Biased estimates of clonal evolution and subclonal heterogeneity can arise from PCR duplicates in deep sequencing experi...
Published in Genome Biology
Accurate allele frequencies are important for measuring subclonal heterogeneity and clonal evolution. Deep-targeted sequencing data can contain PCR duplicates, inflating perceived read depth. Here we adapted the Illumina TruSeq Custom Amplicon kit to include single molecule tagging (SMT) and show that SMT-identified duplicates arise from PCR. We de...
