Ruiwu (Ray) Liu

The “One-Bead-One-Compound” Combinatorial Approach to Identifying Molecular Imaging Probes

High throughput screening of a small molecule one-bead-one-compound combinatorial library to identify attenuators of p21...

Published in Cancer Biology & Therapy

111In-LLP2A-DOTA Polyethylene Glycol–Targeting α4β1 Integrin: Comparative Pharmacokinetics for Imaging and Therapy of Ly...

Published in Journal of Nuclear Medicine

Peptide Combinatorial Libraries

Synthetic peptide and nonpeptide libraries. Encyclopedia of Molecular Cell Biology and Molecular Medicine

Novel peptide ligands for a4b1 integrin over-expressed in bronchiolalveolar cancer cells

Published in Molecular Cancer Therapeutics

Automatic Edman microsequencing of peptides containing multiple unnatural amino acids.

Published in Analytical Biochemistry

It is now routine using automatic Edman microsequencing to determine the primary structure of peptides or proteins containing natural amino acids; however, a deficiency in the ability to readily sequence peptides containing unnatural amino acids remains. With the advent of synthetic peptide chemistry, combinatorial chemistry, and the large number o...

Structure elucidation of peptides with unnatural amino acids using an automated protein sequencer In Peptides: The wave ...

Designed amino acids that induce b-sheet folding and b-sheet interactions in peptides

Development of peptidomimetic substrates and inhibitors that bind to the peptide binding pocket of the catalytic site of...

Increase diversity of sone-bead one-compound combinatorial peptide libraries with side-chain derivatization

Published in Genomics Proteomics & Therpaeutics

From One-Bead One-Compound Combinatorial Libraries to Chemical Microarrays

Congo red and thioflavin-T analogs detect Abeta oligomers.

Published in Journal of Neurochemistry

Several small molecule ligands for amyloid-beta (Abeta) fibrils deposited in brain have been developed to facilitate radiological diagnosis of Alzheimer s disease (AD). Recently, the build-up of Abeta oligomers (AbetaO) in brain has been recognized as an additional hallmark of AD and may play a more significant role in early stages. Evidence sugges...

Screening of a one bead-one compound combinatorial library for beta-actin identifies molecules active toward Ramos B-lym...

Published in Analytical Biochemistry

The search for small molecules that specifically recognize protein targets is a laborious process if conducted in a one protein-one compound manner. A high-throughput antibody-based screening of one bead-one compound (OBOC) combinatorial small molecule libraries is described here, whereby libraries containing thousands of different small molecule l...

In vivo optical imaging of human lymphoma xenograft using a library-derived peptidomimetic against alpha4beta1 integrin....

Published in Molecular Cancer Therapeutics

Increasing literature suggests that cell adhesion molecule alpha4beta1 integrin plays a pivotal role in autoimmune diseases and cancer development. Noninvasive visualization of alpha4beta1 integrin in vivo will facilitate the understanding of its involvement in disease progression and development of targeted therapies. Due to the lack of high-affin...

Alzheimer s disease drug candidates stabilize A-β protein native structure by interacting with the hydrophobic core.

Published in Biophysical Journal

Deposition of amyloid fibrils, consisting primarily of Aβ(40) and Aβ(42) peptides, in the extracellular space in the brain is a major characteristic of Alzheimer s disease (AD). We recently developed new (to our knowledge) drug candidates for AD that inhibit the fibril formation of Aβ peptides and eliminate their neurotoxicity. We performed all-ato...

Rapid discovery of death ligands with one-bead-two-compound combinatorial library methods.

Published in ACS Combinatorial Science

The one-bead-one-compound (OBOC) technology enables one to generate thousands to millions of chemical molecules on resin beads (90 μm diameter) such that each bead displays 10(13) copies of the same chemical entity. Whole-cell binding assays have been developed to screen OBOC combinatorial libraries for ligands that bind to specific cell surface re...

Directing mesenchymal stem cells to bone to augment bone formation and increase bone mass

Published in Nature Medicine

Molecular imaging of very late antigen-4 (α4β1 integrin) in the premetastatic niche.

Published in Journal of Nuclear Medicine

Covalent Chemical Ligation Strategy for Mono- and Polyclonal Immunoglobulins at Their Nucleotide Binding Sites.

Published in Bioconjugate Chemistry

Nonspecific ligation methods have been traditionally used to chemically modify immunoglobulins. Site-specific ligation of compounds (toxins or ligands) to antibodies has become increasingly important in the fields of therapeutic antibody-drug conjugates and bispecific antibodies. In this present study, we took advantage of the reported nucleotide-b...