Concurrent dermatomyositis and metastatic breast carcinoma: case report.
Published in Henry Ford Hospital medical journal
Doug Kellogg
Institution:
UCSC Cancer, biomedical-ucsc
Cdk1 coordinates cell-surface growth with the cell cycle.
Published in Nature Cell Biology
The mechanisms that control cell growth during the cell cycle are poorly understood. In budding yeast, cyclin dependent kinase 1 (Cdk1) triggers polarization of the actin cytoskeleton and bud emergence in late G1 through activation of the Cdc42 GTPase. However, Cdk1 is not thought to be required for subsequent growth of the bud. Here, we show that ...
Compact modeling of allosteric multisite proteins: application to a cell size checkpoint.
Published in PLoS Computational Biology
We explore a framework to model the dose response of allosteric multisite phosphorylation proteins using a single auxiliary variable. This reduction can closely replicate the steady state behavior of detailed multisite systems such as the Monod-Wyman-Changeux allosteric model or rule-based models. Optimal ultrasensitivity is obtained when the activ...
MBoC 2011: same values, improved feng shui.
Published in Molecular Biology of the Cell
Protein- and immunoaffinity purification of multiprotein complexes.
Published in G Protein Coupled Receptors - Structure
The Sda1 protein is required for passage through start.
Published in Molecular Biology of the Cell
We have used affinity chromatography to identify proteins that interact with Nap1, a protein previously shown to play a role in mitosis. Our studies demonstrate that a highly conserved protein called Sda1 binds to Nap1 both in vitro and in vivo. Loss of Sda1 function causes cells to arrest uniformly as unbudded cells that do not increase significan...
Centrosomes. Organizing cytoplasmic events.
Published in Nature
Studies on the cytoplasmic organization of early Drosophila embryos.
Published in Cold Spring Harbor Symposia on Quantitative Biology
English as the universal language of science: opportunities and challenges.
Published in Molecular Biology of the Cell
Drosophila Wee1 interacts with members of the gammaTURC and is required for proper mitotic-spindle morphogenesis and pos...
Published in Current Biology
Identification of microtubule-associated proteins in the centrosome, spindle, and kinetochore of the early Drosophila em...
Published in The Journal of Cell Biology
We have developed affinity chromatography methods for the isolation of microtubule-associated proteins (MAPs) from soluble cytoplasmic extracts and have used them to analyze the cytoskeleton of the early Drosophila embryo. More than 50 Drosophila embryo proteins bind to microtubule affinity columns. To begin to characterize these proteins, we have ...
Biochemical and genetic characterization of Yra1p in budding yeast.
Published in Yeast
Yra1p and its vertebrate homologues bind to the mRNA export factor Mex67p/TAP and are thought to play a role in mRNA export in vivo. To further characterize Yra1p, we used immunoaffinity chromatography to purify endogenous Yra1p complexes. These experiments demonstrated that two importin beta homologues (Kap123p and Pse1p) and the poly A tail-bindi...
Plasma membrane growth during the cell cycle: unsolved mysteries and recent progress.
Published in Current Opinion in Cell Biology
Growth of the plasma membrane is as fundamental to cell reproduction as DNA replication, chromosome segregation and ribosome biogenesis, yet little is known about the underlying mechanisms. Membrane growth during the cell cycle requires mechanisms that control the initiation, location, and extent of membrane growth, as well as mechanisms that coord...
Nucleocytoplasmic transport in the midzone membrane domain controls yeast mitotic spindle disassembly.
Published in The Journal of Cell Biology
During each cell cycle, the mitotic spindle is efficiently assembled to achieve chromosome segregation and then rapidly disassembled as cells enter cytokinesis. Although much has been learned about assembly, how spindles disassemble at the end of mitosis remains unclear. Here we demonstrate that nucleocytoplasmic transport at the membrane domain su...
Regulation of Mih1/Cdc25 by protein phosphatase 2A and casein kinase 1.
Published in The Journal of Cell Biology
The Cdc25 phosphatase promotes entry into mitosis by removing cyclin-dependent kinase 1 (Cdk1) inhibitory phosphorylation. Previous work suggested that Cdc25 is activated by Cdk1 in a positive feedback loop promoting entry into mitosis; however, it has remained unclear how the feedback loop is initiated. To learn more about the mechanisms that regu...
Cdc28-dependent regulation of the Cdc5/Polo kinase.
Published in Current Biology
Polo kinase is activated as cells enter mitosis and plays a central role in coordinating diverse mitotic events, yet the mechanisms leading to activation of Polo kinase are poorly understood . Work in Xenopus meiotic cell cycles has suggested that Polo kinase functions in a pathway that helps trigger activation of Cdk1 . However, studies in other o...
PP2ARts1 is a master regulator of pathways that control cell size.
Published in The Journal of Cell Biology
Cell size checkpoints ensure that passage through G1 and mitosis occurs only when sufficient growth has occurred. The mechanisms by which these checkpoints work are largely unknown. PP2A associated with the Rts1 regulatory subunit (PP2A(Rts1)) is required for cell size control in budding yeast, but the relevant targets are unknown. In this paper, w...
Wee1-dependent mechanisms required for coordination of cell growth and cell division.
Published in Journal of Cell Science
Wee1-related kinases function in a highly conserved mechanism that controls the timing of entry into mitosis. Loss of Wee1 function causes fission yeast and budding yeast cells to enter mitosis before sufficient growth has occurred, leading to formation of daughter cells that are smaller than normal. Early work in fission yeast suggested that Wee1 ...
Electrical characterization of gel collected from shark electrosensors.
Published in Physical Review E
To investigate the physical mechanism of the electric sense, we present an initial electrical characterization of the glycoprotein gel that fills the electrosensitive organs of marine elasmobranchs (sharks, skates, and rays). We have collected samples of this gel, postmortem, from three shark species, and removed the majority of dissolved salts in ...
Cks confers specificity to phosphorylation-dependent CDK signaling pathways.
Published in Nature Structural & Molecular Biology
Cks is an evolutionarily conserved protein that regulates cyclin-dependent kinase (CDK) activity. Clarifying the underlying mechanisms and cellular contexts of Cks function is critical because Cks is essential for proper cell growth, and its overexpression has been linked to cancer. We observe that budding-yeast Cks associates with select phosphory...
