Publications - Katsumi Morimatsu - Page 1

RecFOR proteins target RecA protein to a DNA gap with either DNA or RNA at the 5 terminus: implication for repair of st...

Katsumi Morimatsu Y, Wu Stephen Charles Kowalczykowski

Published in Journal of Biological Chemistry

The repair of single-stranded gaps in duplex DNA by homologous recombination requires the proteins of the RecF pathway. The assembly of RecA protein onto gapped DNA (gDNA) that is complexed with the single-stranded DNA-binding protein is accelerated by the RecF, RecO, and RecR (RecFOR) proteins. Here, we show the RecFOR proteins specifically target...

Escherichia coli RecX inhibits RecA recombinase and coprotease activities in vitro and in vivo.

Ea, Stohl Jp, Brockman Kl, Burkle Katsumi Morimatsu Stephen Charles Kowalczykowski Hs, Seifert

Published in Journal of Biological Chemistry

In Escherichia coli the RecA protein plays a pivotal role in homologous recombination, DNA repair, and SOS repair and mutagenesis. A gene designated recX (or oraA) is present directly downstream of recA in E. coli; however, the function of RecX is unknown. In this work we demonstrated interaction of RecX and RecA in a yeast two-hybrid assay. In vit...

RecQ helicase and RecJ nuclease provide complementary functions to resect DNA for homologous recombination.

Katsumi Morimatsu Stephen Charles Kowalczykowski

Published in Proceedings of the National Academy of Sciences

Recombinational DNA repair by the RecF pathway of Escherichia coli requires the coordinated activities of RecA, RecFOR, RecQ, RecJ, and single-strand DNA binding (SSB) proteins. These proteins facilitate formation of homologously paired joint molecules between linear double-stranded (dsDNA) and supercoiled DNA. Repair starts with resection of the b...

RecFOR proteins load RecA protein onto gapped DNA to accelerate DNA strand exchange: a universal step of recombinational...

Katsumi Morimatsu Stephen Charles Kowalczykowski

Published in Molecular Cell

Genetic evidence suggests that the RecF, RecO, and RecR (RecFOR) proteins participate in a common step of DNA recombination and repair, yet the biochemical event requiring collaboration of all three proteins is unknown. Here, we show that the concerted action of the RecFOR complex directs the loading of RecA protein specifically onto gapped DNA tha...

Reconstitution of initial steps of dsDNA break repair by the RecF pathway of E. coli.

Naofumi Handa Katsumi Morimatsu St, Lovett Stephen Charles Kowalczykowski

Published in Genes & Development

The RecF pathway of Escherichia coli is important for recombinational repair of DNA breaks and gaps. Here ;we reconstitute in vitro a seven-protein reaction that recapitulates early steps of dsDNA break repair using purified RecA, RecF, RecO, RecR, RecQ, RecJ, and SSB proteins, components of the RecF system. Their combined action results in process...