Pre-mRNA splicing: life at the centre of the central dogma.
Published in Journal of Cell Science
Multiple roles of arginine/serine-rich splicing factors in RNA processing.
Published in Biochemical Society Transactions
SR proteins (serine- and arginine-rich proteins) are an evolutionarily conserved family consisting of essential pre-mRNA splicing factors. Since their discovery and initial characterization, roles of SR proteins in pre-mRNA splicing and in subsequent steps of post-transcriptional gene expression have expanded significantly. The current hypotheses s...
Regulation of SR protein localization during development.
Published in Proceedings of the National Academy of Sciences
Ser-Arg-rich (SR) proteins play numerous roles in spliceosome assembly and the regulation of splice-site selection. Whereas considerable attention has focused on the mechanistic details of SR protein activities, little is known concerning how these splicing regulators are controlled by the cell. Here we examined the subcellular localization of prec...
Implementing telerehabilitation research for stroke rehabilitation with community dwelling veterans: lessons learned.
Published in International Journal of Telerehabilitation
Telerehabilitation (TR) is the use of telehealth technologies to provide distant support, rehabilitation services, and information exchange between people with disabilities and their clinical providers. This article discusses the barriers experienced when implementing a TR multi-site randomized controlled trial for stroke patients in their homes, a...
The splicing factor SF2/ASF regulates translation initiation by enhancing phosphorylation of 4E-BP1.
Published in Molecular Cell
The SR protein SF2/ASF has been initially characterized as a splicing factor but has also been shown to mediate postsplicing activities such as mRNA export and translation. Here we demonstrate that SF2/ASF promotes translation initiation of bound mRNAs and that this activity requires the presence of the cytoplasmic cap-binding protein eIF4E. SF2/AS...
Regulation of SR protein localization during development.
Published in Proceedings of the National Academy of Sciences
Ser-Arg-rich (SR) proteins play numerous roles in spliceosome assembly and the regulation of splice-site selection. Whereas considerable attention has focused on the mechanistic details of SR protein activities, little is known concerning how these splicing regulators are controlled by the cell. Here we examined the subcellular localization of prec...
Site identification in high-throughput RNA-protein interaction data.
Published in Bioinformatics
The Wilms tumour protein (WT1) shuttles between nucleus and cytoplasm and is present in functional polysomes.
Published in Human Molecular Genetics
Mutations of the Wilms tumour-1 (WT1) gene in humans can lead to childhood kidney cancer, life-threatening glomerular nephropathy and gonadal dysgenesis. The WT1 protein is normally expressed in the developing genitourinary tract, heart, spleen and adrenal glands and is crucial for their development, however it s function at the molecular level is...
Splicing factor SFRS1 recognizes a functionally diverse landscape of RNA transcripts.
Published in Genome Research
Metazoan genes are encrypted with at least two superimposed codes: the genetic code to specify the primary structure of proteins and the splicing code to expand their proteomic output via alternative splicing. Here, we define the specificity of a central regulator of pre-mRNA splicing, the conserved, essential splicing factor SFRS1. Cross-linking i...
Reversible phosphorylation differentially affects nuclear and cytoplasmic functions of splicing factor 2/alternative spl...
Published in Proceedings of the National Academy of Sciences
The Ser/Arg-rich (SR) proteins constitute a family of highly conserved nuclear phosphoproteins that are involved in many steps of mRNA metabolism. Previously, we demonstrated that shuttling SR proteins can associate with translating ribosomes and enhance translation of reporter mRNAs both in vivo and in vitro. Here, we show that endogenous, cytopla...
DNA Methyltransferase protein synthesis is reduced in CXXC finger protein 1-deficient embryonic stem cells.
Published in DNA and Cell Biology
CXXC finger protein 1 (CFP1) binds to unmethylated CpG dinucleotides and is required for embryogenesis. CFP1 is also a component of the Setd1A and Setd1B histone H3K4 methyltransferase complexes. Murine embryonic stem (ES) cells lacking CFP1 fail to differentiate, and exhibit a 70% reduction in global genomic cytosine methylation and a 50% reductio...
Reversible phosphorylation differentially affects nuclear and cytoplasmic functions of splicing factor 2/alternative spl...
Published in Proceedings of the National Academy of Sciences
The Ser/Arg-rich (SR) proteins constitute a family of highly conserved nuclear phosphoproteins that are involved in many steps of mRNA metabolism. Previously, we demonstrated that shuttling SR proteins can associate with translating ribosomes and enhance translation of reporter mRNAs both in vivo and in vitro. Here, we show that endogenous, cytopla...
Regulation of SR protein localization during development.
Published in Proceedings of the National Academy of Sciences
Ser-Arg-rich (SR) proteins play numerous roles in spliceosome assembly and the regulation of splice-site selection. Whereas considerable attention has focused on the mechanistic details of SR protein activities, little is known concerning how these splicing regulators are controlled by the cell. Here we examined the subcellular localization of prec...
A novel role for shuttling SR proteins in mRNA translation.
Published in Genes & Development
The Ser-Arg-rich (SR) proteins comprise a large family of nuclear phosphoproteins that are required for constitutive and alternative splicing. A subset of SR proteins shuttles continuously between the nucleus and the cytoplasm, suggesting that the role of shuttling SR proteins in gene expression may not be limited to nuclear pre-mRNA splicing, but ...
Effect of the E200K mutation on prion protein metabolism. Comparative study of a cell model and human brain.
Published in American Journal Of Pathology
The hallmark of prion diseases is the cerebral accumulation of a conformationally altered isoform (PrP(Sc)) of a normal cellular protein, the prion protein (PrP(C)). In the inherited form, mutations in the prion protein gene are thought to cause the disease by altering the metabolism of the mutant PrP (PrP(M)) engendering its conversion into PrP(Sc...
Site identification in high-throughput RNA-protein interaction data.
Published in Bioinformatics
Antimicrobial Peptide LL37 and MAVS Signaling Drive Interferon-β Production by Epidermal Keratinocytes during Skin Injur...
Published in Immunity
Type 1 interferons (IFNs) promote inflammation in the skin but the mechanisms responsible for inducing these cytokines are not well understood. We found that IFN-β was abundantly produced by epidermal keratinocytes (KCs) in psoriasis and during wound repair. KC IFN-β production depended on stimulation of mitochondrial antiviral-signaling protein (M...
Frac-seq reveals isoform-specific recruitment to polyribosomes.
Published in Genome Research
Pre-mRNA splicing is required for the accurate expression of virtually all human protein coding genes. However, splicing also plays important roles in coordinating subsequent steps of pre-mRNA processing such as polyadenylation and mRNA export. Here, we test the hypothesis that nuclear pre-mRNA processing influences the polyribosome association of ...
Pre-mRNA splicing: life at the centre of the central dogma.
Published in Journal of Cell Science
Multiple roles of arginine/serine-rich splicing factors in RNA processing.
Published in Biochemical Society Transactions
SR proteins (serine- and arginine-rich proteins) are an evolutionarily conserved family consisting of essential pre-mRNA splicing factors. Since their discovery and initial characterization, roles of SR proteins in pre-mRNA splicing and in subsequent steps of post-transcriptional gene expression have expanded significantly. The current hypotheses s...
