Huan-Yuan Chen

Endogenous galectin-3 is localized in membrane lipid rafts and regulates migration of dendritic cells

Published in Journal of Investigative Dermatology Symposium Proceedings

Galectin-3 negatively regulates TCR-mediated CD4+ T cell activation at the immunological synapse

Published in Proc Natl Acad Sci

Galectin-3 regulates T cells

Published in Immunological Reviews

Galectin-3 is critical for the development of the allergic inflammatory response in a mouse model of atopic dermatitis

Published in American Journal Of Pathology

Role of galectin-3 in mast cell functions. Galectin-3-deficient mast cells exhibit impaired mediator release and defecti...

Published in Journal of Japan Society of Immunology & Allergology in Otolaryngology

Galectin-12 is Required for Adipogenic Signaling and Adipocyte Differentiation

Published in JBIC Journal of Biological Inorganic Chemistry

Critical role for galectin-3 in airway inflammation and bronchial hyperresponsiveness in a murine model of asthma

Published in American Journal Of Pathology

Engineered Nanostructures of Haptens Lead to Unexpected Formation of Membrane Nanotubes Connecting Rat Basophilic Leukem...

Published in ACS Nano

A recent finding reports that co-stimulation of the high-affinity immunoglobulin E (IgE) receptor (FcεRI) and the chemokine receptor 1 (CCR1) triggered formation of membrane nanotubes among bone-marrow-derived mast cells. The co-stimulation was attained using corresponding ligands: IgE binding antigen and macrophage inflammatory protein 1α (MIP1 α)...

Roles of galectin-3 in immune responses.

Published in Archivum Immunologiae et Therapiae Experimentalis

Galectins are a family of animal lectins with conserved carbohydrate-recognition domains for beta-galactoside. Galectin-3 is the only family member that is composed of a glycine/prolinerich N-terminal repeated sequence and a C-terminal carbohydrate-binding domain.Multiple functions of galectin-3 have been reported, depending on its location. Extrac...

Galectin-3 regulates T-cell functions.

Published in Immunological Reviews

Galectin-3 is absent in resting CD4+ and CD8+ T cells but is inducible by various stimuli. These include viral transactivating factors, T-cell receptor (TCR) ligation, and calcium ionophores. In addition, galectin-3 is constitutively expressed in human regulatory T cells and CD4+ memory T cells. Galectin-3 exerts extracellular functions because of ...

Cell mechanics using atomic force microscopy-based single-cell compression.

Published in Langmuir

We report herein the establishment of a single-cell compression method based on force measurements in atomic force microscopy (AFM). The high-resolution bright-field or confocal laser scanning microscopy guides the location of the AFM probe and then monitors the deformation of cell shape, while microsphere-modified AFM probes compress the cell and ...

Galectin-7 Regulates Keratinocyte Proliferation and Differentiation through JNK-miR-203-p63 Signaling.

Published in Journal of Investigative Dermatology

Galectin-7, a member of the β-galactoside-binding protein family, is primarily expressed in stratified epithelial cells, including keratinocytes. There is information in the literature suggesting a role for this protein in regulation of keratinocyte survival and growth, but the underlying mechanism remains relatively unknown. Moreover, its expressi...

Impact of actin rearrangement and degranulation on the membrane structure of primary mast cells: a combined atomic force...

Published in Biophysical Journal

Degranulation of bone marrow-derived mast cells (BMMCs) triggered by antigens (e.g., 2,4-dinitrophenylated bovine serum albumin (DNP-BSA) and secretagogues (e.g., poly-L-lysine) was investigated by combined atomic force microscopy (AFM) and laser scanning confocal microscopy (LSCM). This combination enables the simultaneous visualization and correl...

Galectin-3 and the skin.

Published in Journal of Dermatological Science

Galectin-3 is highly expressed in epithelial cells including keratinocytes and is involved in the pathogenesis of inflammatory skin diseases by affecting the functions of immune cells. For example, galectin-3 can contribute to atopic dermatitis (AD) by promoting polarization toward a Th2 immune response by regulating dendritic cell (DC) and T cell ...

IgE, mast cells, and eosinophils in atopic dermatitis.

Published in Clinical Reviews in Allergy & Immunology

Atopic dermatitis (AD) is a chronic inflammatory skin disease with specific immune and inflammatory mechanisms. Atopy is among the major features of the diagnosis criteria for AD but is not an essential feature. Thus, patients diagnosed with AD can be atopic or non-atopic. This review focuses on the role of IgE, mast cells, and eosinophils in the p...

Galectin-3 modulates Th17 responses by regulating dendritic cell cytokines.

Published in American Journal Of Pathology

Galectin-3 is a β-galactoside-binding animal lectin with diverse functions, including regulation of T helper (Th) 1 and Th2 responses. Current data indicate that galectin-3 expressed in dendritic cells (DCs) may be contributory. Th17 cells have emerged as critical inducers of tissue inflammation in autoimmune disease and important mediators of host...

Galectin-3 regulates intracellular trafficking of EGFR through Alix and promotes keratinocyte migration.

Published in Journal of Investigative Dermatology

The EGFR-mediated signaling pathways are important in a variety of cellular processes, including cell migration and wound re-epithelialization. Intracellular trafficking of EGFR is critical for maintaining EGFR surface expression. Galectin-3, a member of an animal lectin family, has been implicated in a number of physiological and pathological proc...

Examination of galectins in phagocytosis.

Published in Methods in Molecular Biology

Galectins, a family of β-galactoside-binding proteins, are expressed in many different phagocytic leukocytes (granulocytes, monocytes, and macrophages). A number of family members have been shown to play an important role in ingestion of particles (phagocytosis), thus contributing to clearance of damaged cells and host defense against pathogens. He...

Galectin-3 promotes HIV-1 budding via association with Alix and Gag p6.

Published in Glycobiology

Galectin-3 has been reported to regulate the functions of a number of immune cell types. We previously reported that galectin-3 is translocated to immunological synapses in T cells upon T-cell receptor engagement, where it associates with ALG-2-interacting protein X (Alix). Alix is known to coordinate with the endosomal sorting complex required for...

Engineered nanostructures of antigen provide an effective means for regulating mast cell activation.

Published in ACS Nano

Nanostructures containing 2,4-dinitrophenyl (DNP) as antigen were designed and produced to investigate antibody-mediated activation of mast cells. The design consists of nanogrids of DNP termini inlaid in alkanethiol self-assembled monolayers (SAMs). Using scanning probe-based nanografting, nanometer precision was attained for designed geometry, si...