of latency is an essential step for the life-long persistent infection and pathogenesis of Kaposi s sarcoma-associated herpesvirus (KSHV). While the KSHV genome is chromatin-free in the virions, the viral DNA in latently infected cells has a chromatin structure with activating and repressive histone modifications that promote late...

A viral kinase mimics S6 kinase to enhance cell proliferation.

Published in Proceedings of the National Academy of Sciences

Viruses depend upon the host cell for manufacturing components of progeny virions. To mitigate the inextricable dependence on host cell protein synthesis, viruses can modulate protein synthesis through a variety of mechanisms. We report that the viral protein kinase (vPK) encoded by open reading frame 36 (ORF36) of Kaposi s sarcoma-associated herpe...

KCNJ15/Kir4.2 couples with polyamines to sense weak extracellular electric fields in galvanotaxis.

Published in Nature Communications

Weak electric fields guide cell migration, known as galvanotaxis/electrotaxis. The sensor(s) cells use to detect the fields remain elusive. Here we perform a large-scale screen using an RNAi library targeting ion transporters in human cells. We identify 18 genes that show either defective or increased galvanotaxis after knockdown. Knockdown of the ...

Protein arginine methyltransferase 1-directed methylation of Kaposi sarcoma-associated herpesvirus latency-associated nu...

Published in Journal of Biological Chemistry

The Kaposi sarcoma-associated herpesvirus (KSHV) latency-associated nuclear antigen (LANA) is a multifunctional protein with roles in gene regulation and maintenance of viral latency. Post-translational modification of LANA is important for functional diversification. Here, we report that LANA is subject to arginine methylation by protein arginine ...

NF-kappaB serves as a cellular sensor of Kaposi s sarcoma-associated herpesvirus latency and negatively regulates K-Rta ...

Published in Journal of Virology

Successful viral replication is dependent on a conducive cellular environment; thus, viruses must be sensitive to the state of their host cells. We examined the idea that an interplay between viral and cellular regulatory factors determines the switch from Kaposi s sarcoma-associated herpesvirus (KSHV) latency to lytic replication. The immediate-ea...

ORF36 protein kinase of Kaposi s sarcoma herpesvirus activates the c-Jun N-terminal kinase signaling pathway.

Published in Journal of Biological Chemistry

alpha-, beta-, and gamma-Herpesviruses encode putative viral protein kinases. The herpes simplex virus UL13, varicella-zoster virus ORF47, and Epstein-Barr virus BGLF4 genes all show protein kinase domains in their protein sequences. Mutational analysis of these herpesviruses demonstrated that the viral kinase is important for optimal virus growth....

Kaposi s sarcoma-associated herpesvirus K-bZIP is a coregulator of K-Rta: physical association and promoter-dependent tr...

Published in Journal of Virology

Kaposi s sarcoma-associated herpesvirus (KSHV) is a human gammaherpesvirus that has been implicated in the pathogenesis of Kaposi s sarcoma and B-cell neoplasms. The genomic organization of KSHV is similar to that of Epstein-Barr virus (EBV). EBV encodes two transcriptional factors, Rta and Zta, which functionally interact to transactivate EBV gene...

Long non-coding RNA and epigenetic gene regulation of KSHV.

Published in Viruses

Kaposi s sarcoma-associated herpesvirus (KSHV/human herpesvirus 8) is a γ-herpesvirus linked to Kaposi s sarcoma (KS) and two lymphoproliferative disorders, primary effusion lymphoma (PEL or body-cavity B-lymphoma [BCBL]) and a subset of Multicentric Castleman s Disease. During lytic growth, pervasive viral transcription generating a variety of tra...

The binding of monomeric C-reactive protein (mCRP) to Integrins αvβ3 and α4β1 is related to its pro-inflammatory action....

The prototypic acute phase reactant C-reactive protein (CRP) is not only a marker but also a potential contributor to inflammatory diseases. CRP exists as the circulating native, pentameric CRP (pCRP) and the monomeric isoform (mCRP), formed as a result of a dissociation process of pCRP. mCRP is highly pro-inflammatory, but pCRP is not. The mechani...

PRMT4-mediated arginine methylation negatively regulates retinoblastoma tumor suppressor protein and promotes E2F-1 diss...

Published in Molecular and Cellular Biology

The retinoblastoma protein (pRb/p105) tumor suppressor plays a pivotal role in cell cycle regulation by blockage of the G1-to-S-phase transition. pRb tumor suppressor activity is governed by a variety of posttranslational modifications, most notably phosphorylation by cyclin-dependent kinase (Cdk) complexes. Here we report a novel regulation of pRb...

An siRNA Screen Identifies the U2 snRNP Spliceosome as a Host Restriction Factor for Recombinant Adeno-associated Viruse...

Published in PLoS Pathogens

Adeno-associated viruses (AAV) have evolved to exploit the dynamic reorganization of host cell machinery during co-infection by adenoviruses and other helper viruses. In the absence of helper viruses, host factors such as the proteasome and DNA damage response machinery have been shown to effectively inhibit AAV transduction by restricting processe...

KDM8, a H3K36me2 histone demethylase that acts in the cyclin A1 coding region to regulate cancer cell proliferation.

Published in Proceedings of the National Academy of Sciences

Localized chromatin modifications of histone tails play an important role in regulating gene transcription, and aberration of these processes leads to carcinogenesis. Methylated histone lysine residues, a key player in chromatin remodeling, are demethylated by the JmjC class of enzymes. Here we show that JMJD5 (now renamed KDM8), a JmjC family memb...