HALO384: a halo-based potency prediction algorithm for high-throughput detection of antimicrobial agents.
Published in Journal of Biomolecular Screening
A high-throughput (HT) agar-based halo assay is described, which allows for rapid screening of chemical libraries for bioactivity in microorganisms such as yeast and bacteria. A pattern recognition algorithm was developed to identify halo-like shapes in plate reader optical density (OD) measurements. The authors find that the total growth inhibitio...
