Manuel Ares

A new alpha-helical extension promotes RNA binding by the dsRBD of Rnt1p RNAse III.

Published in The EMBO Journal

Rnt1 endoribonuclease, the yeast homolog of RNAse III, plays an important role in the maturation of a diverse set of RNAs. The enzymatic activity requires a conserved catalytic domain, while RNA binding requires the double-stranded RNA-binding domain (dsRBD) at the C-terminus of the protein. While bacterial RNAse III enzymes cleave double-stranded ...

Bacterial RNA isolation.

Published in Cold Spring Harbor Protocols

In this bacterial RNA isolation protocol, an "RNA-protective" treatment is followed by lysozyme digestion of the peptidoglycan component of the cell wall. EDTA promotes the loss of the outer membrane of Gram-negative bacteria and allows the lysozyme better access to the peptidoglycan. Cells begin to lyse during digestion in hypotonic lysozyme buffe...

Gene structure-based splice variant deconvolution using a microarray platform.

Published in Bioinformatics

Isolation of total RNA from yeast cell cultures.

Published in Cold Spring Harbor Protocols

This article describes two procedures for isolating total RNA from yeast cell cultures. The first allows the convenient isolation of total RNA from early log-phase cultures (vegetative cells). RNA isolated in this way is intact and sufficiently pure for use in microarray experiments, primer extension, and RNase protection mapping. With additional t...